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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#28125779 - 01/05/23 01:07 PM (1 year, 22 days ago) |
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Got some Pan Cambo jars finishing up. Planning on spawning to shoeboxes and placing them under a cool mist humidifier. My substrate of choice is going to be black kow mixed with straw, verm, coir, and/or gypsum. Does anyone have a recipe for the bulk substrate? Exact amounts of black kow to water? I could not find anything definitive UTFSE.
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils]
#28221871 - 03/09/23 07:29 AM (10 months, 14 days ago) |
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I am planning on spawning pan cambo on grains to hpoo/coir. What ratio of hpoo to coir should I use?
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: ppancho]
#28264023 - 04/05/23 07:01 PM (9 months, 18 days ago) |
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Posted to the agar envy thread but nobody replied. All my pan plates grow very thick and pillowy before eventually stalling out. No other signs of contam ever show up. Any ideas? Enmeshed mold/bacteria? Best pics I can could manage to take. 1% LME agar.

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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 1
#28264097 - 04/05/23 07:35 PM (9 months, 18 days ago) |
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Appreciate the quick response. Using no pours and telephone brand 2g per 100 mL dH2O. Recently started lowering it to 1.7 g per 100 mL due to lack of germination. Always have to germinate on small pf tek cake first then transfer to agar. Will certainly try adjusting. Right now waiting for some transfers to grow out on water agar.
Edited by AspectOfTheCreator (04/05/23 07:45 PM)
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss] 1
#28272464 - 04/11/23 08:41 AM (9 months, 12 days ago) |
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Pan Cambo myc on water agar. Not as organized as I would have liked. This culture has been troublesome and I don't have anymore spores so trying to make this work.

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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] 3
#28278489 - 04/15/23 06:59 AM (9 months, 8 days ago) |
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I used producers pride oats. Going A2G, the pan myc downright refused to jump off onto the oats every single time. That same myc colonized wbs no problem.
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AspectOfTheCreator
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I rinsed 5x and boiled for ~35 minutes with tiny amount of gypsum. Watched very closely and removed from heat at the sight of the first burst grain. Strained 30 minutes, spread out, dried for 2 hrs. PCed for 2 hours at 15 psi. Grains were plump and dry on the outside. Freely flowing when the jar was tilted. No clumping or condensation. Regardless, no pan or cube myc would jump off from agar wedge onto the grains.
Even with clearly terribly prepared grains in the past and dirty agar, I would get SOME colonization. Like Vinny said, I never figured it out. Said the hell with it and moved onto different grains.
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
#28278962 - 04/15/23 01:02 PM (9 months, 8 days ago) |
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Streak from a spore print of BVI onto 0.75% LMEA. Germination or bacteria? Or still too early to tell? Let me know if more pics may help.
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: psilocybist]
#28279020 - 04/15/23 01:45 PM (9 months, 8 days ago) |
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Hard to get pics. Camera just wants to focus on the outside of the container.




Pretty sure it's myc. Just want confirmation.
Edited by AspectOfTheCreator (04/15/23 02:02 PM)
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
#28283284 - 04/18/23 07:14 AM (9 months, 5 days ago) |
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Quote:
PsiloPsychIn said: Hey Folks I bought a Pan Bisporus (Coville WA) spore syringe from a reputable vendor and the date on it is 10/22. There is plenty of dark spore looking stuff in the syringe. To my surprise the first attempt at germinating (2.5 weeks now) there has not been a wiff of germination of any thing. Other spores from prints that I inoculated that night have germinated a week ago. I mixed up a batch of super soft agar (about 8.5 grams of agar, 10 grams of Light Malt, and 500ml of water) and have squirted another batch of spore solution on there and have been waiting about a week. Nothing. Do Bisporus take a long while to germinate? Do they require something special to encourage them to germinate? I was hoping to have some MS mycelium to work with by now. I mixed up one more batch of agar (soft, 10 gm light malt, 1 gram nutritional yeast, and 3m grams activated charcoal. I'm hoping that might be the magic bullet. I'm excited to try these beautiful pans... but I'm impatient and also a bit anxious about the quality of these spores.
Depending on your agar that might not be soft enough. I use telephone brand and never managed to get reliable germination until I went down to 6.5 g agar per 500 mL H2O. As someone else said, try a BRF puck. As soon as you get germination transfer some myc to agar. Grows out very nicely like that.


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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss]
#28291582 - 04/23/23 02:53 PM (9 months, 9 hours ago) |
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Will pans pin on agar plates with no manure involved?
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: Gorguss]
#28293112 - 04/24/23 02:35 PM (8 months, 30 days ago) |
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My Pan plates always stall seemingly without reason. They never nake it to the edge of any plate. Any ideas? There's never any signs of contam as far as I can tell. Agar is 1.3 g agar and 0.75 g LME per 100 mL dH2O


Edited by AspectOfTheCreator (04/24/23 03:02 PM)
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28293470 - 04/24/23 06:00 PM (8 months, 30 days ago) |
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You touched on a good point I forgot to mention. If I take a transfer from this stalled plate, it will start growing without issue on the new one.
So you're saying I should just put cultures like this to grain anyway?
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 1
#28294259 - 04/25/23 06:18 AM (8 months, 29 days ago) |
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Appreciate the tip. Got a handful of regular plates going. Will update.
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#28296330 - 04/26/23 03:01 PM (8 months, 28 days ago) |
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Is this what I should be looking for? 1% LMA

I inoculated several pancakes. If I get even a single fruit from any of them, would it help to clone the fruit? I'm finding it nigh impossible to get a culture that doesn't at some point stall on agar.
Edited by AspectOfTheCreator (04/26/23 06:18 PM)
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: Sally_D]
#28301218 - 04/30/23 02:54 PM (8 months, 24 days ago) |
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Transfers taken from germ plate. This the type of growth I'm looking for? Kind of at a loss. All my pan plates stall out while they're still young on 1% LMEA. Actually just tossed one of those wedges into a grain jar just for the hell of it. Maybe what won't grow on agar WILL grow on grain? Really at a loss.
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: BirdsOnTrees]
#28301275 - 04/30/23 03:36 PM (8 months, 24 days ago) |
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They grow too thick on normal 2% LMEA from my experience so maybe lower nutritional content? Supposedly transfers past t2 are pointless and it's more about snagging the right myc from germination? Just what I have read. If I'm wrong please lmk.
It's really perplexing. If I transfer some stalled myc it will begin growing normally on the new plate for a little before stalling again. I shot MSS into pf tek cakes and they've colonized no problem. If I try placing some pf tek myc to agar it will grow well at first then stall out too. I feel like there's something fundamental I'm messing up or just not understanding because it doesn't make sense to me.
Edited by AspectOfTheCreator (04/30/23 04:59 PM)
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: esotericbound]
#28301441 - 04/30/23 06:07 PM (8 months, 24 days ago) |
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1 g LME and 1.4 g telephone agar per 100 mL distilled water. Was using 2 g telephone agar previously and had the same issues. Only difference is spores actually germinate and contam shows up better with 1.4 g telephone agar so I've stuck with that amount.
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: esotericbound]
#28301604 - 04/30/23 08:48 PM (8 months, 24 days ago) |
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I wrap my petris in glad wrap. I'm considering that maybe that's choking them out. Can't think of anything else.
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AspectOfTheCreator
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Re: Official Copelandia/Panaeolus Thread [Re: BirdsOnTrees]
#28301940 - 05/01/23 08:20 AM (8 months, 23 days ago) |
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Quote:
BirdsOnTrees said:
Quote:
AspectOfTheCreator said: I wrap my petris in glad wrap. I'm considering that maybe that's choking them out. Can't think of anything else.
I actually stopped wrapping my plates and started seeing much more vigorous growth on agar, especially with my pans, I didn't put these two things together but looking at my collection it's super obvious.
Seems so risky to not wrap up plates.
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