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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: moonwatcher]
#27496643 - 10/07/21 09:03 PM (2 years, 3 months ago) |
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Hi moonwatcher.
It's hard to say if your cultures are clean or not, as myc changes it's growth characteristics depending on the media it's growing on. I've never seen that media used before, so in case no one else answers your question, I would suggest to transfer a sector to some grain and see what happens. Don't do it in open air though, use what you have to keep it sterile.
I guess you could also transfer to bulk to see what happens, just don't plan on it taking off quickly with just a wedge to inoculate it.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#27497198 - 10/08/21 10:03 AM (2 years, 3 months ago) |
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Second tray finally producing its first flush. These fruits are thicker stemmed, larger cap diameter, and shorter than my first tray, even though all four of my trays were grown from the same clone.

I have two more trays that are just starting to produce pins but after that, it may be a long time before I can get any more pan fruits because I haven't been able to successfully colonize a grain jar in over a month. I mentioned a while back that I got a bunch of spore prints of different pan varieties (NecD, Alabama, BVI, etc) and was looking forward to seeing if the only pan variety I had previously been working with was the cause of my finicky jar results but clearly that's not the case and I am doing something wrong because I can't get ANY of the pan varieties to work and they all do the same thing: Grow super slow and/or stall out in the jar. The jars in the photo below are all 7 days old, and are of 3 different varieties. They are moving, but very, very slowly. As soon as my next agar plates mature, I will try the grain prep method JOC's just described to me.

I also got a 1000x microscope setup this week! I've just started dialing it in and am excited to use it to help ID things I find out in the wild, and also to check plates and grain jars for contamination to see if that might be the cause of my issues. For example, I've shown some pics of some plates that look odd to me, but there's no telling for sure if they are healthy or contaminated so I plan to biopsy them and check them under the microscope to see. The only problem is that I'm new to mycology microscopy and I haven't been able to find many example photos to help me differentiate between healthy mushroom mycelium and molds and other contams. I'll spend more time searching.
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27497482 - 10/08/21 03:12 PM (2 years, 3 months ago) |
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I have noticed slow or poor results if I leave the agar wedge on top of grain. To combat this, I first shake and tap the jar until the grains are free flowing. Usually they are kind of stuck to each other after cooling down from the pressure cooker.
Once the wedge or wedges are transferred and the lid is back on, I roll and gently shake the jar until the wedge or wedges are worked to a side and or near the bottom. This can help cultures jump on to the grain faster, then once I see it grow out a little bit, I shake everything up. I am still getting wedges stuck to the glass sometimes, but it still works.
Getting that microscope is awesome! I want to get one someday.
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Baba Yaga
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Registered: 09/13/20
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27497495 - 10/08/21 03:25 PM (2 years, 3 months ago) |
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Quote:
Hindsight said:
I have two more trays that are just starting to produce pins but after that, it may be a long time before I can get any more pan fruits because I haven't been able to successfully colonize a grain jar in over a month. I mentioned a while back that I got a bunch of spore prints of different pan varieties (NecD, Alabama, BVI, etc) and was looking forward to seeing if the only pan variety I had previously been working with was the cause of my finicky jar results but clearly that's not the case and I am doing something wrong because I can't get ANY of the pan varieties to work and they all do the same thing: Grow super slow and/or stall out in the jar. The jars in the photo below are all 7 days old, and are of 3 different varieties. They are moving, but very, very slowly. As soon as my next agar plates mature, I will try the grain prep method JOC's just described to me.

Does this problem still persist after a shake? I would try and give those jars a shake, mine doing the same but a shake usually jump starts more vigorous colonization.
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#27497511 - 10/08/21 03:41 PM (2 years, 3 months ago) |
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Hindsight, for 7 days old those jars look fine to me. Don't overthink it. My Rainbow Eucalyptus was extremely fast on agar, but once I dropped it into a jar it slowed down considerably. Yet it never occured to me to freak out about it. On the grain the speed is very similar to my Bunnell FL Cyans, so all is good in my book. Just be patient and you'll be surprised!
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27497604 - 10/08/21 04:53 PM (2 years, 3 months ago) |
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Thank you all three for the replies. I have not shaken these and I have avoided shaking pans - partly out of superstition because I've read they don't like it so much and I've also had bad experiences shaking the jars and then having them stall out completely. But I'm starting to think that it has less to do with my shaking and more to do with the fact that they were going to stall out anyway. When I was growing cubes, I would always shake at 25% and then again around 80% or so and had fantastic results with doing that.
Adas - Do you shake yours?
ruawakeyet - I did try that on a few jars and it seemed to really p*ss of the mycelium, which then never even grew off the wedge. It could be my jars are too wet or too dry or maybe my seed is coated with something but I doubt the latter because I've tried three different grain types with more or less the same result.
I will go ahead and shake half of them and see what happens. Thanks again for the input!
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Fridgedoor
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 2
#27498078 - 10/09/21 12:31 AM (2 years, 3 months ago) |
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Quote:
Baba Yaga said:
Quote:
Fridgedoor said: Hi, I got a quick question on my Pan grain jars. I tried the search function but got different answers.
Is it advisable to shake pan jars? I figured that the mycelium is more fragile/doesn't recover as quickly as cube mycelium. These jars take their sweet time...

Thank you in advance for any guidance.
Quote:
Baba Yaga said: you can shake just like any other mushroom IMHO
can post a photo of my jars I shook three days ago tomorrow
Just to follow up, here is a photo of a pan cambo jar 5 days after I gave it a shake

Thanks for the follow up man! Now, I feel that I need to do a follow up too . 3 days after shake and it seems to recover nicely (also it seems to be growing even faster)
-------------------- Hokus Pokus Fidibus!
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gt40
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Re: Official Copelandia/Panaeolus Thread [Re: Fridgedoor] 3
#27498106 - 10/09/21 01:20 AM (2 years, 3 months ago) |
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cloning the piece of australian red spore cap
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: gt40]
#27498250 - 10/09/21 07:00 AM (2 years, 3 months ago) |
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What do you have growing in that planter gt40?
So I'm thinking maybe the strange growth I was seeing on my plates was caused by the agar formula I was using, and not some kind of mold/contamination. I quit trying to transfer from it to the same agar formula and started transferring to grain water agar and immediately the grow looks very normal to me. The plate on the right has the odd looking growth. Ignore the two spots at 9:00 and 10:00 which were where I took my transfers from a few days ago, but do note the solid white growth pattern and the speckled areas that form a partial ring around the center. The plate on the left is grain water agar and is from a transfer I took from the plate on the right. The plate on the left looks good to me. I'm going to stick with low-nutrient agar formulas for a while until I can get things looking consistent.
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Guerrilla
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Registered: 01/30/21
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Loc: United Kingdom
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27498352 - 10/09/21 08:46 AM (2 years, 3 months ago) |
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Quote:
Hindsight said: Thank you all three for the replies. I have not shaken these and I have avoided shaking pans - partly out of superstition because I've read they don't like it so much and I've also had bad experiences shaking the jars and then having them stall out completely. But I'm starting to think that it has less to do with my shaking and more to do with the fact that they were going to stall out anyway. When I was growing cubes, I would always shake at 25% and then again around 80% or so and had fantastic results with doing that.
Adas - Do you shake yours?
ruawakeyet - I did try that on a few jars and it seemed to really p*ss of the mycelium, which then never even grew off the wedge. It could be my jars are too wet or too dry or maybe my seed is coated with something but I doubt the latter because I've tried three different grain types with more or less the same result.
I will go ahead and shake half of them and see what happens. Thanks again for the input!
I shook pans jars a couple days ago and they had way less growth than any of your jars in the picture you posted.
Both recovering so well, and are gonna be finished much quicker than had I not. Shake them. You wonβt regret it.
-------------------- Being pissed on does not make you a real man.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Guerrilla]
#27498397 - 10/09/21 09:51 AM (2 years, 3 months ago) |
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Thanks. I shook two or three of them last night so will see what happens. When I shook them, I noticed that the mycelium that had colonized the grains was quite solid, which I know is not usually a good sign in cubes (often sign of bacterial contamination), but maybe pans are different. It did break apart though.
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27498473 - 10/09/21 11:16 AM (2 years, 3 months ago) |
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I always shake Pans. I have never experienced them not recovering. It's superstition or REALLY poor cultures IMO.
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A.k.a
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#27498481 - 10/09/21 11:23 AM (2 years, 3 months ago) |
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They seem to be super vulnerable to bacteria. I think a lot of people have shaken some mildly contaminated spawn and it never recovered and they thought it was the shake that did it.
Iβve definitely had a few pans jars that looked good but when I shook them they barely came back and started flooding with that light yellow metabolite liquid.
--------------------
LAGM2020     
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
#27498488 - 10/09/21 11:29 AM (2 years, 3 months ago) |
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I see, that's possible. I don't think I have really seen any bacteria in my jars since I bought an actual pressure cooker.
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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27498499 - 10/09/21 11:54 AM (2 years, 3 months ago) |
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Quote:
Hindsight said: What do you have growing in that planter gt40?
So I'm thinking maybe the strange growth I was seeing on my plates was caused by the agar formula I was using, and not some kind of mold/contamination. I quit trying to transfer from it to the same agar formula and started transferring to grain water agar and immediately the grow looks very normal to me. The plate on the right has the odd looking growth. Ignore the two spots at 9:00 and 10:00 which were where I took my transfers from a few days ago, but do note the solid white growth pattern and the speckled areas that form a partial ring around the center. The plate on the left is grain water agar and is from a transfer I took from the plate on the right. The plate on the left looks good to me. I'm going to stick with low-nutrient agar formulas for a while until I can get things looking consistent.

What agar recipe was it that you used?
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27498656 - 10/09/21 02:59 PM (2 years, 3 months ago) |
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Pan cyan bayou Magic
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#27498658 - 10/09/21 03:00 PM (2 years, 3 months ago) |
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-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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TheDoobsker
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419] 1
#27498800 - 10/09/21 05:39 PM (2 years, 3 months ago) |
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It's really odd to me that each flush of these Wild Coast is getting increasingly more full. Opposite of every tub of mushrooms I've grown.
  
I took some clones that are looking good so far. Usually with clones I'll make LC and store in the fridge in syringes. Any reason to not do that with pans?
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savan73
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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: Asura]
#27498886 - 10/09/21 07:30 PM (2 years, 3 months ago) |
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Post deleted by savan73
Reason for deletion: .
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: TheDoobsker]
#27499011 - 10/09/21 10:45 PM (2 years, 3 months ago) |
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Quote:
TheDoobsker said: I took some clones that are looking good so far. Usually with clones I'll make LC and store in the fridge in syringes. Any reason to not do that with pans?
Iβm curious about this as well - I feel like I have read pans donβt store well in the fridge - unlike cubes. There doesnβt seem to be much written about it though. I just inoculated a slant today with a pan clone, and I have LC jars grown out that I will try storing as well - all of it in a fridge. Iβll test after 1 week, 1 month, 3 months and 6 months and report back here .
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