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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus]
    #27523713 - 10/30/21 06:27 AM (2 years, 2 months ago)

RE fast moving cultures:

This question is for everyone in order to understand how fast a “good” culture is vs whatever we are working with. So the question is: How long does it take for your “good” plates to grow all the way out to the edge (most don’t let them get to the edge so add on an extra day or two for that), what is your plate diameter and how big was your transfer plug? Second question for everyone is how long does it take your grain jars to fully consolidate (from agar wedge inoculation)?

I’m not home now and need my notes to be certain but from memory I think my “best” pan cultures are:
Agar: 66mm plate, 1/4” plug transfer, 7-9 days to edge
Grain Jar: 12-14 days (maybe I had one finish in 10 days - need to double check).

My fastest grain jar was following JOCs grain Tek to the letter. IMO Pans are 10x more finicky about grain hydration than cubes. Before using JOCs grain prep method, every stalled grain jar I had was fixed by injecting 10-20cc of sterile water and shaking so my grains were too dry or losing moisture too quickly through the SFD.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #27523738 - 10/30/21 06:58 AM (2 years, 2 months ago)

Quote:

ruawakeyet said:
Oops, I didn't mean to imply JOC suggested to colonize the bulk in the bag. I thought up that mistake all by myself. I was originally planning on only using the bags to mix in, but for some reason that escapes me now, I didn't have trays sanitized and ready to lay to.

I figured the bags would be fine as the zipper had too many little pieces of manure for the zipper to seal. I just loosely rolled the tops, but apparently that wasn't enough air exchange.

My first attempt and only success came from colonizing in the trays, covered with foil, with 6 holes poked in the foil using a needle. I assumed they didn't get much air exchange under the foil.

Jake, IIRC, you colonize your trays with lids on, not latched, right? Do you have holes in the lids? Didn't you say you will lift the lid in front of your flowhood sometimes halfway through colonization?

I should have taken notes while I was reading. This entire thread + the official pan thread on that other site + JOC TEK + everything Asura wrote + some archived stuff = 1 scrambled memory

What are your latest thoughts on layering spawn / bulk vs. mixing?




Yes the trees have lids on them but they don’t have gaskets or anything I’m sure a small amount of air is allowed through I will also take the trays and open them up in front of the flow hood a few times during the colonization process shut up as for mixing it together I just mix the spawn and the bulk really well I haven’t done Layering with pans


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27523919 - 10/30/21 10:32 AM (2 years, 2 months ago)

Guys, I need some advice again (THOSE OF YOU WHO HAVE HAD SUCCESS WITH BISPORUS).

So, my new culture from CM also failed, despite his beautiful grows. I have no idea what I am doing wrong, but it seems to me that:

1. Bisporus may actually need to overlay the casing in order to start pinning

2. You must wet the casing but then leave it without misting because misting damages the surface myc

I have waited a couple days after full colonization before I cased, and then I misted it several times. But it's been 12 days since casing and no signs of life (other than green molds).

Help pls!


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27523965 - 10/30/21 11:14 AM (2 years, 2 months ago)

Quote:

Hindsight said:
RE fast moving cultures:

IMO Pans are 10x more finicky about grain hydration than cubes. Before using JOCs grain prep method, every stalled grain jar I had was fixed by injecting 10-20cc of sterile water and shaking so my grains were too dry or losing moisture too quickly through the SFD.




You can tell if your grain is too wet because the myc will basically grow into a dense ball around the wedge
and not really spread throughout the jar.

I've been running on millet lately. After prep, I dump it all into a giant dough proofing tray and let it sit
for 12 hours or so. Stir it around here and there. All my latest jars are colonizing in 12-14 days.
I had to force myself to let the grain dry this long because it doesn't seem right. But it is.


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus]
    #27523973 - 10/30/21 11:21 AM (2 years, 2 months ago)

Quote:

rockyfungus said:
@ru Where ya getting your spores. I may be totally wrong. I got absolutely fucking nowhere till I tracked down a reliable print.





I completely agree with you there, I credit my only success to the quality of the spore print. I'm running Jake's PHV that I bought from a trusted source. (My all time favorite, which I can't name...)

In fact, they are so good, I had some small scale success making my own syringe and inoculating directly with spores into some BJ style manure cakes, and even directly to grain. I only had room to fruit 4 manure pf cakes, and one meal prep tray using manure and verm for bulk. Succulent soil was used for casing, and everything fruited without contamination in a make shift hybrid FC. Only yielded around 2.5 grams dry, but that small success was what I needed to see before investing money to build an automated fogger / evap system.

I only tried that route because I was waiting on ingredients for agar and still reading and learning and deciding what route I was going to take. I couldn't stand not doing something with the spores after they arrived, and I didn't have any expectations other than learning some new stuff. That is how I ended up having a clone to work with alongside some T1 MS for my first real grow.

Unfortunately, after some fantastic beginner's luck, I'm now struggling to the point of throwing in the towel, at least until warmer temperatures return. I can't really justify maintaining mid to upper 70s when it's cold outside and not seeing enough productivity.

The only thing keeping me going for now is the desire to not run out of my microdose supply, and the fact that I finally have some fatties coming up. It's weird, my clone specimen and the first tray of multiple flushes were all skinny fruits. Now I have some fat stemmed fruits that look promising, but the pinset is less than desirable. Seems that is a common trade off, but I would love to get a canopy of fat fruits.

I'll post a pic later today or tomorrow, once they reach their fullest glory.

Hindsight, re speed of cultures: I use 1/2 pint WM jars for agar plates, which is less than ideal but the ability to reuse them has motivated me to learn to deal with them. Anyway, I don't even bother writing down dates any more. Not for agar; grain; nor bulk. Everything is so slow that it's depressing when I read about cultures eating a plate in less than a week. My cultures don't eat a plate in less than 2 weeks, more like 2 1/2. Myco pints of grain have been averaging about the same, and I've just switched to using quarts so I'll see if I like that or not.

Despite not having the speed that everyone suggests looking for, I had to attempt to fruit anyway because if I hadn't, I would still be doing nothing other than playing with agar after months of work.


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Offlinerockyfungus
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #27524003 - 10/30/21 11:51 AM (2 years, 2 months ago)

Go back to spores if you got them. Your wasting time and materials.

Gotta run a bunch of plates. I'll germ 2 plates. Streak 3 bigger patches from each. Only use 3-4 that outgrow the rest.

Here. I put agar to grain 8 days ago.

this is day 3 or 4. Last night they were colonized the whole side and bottom so time for a shake.

6ish hours ago I shook the fuck out of these and dropped new agar to grain maybe 12ish hours? Bit nervous was pissed and shook them hard seem some bruising.
New agar is not even 24 hours out and already moving!


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus] * 1
    #27524379 - 10/30/21 04:39 PM (2 years, 2 months ago)

First pins popped up over night in an 8 quart mono. Seeing lots of knots on 50% of the surface.



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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27525245 - 10/31/21 11:18 AM (2 years, 2 months ago)

Baba, I'm still impressed by your ability to grow pans in monos. Looking forward to seeing a photo before you harvest!

Here is my latest: I tried to have 9 trays for this round, but lost 3 to contaminates, and lost 1 to a poor culture that didn't colonize the bulk. (I've been trying to get a Peace River cleaned up on agar, but it's been giving me issues. Everything else has been PHV clone culture I'm playing with.) Those 4 trays never even got cased.

I just harvested the first flush from 2 trays. (These are my 'B' culture from clone) They didn't have a good pinset, but I did harvest the largest pans I've had yet, and even had a decent cluster. I am printing and cloning the 2 largest fruits. (Yes, I'm taking a clone from a clone, which I wouldn't normally do until I read what Asura said recently.) Unfortunately I'm seeing what I think is a contaminate on each tray, but I'm hoping it's just some light overlay...

One of the trays is a dud, I guess that subculture just sucks. (This was my 'D' culture from the same clone) It colonized the fastest out of 4 subcultures, but only has one pin and was cased at the same time as the 2 good trays. I also see a contaminate or possibly some overlay. I'm close to tossing that one, but I'm letting it go for now... (My dumbass didn't want to wait to see which culture performed the best before inoculating my next round of grain jars. I chose the 'D' culture, which means I will likely be tossing ALL of my current grain jars and starting over with 'B', or one of my newest clones depending on how they take... IDK yet.)

I still have 2 more 'B' trays that should be pinning soon. They were a little slower than the first two, so they got cased and put into FC several days later. These are looking good, I don't see anything bad in the casing layer, (yet). If I'm lucky, what I suspect are contaminates in the first 2 trays are only overlay, and all 4 of these 'B' trays will give me multiple flushes. However, 4 out of 9 is very frustrating to me, and I'm not that hopeful I only have overlay. I may end up with only one flush from 2 trays, leaving only 2 trays left...





Here's that fat single on the scale: (My previous heaviest was 3.0 wet.)


Looking at this photo, I'm pretty sure that's trich coming up. I will be watching this closely...




Here's the heaviest fruit from the cluster on the scale:


I don't know if it's because these stems are so much thicker than anything I've harvested so far, but the bruising is MUCH more pronounced and immediate when I cut them up. Based on the little piece I ate for today's microdose, these might be even more potent than the skinny fruit I've grown. I suspect just one of these fatties is going to be WAY too much for a micro, where I've been eating 2 to 4 skinny ones previously.

I am REALLY looking forward to my next macro with some of these fatties!


Edited by ruawakeyet (10/31/21 11:43 AM)


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OfflineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #27525344 - 10/31/21 01:04 PM (2 years, 2 months ago)

Have you tried adding more holes to the mono??


They obviously really like the airflow coming from them based on those clusters. I still gotta try running a mono soon.



Those trays are beautiful too ruawake. Some giants.


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #27525362 - 10/31/21 01:18 PM (2 years, 2 months ago)

Nice rua, you got an entry for the Official 5+g thread there :lol:


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a] * 1
    #27525494 - 10/31/21 04:03 PM (2 years, 2 months ago)

Quote:

A.k.a said:
Have you tried adding more holes to the mono??


They obviously really like the airflow coming from them based on those clusters. I still gotta try running a mono soon.



Those trays are beautiful too ruawake. Some giants.





No not yet, I will run my old monos till they break and then I'll try a few with different hole setup. I probably buy a few more of those 8
quart tiny tubs though, they are nice for trying different things as you can run them with 1/2 to 1/4 quart of spawn.

I ran probably a bit over 30 monos with old school hole setup so far and the first or biggest clusters are not always directly where the
holes are. It probably would be of advantage to have more smaller holes above sub level to get a more even distribution of fresh air. I really
need to get those clones going before making any assumptions, poured 2 different clone LCs yesterday and dropped 3 more clones to LC broth. For some
reason my cultures are a bit sluggish on grain before the first shake, takes ages to get them colonized but they do great once they hit the bulk.

The tiny tub from above 24hrs later.




This is one of the bigger ones I'm running at the moment and I had nothing over the holes for almost 48 hrs. Lots of side pins so far but
nothing on top apart from a few visible knots, this has never happened to this extend before. Who knows why, it's MS after all.



Edited by Baba Yaga (10/31/21 04:23 PM)


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27525503 - 10/31/21 04:14 PM (2 years, 2 months ago)

That's funny, never seen Pans with so many side pins! That's some Cube-like genetics. Definitely keep them.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27525506 - 10/31/21 04:17 PM (2 years, 2 months ago)

Hey ruawake, those fruits are beautiful, just keep at it, it will be getting better. Just don't get too hung up about what kind of manure you're using or what brand of straw, it's not an exact science in this respect. I am using non leached manure at the moment, just picked it up and threw it in a bucket which is under my porch with lots of critters and larvae in it and I use coir instead of straw so go figure.

Focus on culture work and keep the rest the same for a while. I usually fail a couple of times after my breaks from cult but that's part of the
hobby and I run a few cube bottles just to have something to look at around.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #27525508 - 10/31/21 04:19 PM (2 years, 2 months ago)

Quote:

Adas said:
That's funny, never seen Pans with so many side pins! That's some Cube-like genetics. Definitely keep them.





I'll usually get side pins later on but not that early, this tub is even pinning in areas that are submerged in condensation water
accumulating at the bottom.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #27526409 - 11/01/21 01:51 PM (2 years, 2 months ago)

Pan cam Jamaica


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27526412 - 11/01/21 01:55 PM (2 years, 2 months ago)

Jake, those are beautiful as usual! I see some super fat stems on some of those.

Are those are MS or clone?


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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #27526444 - 11/01/21 02:31 PM (2 years, 2 months ago)

Quote:

Adas said:
Guys, I need some advice again (THOSE OF YOU WHO HAVE HAD SUCCESS WITH BISPORUS).

So, my new culture from CM also failed, despite his beautiful grows. I have no idea what I am doing wrong, but it seems to me that:

1. Bisporus may actually need to overlay the casing in order to start pinning

2. You must wet the casing but then leave it without misting because misting damages the surface myc

I have waited a couple days after full colonization before I cased, and then I misted it several times. But it's been 12 days since casing and no signs of life (other than green molds).

Help pls!



12 days isn't too long. Bisporus moves slower than cyans. Sometimes a lot slower. But green is not a good sign 😕


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: nosf3r4tu]
    #27526459 - 11/01/21 02:39 PM (2 years, 2 months ago)

Thanks sir, I will keep trying!


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27526497 - 11/01/21 03:12 PM (2 years, 2 months ago)

Thanks this is a clone tray


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419] * 3
    #27527123 - 11/02/21 05:44 AM (2 years, 2 months ago)

sup yall just poppin in to say hello😃 lots of nice look pics lately im glad to c ppl are doin well. im still workin at all kinds of shit but i hope to b wrapped up and back at it in another month or so🤞


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