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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: nosf3r4tu]
    #27507888 - 10/17/21 12:14 PM (2 years, 3 months ago)

Bubble wrap is a great idea! Cheap, widely available, and it won't block the light!


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: nosf3r4tu] * 1
    #27507894 - 10/17/21 12:19 PM (2 years, 3 months ago)

Gulf coast Florida pinning on a plate:



A while back I mentioned I was having a lot of issues with grain jars. Got a lot of good advice from everyone thank you. Most of the jars I shook never came back to life. I know most people say that means it is contaminated but it really didn't look like that to me so taking inspiration from a post Josex made a while back, I copied the idea and injected some sterilized water into one of the grain jars that refused to come back to life after shaking. I shook it up, let it rest a half hour, then pulled some of the water back out of the grain jar with a fresh sterile syringe. I looked at a drop under the microscope and saw no bacteria. So I put some of the water on a fresh agar plate and let it incubate 3-4 days. Zero bacterial growth. I think the issue might be my grains are too dry.

Another thing is that I've never used millet before. I asked for millet at the feed store and got a bag, but days later (after I'd used it) noticed the small tag on the bottom that said "milo" lol. So I'd been using  milo thinking it was millet. I got the right stuff but think my first attempt was too dry:



vs following Phu's millet tek and getting this. You can see how many more burst grains there are with his tek vs my prior attempt. I know you don't want tons of burst grains but I think having close to 0 is just too dry:


I should know in a week or so how the pans like it.


What do you guys think of this plate? The mycelium pattern is a bit strange looking to me. This look normal to you? The pattern is almost cobweb-mold looking, but it's too dense and too slow growing to be cobweb.


vs this is what most of my pans look like:


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27508054 - 10/17/21 02:43 PM (2 years, 3 months ago)

Hindsight, depends on species and agar recipe. Are both plates the same species?


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27508059 - 10/17/21 02:46 PM (2 years, 3 months ago)

:whathesaid:
The mycelium on your first plate looks unusual, at least for my eye, looks like my semilanceata plates
but that doesn't mean that it's no good.


Don't have much time for mush work at the moment but still fiddling around with a few clones, after thinning out
plates I ended up with 5 individual clones that I'm cleaning up a bit more, will take them to T3 before using as
I had a couple where bacteria made it over to T1.

One pin I had on agar got transferred to a new plate cause it was showing very good recovery growth but was hopelessly
surrounded by bacterial goo. Just had a look at it to check if it outgrew the contam and found it was pinning directly
from the clone tissue. That's the first time seeing my cambo pinning on a plate :lol:



Edited by Baba Yaga (10/17/21 02:56 PM)


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27508093 - 10/17/21 03:10 PM (2 years, 3 months ago)

Thanks guys. Those two plates are different varieties of pans. I put both to grain today so let’s see what happens.


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OfflinePitcherCrab
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #27508177 - 10/17/21 04:17 PM (2 years, 3 months ago)

Quote:

ruawakeyet said:
Bubble wrap is a great idea! Cheap, widely available, and it won't block the light!




I had the thought that I could insulate the FC… or even put it into a standup cabinet and heat the inside of the cabinet vs heating the tub using the fish tank heater. I’m not sure the solution, but I feel like I’m drawing closer to something. Alternatively if I just allow the condensation to happen I wonder if I can get a sheet of something very slick like a sheet of PTFE and then put that at an angle so the water slides off it more easily.

:hmm:


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Offlinerockyfungus
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27508182 - 10/17/21 04:18 PM (2 years, 3 months ago)

Just took the last of my pans, 2gs. That was intense.

About ready to get wild coast to grain and also some verm/compost cakes. Anyone messed with good black compost?


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus]
    #27508238 - 10/17/21 05:19 PM (2 years, 3 months ago)

You mean like store bought? Like black kow? If so I’ve used it for cubes with success.


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Offlinebagga
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27508341 - 10/17/21 06:56 PM (2 years, 3 months ago)

Hey pan fam,

Forgive the simple question, but with JOCs substrate recipe is the straw poop ratio by weight or volume? I couldn't tell from search

I just hand chopped a ton of straw, and I realized I don't know how to measure the quantity for the sub :smile:


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: bagga]
    #27508360 - 10/17/21 07:22 PM (2 years, 3 months ago)

I think his latest is equal parts straw and poo by volume.


Edited by ruawakeyet (10/17/21 07:33 PM)


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Offlinerockyfungus
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27508417 - 10/17/21 08:07 PM (2 years, 3 months ago)

Quote:

Hindsight said:
You mean like store bought? Like black kow? If so I’ve used it for cubes with success.




Yeah black kow is SHIT and will work. I'm talking good old fashioned compost (manure, browns, greens, mush substrate, etc)


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Offlinebagga
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #27508435 - 10/17/21 08:23 PM (2 years, 3 months ago)

Awesome, thanks!


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: bagga]
    #27508468 - 10/17/21 08:49 PM (2 years, 3 months ago)

Bagga - I saw he said it wasn’t by weight or volume when he was asked that - just approximate 65/30.

Ruawakeyet - where did you see that? I missed it. I need to cook up a batch tomorrow.

Rockyfungus - Yeah it’s not great but it is the only black compost readily available to many people. I have only grown a few tubs with it and it underperformed vs coir but it did actually produce much bigger fruits, though fewer of them and I only got one flush. Outdoors is where it shines. My outdoor cube bed is fully colonized grain jars dumped onto the ground then covered in a thin layer of black kow right out of the bag. The outdoor bed produced some massive caps - much bigger than my indoor tubs. Haven’t tried it with pans.

Maybe next season I’ll build a compost heap and see how well it works with pans and cubes.


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Offlinebagga
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27508499 - 10/17/21 09:09 PM (2 years, 3 months ago)

Uh.. ok. How are you making your subs?

I usually weigh everything, I don't tend to improvise 🤣😅... so I'm not sure how to get the ratio right 'cause density of straw and poop is so different.

Do you just kinda eye ball it and add water?

Like 2qt poop to 3qt straw? Verm to taste..


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Edited by bagga (10/17/21 09:10 PM)


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: bagga]
    #27508524 - 10/17/21 09:40 PM (2 years, 3 months ago)

I had the same question. All I know is a couple people asked and were told neither weight nor volume - just approximate. I struggle with that, but I tried doing it by weight and the manure content was way too low (visually). I ended up doing full trays composed of 100g dry straw, 1 cup dried manure, and 2/3 cup verm.

I’m very new to pans though so hopefully some others will chime in.


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27508546 - 10/17/21 10:04 PM (2 years, 3 months ago)

This is from JOC TEK, page 24: (https://www.shroomery.org/forums/showflat.php/Number/25925194/fpart/24/vc/1)

"Volume however as my material changes I may tweak it a little bit but that’s just to give you a ballpark idea if my pool is more dense than I may cut in more straw to soften it up, But yeah basically if I take a 5 gallon bucket and fill it up about a third with manure then add chop straw till it’s 3/4 of the way full mix it up really well add a little bit of vermiculite about a quart jar worth mix it again then add water to bring to field capacity. If my manure is really light and fluffy I may go a little heavier with it but I don’t have any hard evidence that doing so increases your yield"


I don't remember where I saw him mention going to equal parts straw and poo, (verm is still used, but I'm not sure how much.) It's possible it wasn't him, after 1,100 pages plus reading everyone else's write-ups, it all gets kind of mixed up.

On page 17 of JOC PAN TEK, a user went by weight instead of volume, and it colonized in 2 days, but I never saw pictures of fruit from that user.

I can say that 3 pints of compact, chopped straw only weighs around 100 grams dry, and 2 pints of compact, broken up hpoo weighs in around 140 grams. When you add that to 1 cup of vermiculite (30 grams), plus 1/4 cup of gypsum (58 grams - JOC said he doesn't even use gypsum anymore), plus 1 myco pint of spawn is enough to fill one 3 qt pyrex cake pan right to the top.


Edited by ruawakeyet (10/17/21 10:30 PM)


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OfflinePhosCap
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Re: Official Copelandia/Panaeolus Thread [Re: PitcherCrab]
    #27508568 - 10/17/21 11:14 PM (2 years, 3 months ago)

Quote:

rockyfungus said:
Just took the last of my pans, 2gs. That was intense.

About ready to get wild coast to grain and also some verm/compost cakes. Anyone messed with good black compost?



Quote:

PitcherCrab said:
Quote:

ruawakeyet said:
Bubble wrap is a great idea! Cheap, widely available, and it won't block the light!




I had the thought that I could insulate the FC… or even put it into a standup cabinet and heat the inside of the cabinet vs heating the tub using the fish tank heater. I’m not sure the solution, but I feel like I’m drawing closer to something. Alternatively if I just allow the condensation to happen I wonder if I can get a sheet of something very slick like a sheet of PTFE and then put that at an angle so the water slides off it more easily.

:hmm:




I know what you mean by mega condensation. All depending on ambient temps I noticed my tubs were under and over condensation. I ran a hepa filter next to them and they would typically seem dryer on the lids. Also ran my ceiling fan.
Eventually i turned all fans off because I don't think they were necessary. The Pans grew regardless. I just tilted the lids sometimes, propped them up usually 80% of the time. I neglected my pans like i did with my cubes and they all grew out fine.:lol:
One thing i did do daily was purge the condensation on a towel or bucket on the floor. Make sure to lift lid straight up. as soon as you tilt all of it will flow off.


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OfflinePitcherCrab
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Re: Official Copelandia/Panaeolus Thread [Re: PhosCap]
    #27508583 - 10/17/21 11:46 PM (2 years, 3 months ago)

Gotcha! Maybe I'm just overthinking it! Do you have holes drilled in your kids? I'll start purging the water a couple times a day and see if that improves things. I still see some mycelium growing under the casing layer so perhaps they're doing fine.

I was thinking I may try getting a flat piece of plexiglass and putting that at an angle on top. Drops should flow down that more easily than the standard tub lid.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #27508735 - 10/18/21 07:43 AM (2 years, 3 months ago)

Quote:

ruawakeyet said:
This is from JOC TEK, page 24: (https://www.shroomery.org/forums/showflat.php/Number/25925194/fpart/24/vc/1)

"Volume however as my material changes I may tweak it a little bit but that’s just to give you a ballpark idea if my pool is more dense than I may cut in more straw to soften it up, But yeah basically if I take a 5 gallon bucket and fill it up about a third with manure then add chop straw till it’s 3/4 of the way full mix it up really well add a little bit of vermiculite about a quart jar worth mix it again then add water to bring to field capacity. If my manure is really light and fluffy I may go a little heavier with it but I don’t have any hard evidence that doing so increases your yield"


I don't remember where I saw him mention going to equal parts straw and poo, (verm is still used, but I'm not sure how much.) It's possible it wasn't him, after 1,100 pages plus reading everyone else's write-ups, it all gets kind of mixed up.

On page 17 of JOC PAN TEK, a user went by weight instead of volume, and it colonized in 2 days, but I never saw pictures of fruit from that user.

I can say that 3 pints of compact, chopped straw only weighs around 100 grams dry, and 2 pints of compact, broken up hpoo weighs in around 140 grams. When you add that to 1 cup of vermiculite (30 grams), plus 1/4 cup of gypsum (58 grams - JOC said he doesn't even use gypsum anymore), plus 1 myco pint of spawn is enough to fill one 3 qt pyrex cake pan right to the top.




Thanks, I will play around with the ratios as well and see what works best for me.


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27508839 - 10/18/21 09:30 AM (2 years, 3 months ago)

My Bisporus N2 culture just got cased. I'm having high hopes, the previous Bisp culture didn't shrink the substrate, and this one does, quite well. Not as extremely as Cyans, but pretty good. Also I saw some primordia on the substrate even before I cased it.

Should be a good culture, thanks a lot to Chocolate Milk for sharing! The Rainbow Eucalyptus will be spawned soon too.


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