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Adas
Lonely Dreamer



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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27472971 - 09/18/21 12:24 PM (2 years, 4 months ago) |
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I'd take from the bottom of the colony on the left. Prolly your best bet.
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27473204 - 09/18/21 04:33 PM (2 years, 4 months ago) |
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Those look good to me Smoothcat. Just like many of mine.
In other news - it looks like I finally got a tray that may give me some fruit!

But my last couple batches of grain jars appear to be stalled. They don't look contaminated - just stalled or at least super, super slow growing. I do see growth - it's growing at like 1/4" per day.
This is 9 days after knocking up with agar wedge. It's WBS. I traced that pink line yesterday so I could see how much it grew in 24 hours. As you can see, not much.

Same thing, after 9 days, only I shook this one a few days ago. Very slow going on the spread of mycelium:

These I did 4 days ago. I did JOC's grain formula of 75% WBS (mostly millett) and 25% rye berries, and I knocked them up with a G2G transfer from a nice looking oat jar (same oat jar I used for the tray shown above). You can see growth but it's slow and not really jumping off the oats very well.

I'm hoping it's just this variety. I have 14 plates of 7 different varieties germinating from spores so hopefully at least one of those will be really fast and more reliable.
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27473235 - 09/18/21 05:08 PM (2 years, 4 months ago) |
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Hindsight, what temperature are they colonizing at?
I know some say it doesn't matter, but I strongly prefer upper 70s even for colonization.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#27473238 - 09/18/21 05:10 PM (2 years, 4 months ago) |
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I have the room set on a heater to 77 degrees with a 1 degree differential so it stays between 76 and 77.
I have the room on a 12/12 light cycle.... I feel like some people like to colonize pans in the dark? Maybe I read that wrong. I thought all mycelium could benefit from light but most of what I read in the past was for cubes. I assumed it was the same for pans.
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27473354 - 09/18/21 06:47 PM (2 years, 4 months ago) |
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Well, the temperature isn't your problem, as that is perfect. I'm actually experimenting with allowing some light on colonizing jars for the first time right now. I haven't seen any difference compared to keeping them in the dark.
I can't see the tops of your lids, but I assume you have filtered gas exchange holes. I could see that slowing things down if there wasn't, but other than that, it might just be some bunk cultures that tend to stall.
Your grains look hydrated properly. I don't have enough experience to offer any other advice. I'll let others with more knowledge step in. 
Having so many cultures running will keep you busy but there's no other way to better play the odds.
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PhosCap
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 5
#27473355 - 09/18/21 06:51 PM (2 years, 4 months ago) |
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Finally cranked out some Pans throughout the last couple weeks. These are Pan Cyan Tasmania. Im running a bunch of JCM FC's and some low tech FC's. I had some over flow trays so decided to do some experimenting to get a feel for how Pan's grow. The Pan's growing in the low tech had temps about 74-70, no heater in room. Here is my results.
In this room I have a little hepa running with a little fan. Misted the inside of the dome everyday. The top of the dome holes are fully open.
This is prob the lowest tech you can get I stired the plastic bag ever so often to push fresh air in. Also made slight holes on all sides. Half of the bag is open at the top. This I believe is a one flush wonder tray.

One of 4 JCM FC's. Not a full canopy but Im very pleased with the results.
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Baba Yaga
♥ coir grower

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Re: Official Copelandia/Panaeolus Thread [Re: PhosCap]
#27473388 - 09/18/21 07:27 PM (2 years, 4 months ago) |
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Nice job PhosCap! I love the bag grow, now clone and repeat.....please
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#27473410 - 09/18/21 07:52 PM (2 years, 4 months ago) |
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Quote:
ruawakeyet said: I can't see the tops of your lids, but I assume you have filtered gas exchange holes.
On some of the jars I'm just running unmodified plastic lids. I've read they allow enough gas exchange to work fine. And on some other jars I'm running metal lids with rings, and I have two 1/4" holes punched in the lid with a full size SFD in place. I don't really see any difference between the two lid types personally.
I do think it may just come down to genetics. I can't wait to see what Alabama, NecD, and Subtropicalis do. The nice thing about growing those is that they are a known-quantity and if they don't perform for me, I know it's something I'm doing wrong, in which case I will continue making changes and running tests.
Speaking of tests, I did one bag of substrate using the InstaPot 12 hour warm cycle pasteurization method, and for another bag I sterilized it in the PC for a few hours. I let both bags sit a week. The sterilized one smelled fine while the Instapot one stunk to high heaven. The instapot one is the one shown in my tray pic above so it looks to be working just fine though. I didn't use all of that pasteurized instapot substrate so just left the remaining quantity in the bag for 4 days or so and today it looks like it has some odd growth in it - almost looks like yeast.
PhosCap those are some big/thick pans! Nice work.
Edited by Hindsight (09/18/21 07:53 PM)
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PhosCap
Gratuitous Heavenly Grace



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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27473483 - 09/18/21 08:45 PM (2 years, 4 months ago) |
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Ive done bag growing with cubes. For some reason it really worked well for past projects. Im sure there are various mods you can do, like a miniature grow tent.
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A.k.a
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Re: Official Copelandia/Panaeolus Thread [Re: PhosCap]
#27474028 - 09/19/21 07:45 AM (2 years, 4 months ago) |
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What’s your rh like in the room outside the bags/dome??
--------------------
LAGM2020     
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a] 1
#27474081 - 09/19/21 08:32 AM (2 years, 4 months ago) |
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My outdoor bed is starting to produce. All I did was dump a few colonized grain jars on the ground, cover with a thin layer of aged manure (not re-hydrated), then cover that with a thin layer of straw and water with a watering can. These fruits popped up about a week later and this should be just the beginning... most of these are around the edges.
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Guerrilla
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27474106 - 09/19/21 08:43 AM (2 years, 4 months ago) |
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Quote:
Hindsight said:

Quote:
Hindsight said: My outdoor bed is starting to produce. All I did was dump a few colonized grain jars on the ground, cover with a thin layer of aged manure (not re-hydrated), then cover that with a thin layer of straw and water with a watering can. These fruits popped up about a week later and this should be just the beginning... most of these are around the edges.
I plan to do the same soon, minus the straw. Saw a thread on it - crazy yields and only 3 days for an insane amount of shrooms.
-------------------- Being pissed on does not make you a real man.
Edited by Guerrilla (09/19/21 08:44 AM)
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Adas
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Re: Official Copelandia/Panaeolus Thread [Re: Guerrilla]
#27474160 - 09/19/21 09:13 AM (2 years, 4 months ago) |
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PhosCap, how the hell did the caps open up so beautifully with so little air exchange? Talking about the bag grows.
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Hindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Guerrilla] 1
#27474227 - 09/19/21 10:19 AM (2 years, 4 months ago) |
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Quote:
Guerrilla said: I plan to do the same soon, minus the straw. Saw a thread on it - crazy yields and only 3 days for an insane amount of shrooms.
Honestly I don’t think the straw is needed so your plan should work fine. Unlike indoor grows, I don’t even see the straw colonizing on the outdoor grow. It helps retain moisture in the manure and grains though.
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onethroughthree
Wanderer



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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27474255 - 09/19/21 10:46 AM (2 years, 4 months ago) |
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Hey guys. New to pan cyans. Lost a grow to trich about a month ago
Giving it another go, but I’m concerned with how this is looking. This brownish mycelium AND this big cloud white mycelium, can’t tell if either of them are a contam or not. I’m used to cube mycelium so any insight is helpful
Ms to agar to rye to hpoo. Colonized fully pretty quickly and did a very shallow quarter inch or so jiffy casing. The casing layer got overtaken within about 1-2 days. Sits in an sgfc and this is where we are now. What do you guys think...
YESTERDAY:



TODAY:

I have literally no idea at this point. Any help or input is appreciated
-------------------- Onethroughthree
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PhosCap
Gratuitous Heavenly Grace



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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
#27474298 - 09/19/21 11:17 AM (2 years, 4 months ago) |
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Quote:
A.k.a said: What’s your rh like in the room outside the bags/dome??
Around 50-60%
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Big_Dub
I'm just some guy



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Re: Official Copelandia/Panaeolus Thread [Re: PhosCap]
#27474309 - 09/19/21 11:34 AM (2 years, 4 months ago) |
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-------------------- split_by_nine said: click me you fuck do the right thing
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PhosCap
Gratuitous Heavenly Grace



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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27474323 - 09/19/21 11:42 AM (2 years, 4 months ago) |
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Quote:
Adas said: PhosCap, how the hell did the caps open up so beautifully with so little air exchange? Talking about the bag grows.
About 4 feet away im running a small desk fan pointing towards the end of the room, not directly on the bags. Approx 1/2-3/4 ft from the tray I have small holes, one on each side. Seems to be enough air transfer for these. I have a feeling if the holes weren't there they would have still grown out. Before they pinned I misted the casing and fanned twice. minimal work. I tried the bag tek a few years ago on cubes and grown them out almost better then the tubs. Seems to make a perfect micro climate for mushrooms. I should maybe start a main thread on this bag tek. Currently i have a cube tray and a couple pan cyan bali in some bags. we shall see if its consistent results
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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onethroughthree, Does your Jiffy mix contain coir? I was looking at Jiffy mix earlier this year and I noticed it contains coir. Coir is not recommended in the casing layer as it can be colonized more readily than peat moss and verm, it might be contributing to your overlay. (I think Jiffy mix used to be made from peat and verm, but they may have changed their formula since then, or it may be a regional thing.)
Did you perform any transfers on agar, or did you take directly from your germ plate?
Lastly, did you use any straw in your bulk, or was it only Hpoo?
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onethroughthree
Wanderer



Registered: 09/20/08
Posts: 168
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#27474864 - 09/19/21 07:36 PM (2 years, 4 months ago) |
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Quote:
ruawakeyet said: onethroughthree, Does your Jiffy mix contain coir? I was looking at Jiffy mix earlier this year and I noticed it contains coir. Coir is not recommended in the casing layer as it can be colonized more readily than peat moss and verm, it might be contributing to your overlay. (I think Jiffy mix used to be made from peat and verm, but they may have changed their formula since then, or it may be a regional thing.)
Did you perform any transfers on agar, or did you take directly from your germ plate?
Lastly, did you use any straw in your bulk, or was it only Hpoo?
Thanks for the reply So I saw a jiffy mix bag that had coir in it. I didn’t use that one. I got the other fully bright yellow bag. But I noticed it didn’t have any ingredients listed on the bag itself. Just assumed it was the right thing. I think going forward I’ll just do my own casing mix
I did several transfers until I had what seemed like a nice aggressive uniform growth on the plate. And the mycelium colonized very quickly in the jar
No straw in the bulk. Straight hpoo. Perhaps I shouldn’t have done that, I’m not sure. This is a real bummer...
I don’t really know what I should have done differently. Everything looked fine up until applying the casing which I obviously pasteurized. Those big white cloud balls were there before I cased, but I just assumed that was normal for pan cyan mycelium???
Next time I’ll try to follow a different tek that’s known for strong success. I don’t really want to do cakes tho
-------------------- Onethroughthree
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