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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: V.L]
    #27427947 - 08/14/21 12:57 PM (2 years, 5 months ago)

Second Pan attempt.

My first attempt was following the Tek where you sterilize mycobags that have a mix of manure, straw, and grains, then inject with LC. Neither bag reached full colonization. I didn't have the right syringe though, and dealing with mycobags and a huge syringe in a SAB was just awful.

So I went with the more traditional route. I had some clean plates from MS and nearly all of them pinned in the plate, to my surprise. I transferred the pins to new plates, grew those out, took a couple transfers off each of those and grew those out.

While the above was happening, I grew out some LC and some oats from clean agar samples. The oats did not colonize as fast as I would have liked (took about 30 days) but they look clean. The LC is definitely clean as I tested it on agar.

I'm waiting on dry aged manure so for now, I did 1:1:1 black kow, wheat straw pellets, and vermiculite (vermiculite because the black kow can get muddy when at field capacity). I made four 8x8 brownie pans, two of which I mixed the fully colonized grain spawn in and two of which I knocked up with 30cc of LC each.

Also built my SGFC which is what I will use until I am able to successfully fruit, then I may move to a humidifier setup but I'd really like to keep it simple. If the pan genetics I have will pin on the plate, then I would think they would do just fine in a SGFC.

Is there any recent consensus on using grain spawn vs just manure/straw knocked up with LC?



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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27428010 - 08/14/21 01:41 PM (2 years, 5 months ago)

I’ve ran both methods dozens of times and while they both work I have found that grains spawn has a higher overall success rate than LC, however some people prefer LC so it really comes down to personal preference but you should always start following a proven tech to the T then once you have success with that you can start making alterations to see if you can can improve on your problem areas


--------------------
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OfflineLieutenant Pan
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27428016 - 08/14/21 01:46 PM (2 years, 5 months ago)

I've failed at pans 3 times now.  Maybe 4th time is the charm.


--------------------
I never thanked ya fer saving my life.


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Offlinerockyfungus
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Re: Official Copelandia/Panaeolus Thread [Re: Lieutenant Pan]
    #27428078 - 08/14/21 02:25 PM (2 years, 5 months ago)

Where did you fail along the process?


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus] * 2
    #27428123 - 08/14/21 03:02 PM (2 years, 5 months ago)

you will likely have issues in a sgfc unless  ur able to hover over it and give it literally 24/7 attention. i know i tried it and it was a horrible mess and time consuming and in the end wasnt even close to practical. its a fine balance of having to have a lot of fae but yet still keeping a high rh while maintaining a temp of 77-80 deg f  its just an awful lot to maintain all those parameters in a monotub or an sgfc. just believe me as i played with it myself and got so frustrated i ended up buildn what i still use and thats linked in my sig along with pics and directions. but an automated set up like that or if u have the space for a proper greenhouse/martha is the much more practical way to go and will guve you greater chances at success


--------------------
PANAEOLUS FRUITING MADE SIMPLE



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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27428218 - 08/14/21 04:36 PM (2 years, 5 months ago)

Quote:

Jakeoncid419 said:
I’ve ran both methods dozens of times and while they both work I have found that grains spawn has a higher overall success rate than LC, however some people prefer LC so it really comes down to personal preference but you should always start following a proven tech to the T then once you have success with that you can start making alterations to see if you can can improve on your problem areas




Thank you! I have five more jars of grain growing now so am definitely headed down that path. And when I spawn them to bulk, I'll be using manure/straw instead of black kow and straw - so following the teks more to a T.


Quote:

jcm4620 said:
you will likely have issues in a sgfc unless  ur able to hover over it and give it literally 24/7 attention. i know i tried it and it was a horrible mess and time consuming and in the end wasnt even close to practical. its a fine balance of having to have a lot of fae but yet still keeping a high rh while maintaining a temp of 77-80 deg f  its just an awful lot to maintain all those parameters in a monotub or an sgfc. just believe me as i played with it myself and got so frustrated i ended up buildn what i still use and thats linked in my sig along with pics and directions. but an automated set up like that or if u have the space for a proper greenhouse/martha is the much more practical way to go and will guve you greater chances at success




Thanks for the reply - my hope with the SGFC was that all of the variables would be handled automatically by design of the SGFC. I keep my room at a constant 45% humidity via whole house humidifier, and I have temp control to maintain a steady 76F. So those two variables are locked-in. I thought an SGFC got you enough FAE and enough humidity by way of design so it would be fairly hands-off. I'm a tinkerer/engineer at heart and have been deliberately holding-off on buying a bunch of tubs and hoses and atomizers and fans and cycle timers (even though I want to get into all that) in hopes that I can just keep it simple with the SGFC.

I've bookmarked your sig link and will give it a read this weekend to get some more ideas. Thank you!


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Offlinekarri0n
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27428229 - 08/14/21 04:47 PM (2 years, 5 months ago)

Is there anywhere to really reliably get hpoo for an ok price other than knocking on doors of farms or stables?


--------------------

Panaeolus Bisporus


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OfflinePhosCap
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27428232 - 08/14/21 04:48 PM (2 years, 5 months ago)

Quote:

Jakeoncid419 said:
How old are they? they don’t look like contamination but if your culture is moving super slow then there is something wrong. those dishes look like they have a super high nutrient profile I would suggest reducing it to get your culture to reach out across the dish




My dishes do have the recommended agar:nute ratio One dish seems like its somewhat normal, then there's two dishes that looks like no myc on the dish itself but you can see yes it did explode from the center and escaping the the dish itself and now entering the Pan Bali plate next to it. The myc or mold knows theres shit to eat across the other side.


--------------------
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OfflineV.L
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Re: Official Copelandia/Panaeolus Thread [Re: karri0n] * 1
    #27428251 - 08/14/21 05:00 PM (2 years, 5 months ago)

H poo is free from the ass of those big animals but if you can’t find it easily and close from your location, you can check in gardening shops, sold as fertilizer (Hpoo, cow poo, even see but not tried as dehydrated pellets in some places)


Edited by V.L (08/14/21 05:03 PM)


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Offlinekarri0n
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Re: Official Copelandia/Panaeolus Thread [Re: V.L]
    #27428271 - 08/14/21 05:20 PM (2 years, 5 months ago)

Cow poo is easy to get. People say hpoo is superior, but any garden centers I've been to don't carry it. I've been to several over the years, and people are nosy round these parts so it's not super fun asking around.


--------------------

Panaeolus Bisporus


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OfflineV.L
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Re: Official Copelandia/Panaeolus Thread [Re: karri0n]
    #27428279 - 08/14/21 05:33 PM (2 years, 5 months ago)

Cow poop is often to wet but mixed with straw can work too, even some rich organic potting soil can do the trick if you really can’t find Hpoo

Thanks learned the word nosy!


Edited by V.L (08/14/21 05:36 PM)


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27428401 - 08/14/21 07:37 PM (2 years, 5 months ago)

Quote:

Hindsight said:
Second Pan attempt.

My first attempt was following the Tek where you sterilize mycobags that have a mix of manure, straw, and grains, then inject with LC. Neither bag reached full colonization. I didn't have the right syringe though, and dealing with mycobags and a huge syringe in a SAB was just awful.

So I went with the more traditional route. I had some clean plates from MS and nearly all of them pinned in the plate, to my surprise. I transferred the pins to new plates, grew those out, took a couple transfers off each of those and grew those out.

While the above was happening, I grew out some LC and some oats from clean agar samples. The oats did not colonize as fast as I would have liked (took about 30 days) but they look clean. The LC is definitely clean as I tested it on agar.

I'm waiting on dry aged manure so for now, I did 1:1:1 black kow, wheat straw pellets, and vermiculite (vermiculite because the black kow can get muddy when at field capacity). I made four 8x8 brownie pans, two of which I mixed the fully colonized grain spawn in and two of which I knocked up with 30cc of LC each.

Also built my SGFC which is what I will use until I am able to successfully fruit, then I may move to a humidifier setup but I'd really like to keep it simple. If the pan genetics I have will pin on the plate, then I would think they would do just fine in a SGFC.

Is there any recent consensus on using grain spawn vs just manure/straw knocked up with LC?








If you like low tech then try a monotub as well, they work well for me without constant attention, I actually just mist for the first 3 days and after that
it's no maintenance at all, link is in my sig. You can always move on to the more evolved FCs later.


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Offlinekarri0n
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27428439 - 08/14/21 07:55 PM (2 years, 5 months ago)

If the reason for hpoo is just the consistency then I'm a lot less worried about it. I throw straw through a coffee grinder and mix with black Kow, verm, and oat flour. Sprinkle some gypsum.

It's definitely not like mud. Fluffy and I can even shake it, not as well as grains but still.


--------------------

Panaeolus Bisporus


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Invisiblecoversall
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Re: Official Copelandia/Panaeolus Thread [Re: karri0n]
    #27428688 - 08/15/21 03:01 AM (2 years, 5 months ago)

Fruiting pans in a SGFC sounds like a dice roll!


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..:: E V E R Y  ::..

..:: New? Start here. ::..
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OfflineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: coversall]
    #27428847 - 08/15/21 07:33 AM (2 years, 5 months ago)

I used composted steer manure with no problems. It’s like 2.75 for 50lbs at the nursery.


--------------------
LAGM2020


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Offlinerockyfungus
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #27428895 - 08/15/21 08:19 AM (2 years, 5 months ago)

Really no difference between cow and horse. Cow is just liquid and horse is ready to be shredded.

Horse and alpaca is readily available for me, no farmer asks questions they gotta pay to get rid of that SHIT and people want it for their gardens. If your lucky they'll get some farm equipment and scoop you a ton of SHIT.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus] * 1
    #27429013 - 08/15/21 10:20 AM (2 years, 5 months ago)

I notice a huge difference between grain fed cattle and grass fed cattle as for horse to cow it’s really not as important so long as whatever you choose is grass fed


--------------------
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EXOTICS
[


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OfflineV.L
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419] * 2
    #27430838 - 08/16/21 08:06 PM (2 years, 5 months ago)

Harvested few fruits mainwhile this one:




Printed that little bispo


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OfflineCocaineBuffet
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Re: Official Copelandia/Panaeolus Thread [Re: V.L]
    #27430852 - 08/16/21 08:19 PM (2 years, 5 months ago)

Nice looking fruits/veg. Those tomatoes look bomb. Is there a reason why you harvested some of the eggplant so early?

So I don't totally derail the thread: Is the main biological difference between horse and cow manure that horses are usually given grain?


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OfflineV.L
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Re: Official Copelandia/Panaeolus Thread [Re: CocaineBuffet] * 1
    #27431106 - 08/17/21 01:46 AM (2 years, 5 months ago)

Because I had a dinner..

Both horse and cow eat mostly grass naturally and make relatively good shit for our hobby (even cows have a 4 parts digestive system more effective with rumen to breakdown vegetal fibers from the grass) but nowadays lot of horses and cows aren’t in the field eating grass but are fed with supplemented food as grains and vegetal protein and those ones have less interesting poo for mycology that’s what I understand from Jakes comment..


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