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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: myco.bay] * 1
    #27521000 - 10/28/21 02:52 AM (2 years, 3 months ago)

Quote:

myco.bay said:
Hi, it's my first attempt at pans and I'm worried about one of my tubs. The right side of the image looks like healthy mycelium to me but I'm not too sure about the left, it looks a bit different.






On the left that's mold. You still might get fruits tho, doesn't look like Trich so it shouldn't kill your grow.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27521228 - 10/28/21 09:19 AM (2 years, 3 months ago)

Looking for any input on my trays as well.

Each tray: 4 cups of extremely dry horse manure (so dry it put dust into the air when worked), 100g of shredded straw (same brand JOC uses), 2/3 cup verm. Brought to field capacity (squeeze and 5-10 drops come out), put in a ziploc bag and instapot pasteurized for 12 hours, then left on the counter to age 7 days.

Opened bag, added water to bring substrate back to field capacity (~3-5 drops when squeezed). Mixed one full grain jar for each tray in clean plastic tote. My grain jars have just over 1 pint in them - about 1.3 pints which I estimate to be about a 1:4 grain to sub ratio by eyeballing.

I didn't read about not having exposed grains in the trays until yesterday, so in the future I'll ensure I cover the grains with substrate.

Trays were covered with the plastic snap-on covers and left in the grow closet at around 78-80F.

This is about the best looking tray I have but still don't like it. 8 days old:



4 day old tray here. You can see 100% consolidation but note how thin the mycelium is - not dense fluffy bright white all over:


The worst tray - 8 days old:



My spawn is definitely clean. I've determined my previous spawn issues were due to them being too dry. I checked for bacteria by injecting water into the grain jar, shaking, pulling it back out, then putting to agar. No bacteria, only mycelium growth.

The only thing I can think of doing differently at this point is allowing more airflow during consolidation, and trying a different manure source, though I've read a lot from RR about manure and that about the only issue you would have with it is ammonia, but he says ammonia will be gone once the manure is fully dried. He stated leeching doesn't remove the ammonia. I'm buying my manure dried and aged so have no idea what process was followed. From here, all I can think to do is go up to the horse farms and get permission from one to gather a tub full of field aged manure pellets, spread them out to dry a bit at home, and then start using that as my manure source.

Open and appreciative to any direction here. Aside from pissing me off, all these failures do is further strengthen my resolve to figure this out :smile:


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27521308 - 10/28/21 10:25 AM (2 years, 3 months ago)

what is it you don't like about this? have you had them colonize differently before?

IME the airier you make the substrate the more wispier it will colonize.

how do they perform in the FC?


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27521352 - 10/28/21 11:06 AM (2 years, 3 months ago)

Thanks Baba. I have only successfully fruited one pan cyan tray so far in the fruiting tent so I am a pan newbie. When I say it doesn’t look good, I’m comparing to photos I see in this thread. Most people’s trays seem to consolidate nearly completely in 2-4 days and they look like someone layered pure white cotton over the top. Vs mine which still don’t have full coverage after 8 days (in some cases) and have a much less fluffy and less bright white mycelium.

I did tamp the substrate in these trays down a bit more than I normally would. I’ll do that less next time.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27521390 - 10/28/21 11:41 AM (2 years, 3 months ago)

Tamping down is good, I meant it like the airier the sub the thinner the colonization, at least that is what I've experienced.
My tubs take about 10 days incl. a couple of days of consolidation and it's like thick white cotton but I only ever grew that
one cambo so far so this could be variety specific and I am using H-manure and coir.

Does this happen with all your different pans?

Your trays look more like Asuras sub after it recovers from being transferred from bags to trays.

TBH I don't think that there is anything wrong with it yet, just see how it performs.


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27521444 - 10/28/21 12:11 PM (2 years, 3 months ago)

I do remember Jake talking about the quality of manure matters. Grass only or hay only fed horses makes for the best poo, if you see a lot of grain in it, it can negatively affect the outcome. A lot of the manure I picked up last time had quite a few oats in it, so that might be the source of some problems. I also think age has a lot to do with it. Seems like the turds should be starting to break down and lose the turd shape before it's prime. Jake's manure pile looks more like top soil than it does manure. I need to make a pile outside with what I have and let it age some more.

I agree with Baba, as long as it colonizes, case it and throw it into fruiting.

Then again, I'm reluctant to even reply due to my recent high failure rate.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #27521504 - 10/28/21 12:55 PM (2 years, 3 months ago)

Thanks Baba and Ruawakeyet - Maybe I'm concerned over nothing then. I will just let them ride and see what happens (except for the one really bad tray which just looks completely dead).

Baba, yes this happens with all my different pans. I'm trying to grow around 7 varieties now, but have only gotten nice strong cultures and good clean grain jars for three or four of them so far, and the trays do vary a little bit by variety in terms of look, for example, the Alabama has the most dense cottony white mycelium (even though it's patchy and doesn't fully cover the substrate - yet).

I am also using horse manure (all bought online for now). Here is a pic of the stuff I've been using (on the left) vs the new stuff I just got in (on the right). I've yet to spawn a tray of the manure on the right but I should be doing that this coming Monday. Ruawakeyet, it's interesting you mention Jake's looking like top soil because that's exactly what the new stuff I bought looks like as you can see from the photo, vs the previous stuff I was using on the left looking more "grassy". Do note that the new manure on the right is a bit damp which is part of why it appears lighter, but I bet it's darker and more top-soil looking then the older stuff on the left even if dried out completely.





I have already pasteurized one tray's worth of the new manure+straw and am aging it for spawning on Monday. I'll post whether or not it works any differently than the previous manure.


Edited by Hindsight (10/28/21 01:00 PM)


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InvisibleGlitchInSpace
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27521810 - 10/28/21 05:11 PM (2 years, 3 months ago)

Quote:

Hindsight said:
Looking for any input on my trays as well.

Each tray: 4 cups of extremely dry horse manure (so dry it put dust into the air when worked), 100g of shredded straw (same brand JOC uses), 2/3 cup verm. Brought to field capacity (squeeze and 5-10 drops come out), put in a ziploc bag and instapot pasteurized for 12 hours, then left on the counter to age 7 days.




what name brand does joc use for his shredded straw?


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: GlitchInSpace] * 3
    #27521828 - 10/28/21 05:27 PM (2 years, 3 months ago)



--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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OfflineNichrome
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27521843 - 10/28/21 05:45 PM (2 years, 3 months ago)

Ohh products.... What's in it?


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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Offlinemyco.bay
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27521852 - 10/28/21 05:50 PM (2 years, 3 months ago)

Thank you Adas for the reply, I'll get rid of it just to be safe an not risk having it move onto my other grows too.

I totally forgot that today is the day Jake is making his sub available. I'm excited to try it out in the future.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: myco.bay]
    #27521967 - 10/28/21 06:51 PM (2 years, 2 months ago)

Pre made pan substrate


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[


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InvisibleModularMind
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27522231 - 10/28/21 10:52 PM (2 years, 2 months ago)

Coy, eh?


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Invisiblecoversall
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27522421 - 10/29/21 04:03 AM (2 years, 2 months ago)

Quote:

Jakeoncid419 said:





wooahhhhh


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


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Invisiblecoversall
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Re: Official Copelandia/Panaeolus Thread [Re: PitcherCrab]
    #27522422 - 10/29/21 04:05 AM (2 years, 2 months ago)

Quote:

PitcherCrab said:
Some more Peace River and Nec-D pics






Nice! Man I would love some of those ziploc pots, they look awesome.

Do you just unscrew the lid a little for GE?


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: coversall] * 3
    #27522549 - 10/29/21 08:17 AM (2 years, 2 months ago)

How's it going, everybody. Lots of new faces on the thread. Looks like
you guys are all just killing it, too. Very nice to see all the good
work going on here.

I am running two clones of the Australian Hunter Valley, "Big" and "Pretty"
but both are just pretty big :lol:



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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #27522563 - 10/29/21 08:38 AM (2 years, 2 months ago)

WOAH those things are huge Asura! Love the massive bell-shaped cap on the normal size stem.

Would love to get ahold of those once I can get my current grow attempts sorted out (some of us aren't killing it you know lol).



I cased 7 of my trays today since they looked 100% consolidated and put them in my tent. I am really, REALLY hoping these don't all contam out on me as has happened in all but one previous attempt. I've changed a few things so hoping the changes are positive. I also loaded up 7 more jars to keep the momentum up whether these trays succeed or fail.



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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27522619 - 10/29/21 09:39 AM (2 years, 2 months ago)

Hindsight, are you spawning directly to trays? I saw that pic up there and it kind of looks like it.
If so, you will have better luck growing it out in bags for 10 days or so, then breaking it up
and putting it in a tray. You let that sit for about 5 days and then fruit.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #27522649 - 10/29/21 10:07 AM (2 years, 2 months ago)

Thanks for the advice Asura - When you say spawning directly to trays, you are saying taking fully consolidated grain jars, mixing them with substrate, and then tamping that down into trays and putting a clear plastic cover over them while they consolidate? If so, yes that is what I have been doing.

I actually tried your method before any other method and it was an epic failure but all because I didn't have a tall enough SAB, I didn't have the right size needles and syringes, my hot glue gun I used to try to seal the needle hold ended up melting the grow bag... I could go on and on but I'm sure you're laughing hard enough already. Even I was laughing at what a disaster it was. That was quite a while back though and I now have all the tools and supplies I need to work with bags properly so I have been wanting to give it another go. My only fear is that it's difficult to properly mix consolidated grain spawn in a myco bag. But next week once I have some more grain jars ready, I'm going to give it a go - I'll try a bag with grain spawn and I'll try another with LC that will be ready by then.


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27522665 - 10/29/21 10:29 AM (2 years, 2 months ago)

Forget all the LC stuff. I haven't used that method in a year.

Just pasteurize enough sub for 1 tray in each bag. Add grain spawn to the bag, mix
it up, seal, let it grow out for 10 days or so (you can mix it up again at day 5).
When the bag is grown out, break it up and put it to a tray and cover for 5 days or so.
Case and fruit.

I think you will see a lot less contamination if you go this route.


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