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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Yeah I wanted to do a full sized mono or two minis but the LI did not work well. I make 5 jars and only two showed growth. The first one after two weeks and and second after like 3.5 or so. So I will do another 1 quart to 4 quarts or maybe make a ghetto FC out of some small container I find and do an1:1 or 1:2 in it.
Thanks again for the print man. I can't believe how all the blue specs after I shook the jars. And even on the wedges. One of them I did a wedge to grain. Nuts!!
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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That many quarts must make a deep substrate, what is the ideal depth for these beautiful little Pan ladies?
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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have you tried much cloning or work besides MS with pans mr.fox? I've always heard its not worth bothering with but I dont believe much of anything I've read about pans from old posts, very little seems to be true in my experience.
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: d0urd3n]
#23665874 - 09/21/16 06:02 PM (7 years, 4 months ago) |
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Quote:
d0urd3n said: Yeah I wanted to do a full sized mono or two minis but the LI did not work well. I make 5 jars and only two showed growth. The first one after two weeks and and second after like 3.5 or so. So I will do another 1 quart to 4 quarts or maybe make a ghetto FC out of some small container I find and do an1:1 or 1:2 in it.
Thanks again for the print man. I can't believe how all the blue specs after I shook the jars. And even on the wedges. One of them I did a wedge to grain. Nuts!!
Hah, yeah they bruise after shaking some times. I always go Agar->grain. I'll drop into 4 jars and turn each jar into 6-7 quarts. LI makes me kinda iffy. Hate not being able to visually diagnose my cultures
&& don't mention it, just giving back to the community.
-------------------- Giving is all we have, for we're just grateful to be alive

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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: spacechildo]
#23665881 - 09/21/16 06:08 PM (7 years, 4 months ago) |
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Quote:
spacechildo said: have you tried much cloning or work besides MS with pans mr.fox? I've always heard its not worth bothering with but I dont believe much of anything I've read about pans from old posts, very little seems to be true in my experience.
Yes I cloned my first Cambo grow. Had a hard time getting a sample from the stalk so I took it from the fleshy interior of the cap. I never was able to grow it out on a sub due to the fact tht I lost almost everything in the flood a few months back, but I believe cloning can bring about a more domesticated species amongst other things.. Plan on giving it another go in the next month or so.
-------------------- Giving is all we have, for we're just grateful to be alive

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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: Peteyboy]
#23665885 - 09/21/16 06:09 PM (7 years, 4 months ago) |
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Quote:
Peteyboy said: That many quarts must make a deep substrate, what is the ideal depth for these beautiful little Pan ladies?
They say nothing over 2" but I've done 5" easy
Like space said, alot of the old panaeolus info/faq have been debunked
-------------------- Giving is all we have, for we're just grateful to be alive

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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Quote:
Fantastic Mr. Fox said:
Quote:
spacechildo said: have you tried much cloning or work besides MS with pans mr.fox? I've always heard its not worth bothering with but I dont believe much of anything I've read about pans from old posts, very little seems to be true in my experience.
Yes I cloned my first Cambo grow. Had a hard time getting a sample from the stalk so I took it from the fleshy interior of the cap. I never was able to grow it out on a sub due to the fact tht I lost almost everything in the flood a few months back, but I believe cloning can bring about a more domesticated species amongst other things.. Plan on giving it another go in the next month or so.
oh man, I heard about that shit on the news, it seems to be happening a lot now in UK! I also heard the good shroomery people sent you a nice care package! hope you're on your feet again man, only ones who gets compensated for natural catastrophies around here seems to be the ones who's getting by fine anyway, us regulars get fucked!
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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I'm just getting into Pans, I have four jars of Goliath leaping off now, and just streaked a plate with some Sandose spores. Mr. Fox, Have you ever seen pan myc bruise and produce metabolites on agar?
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: spacechildo]
#23665948 - 09/21/16 06:35 PM (7 years, 4 months ago) |
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Quote:
spacechildo said:
Quote:
Fantastic Mr. Fox said:
Quote:
spacechildo said: have you tried much cloning or work besides MS with pans mr.fox? I've always heard its not worth bothering with but I dont believe much of anything I've read about pans from old posts, very little seems to be true in my experience.
Yes I cloned my first Cambo grow. Had a hard time getting a sample from the stalk so I took it from the fleshy interior of the cap. I never was able to grow it out on a sub due to the fact tht I lost almost everything in the flood a few months back, but I believe cloning can bring about a more domesticated species amongst other things.. Plan on giving it another go in the next month or so.
oh man, I heard about that shit on the news, it seems to be happening a lot now in UK! I also heard the good shroomery people sent you a nice care package! hope you're on your feet again man, only ones who gets compensated for natural catastrophies around here seems to be the ones who's getting by fine anyway, us regulars get fucked!
Yeah they did!! Thankful is an understatement for how I feel towards tht.  I've just recently got back at it, but bacteria is a mf. I've been trying everything from agar sandwich, peroxide, stiffer agar. It's like with some of these culture, PE esp. The bacteria is like embedded into the mycelial network. lol I was able to get clean catches from this Pan Vietnam print and a few others.. So I have tht in the works currently. Hopefully I'm able to propagate some prints to share. One thing I've noticed is this pan Vietnam culture is starting to throw out rhizomorphic growth rather than tomentose..kinda cool to see. I'll post some pice after they complete this run on the media.
-------------------- Giving is all we have, for we're just grateful to be alive

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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: Peteyboy]
#23665952 - 09/21/16 06:38 PM (7 years, 4 months ago) |
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Quote:
Peteyboy said: I'm just getting into Pans, I have four jars of Goliath leaping off now, and just streaked a plate with some Sandose spores. Mr. Fox, Have you ever seen pan myc bruise and produce metabolites on agar?
No I haven't experienced it bruising or putting out metabs on agar media. If it's doing all of tht I would say it's probably stressed due to some type of contamination. A picture is worth a thousand words though.
They've only bruised for me on agar after I've taken a transfer, thus stressing the culture to some extent. Dark Amber metabs are never a good sign.
-------------------- Giving is all we have, for we're just grateful to be alive

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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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The metabs were clear at first but they have turned amber now. I figured it was a contam but here's a pic I uploaded earlier...
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Official Copelandia/Panaeolus Thread [Re: Peteyboy]
#23666067 - 09/21/16 07:16 PM (7 years, 4 months ago) |
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I cant see any metabs, which would worry me more than bruised myc on agar, but its impossible for me to tell whats up with that pic. devil's in the details and I cant really see shit when its so out of focus
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: Peteyboy]
#23666094 - 09/21/16 07:24 PM (7 years, 4 months ago) |
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Quote:
Peteyboy said: The metabs were clear at first but they have turned amber now. I figured it was a contam but here's a pic I uploaded earlier...

I agree with, Space.. Looks sketch to me. I see clean mycelium but that blue/black spot is of a huge concern. Take a transfer or two onto a new dish and watch how it recovers/leaps off the wedge. Shouldn't take anymore than 24hrs for it to consume the wedge.
-------------------- Giving is all we have, for we're just grateful to be alive

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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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Yeah sorry about the crap pics, I tried for awhile bit couldn't get it to focus.
You can see the little droplets in the second pic better, they are sitting on the myc, looks like water on the glass. I made dropped the four dishes into grain a few hours ago. I had three nice plates and that one sketchy one. I sliced away from the bruise and threw that piece out. Made sure to mark the jar so I know that it could be contaminated
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Soreidorb
Builder


Registered: 07/31/15
Posts: 144
Last seen: 4 years, 3 months
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Re: Official Copelandia/Panaeolus Thread [Re: Peteyboy]
#23668110 - 09/22/16 01:28 PM (7 years, 4 months ago) |
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So despite overlay these beautiful creatures are pushing through
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Official Copelandia/Panaeolus Thread [Re: Soreidorb]
#23668141 - 09/22/16 01:43 PM (7 years, 4 months ago) |
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Good job brother 
Seems like I've taken a long break, gotta start out some new Pan cultures soon.
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Soreidorb
Builder


Registered: 07/31/15
Posts: 144
Last seen: 4 years, 3 months
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Re: Official Copelandia/Panaeolus Thread [Re: Teemo 6T3]
#23668183 - 09/22/16 01:56 PM (7 years, 4 months ago) |
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Thanks, I love cube grows but I'm loving the Pan process. I'm going to get some more Jamaicans on agar and probably try the Goliath as well.
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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Re: Official Copelandia/Panaeolus Thread [Re: Soreidorb]
#23668895 - 09/22/16 06:02 PM (7 years, 4 months ago) |
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This is a dish I streaked with cinctulus, looks like clean germination throughout.
I know i should tranfer but it all looks the same lol..what do you gusy think
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Official Copelandia/Panaeolus Thread [Re: Peteyboy]
#23668980 - 09/22/16 06:28 PM (7 years, 4 months ago) |
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I think you should transfer even if it seems clean throughout @ germination. Better safe than sorry
-------------------- Giving is all we have, for we're just grateful to be alive

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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: Official Copelandia/Panaeolus Thread [Re: Peteyboy]
#23668986 - 09/22/16 06:30 PM (7 years, 4 months ago) |
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I've never regretted taking an extra transfer personally 
Only will delay you by a couple days. And you will have peace of mind.
But I've nocced up jars without transferring so it's up to you 
That print from BL?
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