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ReverendMyc

Registered: 03/29/19
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 4
#28158124 - 01/26/23 10:24 AM (1 year, 1 day ago) |
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Congrats SirP.
I am with Shrimps and planning to start pans following Baba Ys lead. Thinking I want to start with bvi and bispo. Just wanted to get this tread in my list to easily follow along.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 4
#28180642 - 02/10/23 08:53 AM (11 months, 10 days ago) |
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Congrats Lefty!
I am in the pan game now.


Shredded a swab dipped in sterile water and put pieces to agar. This is day 6 for the germ plates. The BVI swabs came from a sponsor. Also got a spore syringe of bisporus from a different sponsor yesterday that went to agar last night.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
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Re: Official Copelandia/Panaeolus Thread [Re: ReverendMyc] 4
#28187100 - 02/14/23 12:52 PM (11 months, 6 days ago) |
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Our sponsors rule.
 BVI looking pretty clean for swab germination. Better than I am used to with swabs.
 And the syringe of Bisporus is showing some life after 4 days as well.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: Official Copelandia/Panaeolus Thread [Re: Seeker604] 1
#28208140 - 02/28/23 12:49 PM (10 months, 23 days ago) |
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Trying to keep my post count under 1000 until my international and non-machinable stamps arrive for a market place give away. But I would like some input on these T1s.
 BVI and Bisporus. First time with pans and planning to try a tub grow a la Baba Yaga. Anything to look for or try to avoid in pan myc selection? I see fast and whispy in the post above. These look pretty uniform in speed for each variety, but different from each other, and not necessarily whispy.
Agar recipe: 7g lme, 8g agar, 400 distilled water.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
Edited by ReverendMyc (02/28/23 12:54 PM)
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: Official Copelandia/Panaeolus Thread [Re: abductee] 2
#28244425 - 03/24/23 10:25 AM (9 months, 30 days ago) |
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I would appreciate some input on these. These are my first pans, ms BVI and Bispourus jars inoculated on 3/3 and shaken on 3/14.
Front and back of the BVI
Bispourus

I am not in love with the condensation on most and the poor recovery of the one bis 1129. That weak bis was the slowest on agar as well, so it is highly likely to fail.
But just finished the liquid poo pasteurization and coir prep (ty Baba Y) and have plenty for a mini-tub each. I have a mini-ez dial - water tub ready for each jar so no real harm in sending each. I am just looking for something to clone that does okay in tubs in my desert environment.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Nice work OFOW NGL, kind of jelly. Those are my first two flavors and no joy yet.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
Edited by ReverendMyc (05/26/23 09:44 AM)
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#28367236 - 06/20/23 01:22 PM (7 months, 4 days ago) |
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I didn't get any pan fruits on my first try. So I went back to spores. I am trying to clean these older plates up and put them to water agar. But so far, the mature plates look exactly like the first try. Sadly, the spawned trays that failed also looked like the same growth on these plates. They never fruited or showed obvious contamination.
So can more experienced eyes tell me if there is any hope for any of these or recommend anything?
Pan BVI
Looking at plate 1214 in the second picture you can kind of see some bluing that I saw on other fluffy mature plates and some of the trays. The blueing never turns to spores or expands past the inside of the thickest myc.
Pan Bisporus
 Do any of these donor plates look like they have hope or any other recommendations?
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
Edited by ReverendMyc (06/20/23 01:27 PM)
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 1
#28367380 - 06/20/23 03:07 PM (7 months, 4 days ago) |
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Thanks Yoshi
That is a big help. I think I see the pattern you do and will make some transfers accordingly.
BVI picture 2 and 3 are front and back lit of the same plate. All the rest are one backlit picture per plate.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
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Re: Official Copelandia/Panaeolus Thread [Re: SirPsycho] 1
#28370219 - 06/22/23 01:43 PM (7 months, 2 days ago) |
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GL sir
Quote:
SirPsycho said:


From your plate date, I thought you were from the future!
Then I remembered that everyone but me does dates wrong.
#yyyy-mm-dd gang
Okay, I will shut up now. I think I need to reduce my "micro-dose" a bit.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
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Re: Official Copelandia/Panaeolus Thread [Re: Newkoncept] 5
#28477089 - 09/20/23 08:25 PM (4 months, 5 days ago) |
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 Top fruting some bisprous quinoa half pints in hopes of something to clone. Never liked the look of the agar plates so this is a hail mary pass.
  This is a bisporus on 1 pint millet from a better looking plate a few transfers from the one above.
  And this is my best looking plate of bisporus to date heading to millet when it gets a little bigger.
Still fighting with BVI on agar.
  Kinda muffed the wedge transfer on this one.

-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
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Re: Official Copelandia/Panaeolus Thread [Re: Telomere] 2
#28477526 - 09/21/23 10:24 AM (4 months, 4 days ago) |
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I haven't found any particular advantage to using it. It is really expensive relative to other grains and seeds. I just had some on hand and wanted to test it. It works fine and I liked using a smaller grain than wheat and oats.
But that was before I found and tried millet. Now I am on team low prep millet. I can get a 50 pound bag of millet locally for $35. That is about the same as food grade wheat and twice what I pay for racing oats locally and 1000% worth it in time and ease of jar prep.
tldr; Eat your quinoa, use millet instead.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: Official Copelandia/Panaeolus Thread [Re: RoscoeReturns] 1
#28478530 - 09/22/23 10:05 AM (4 months, 3 days ago) |
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Thanks for that Roscoe! It gives me hope.
I am in the same boat as Genome with pans. @Genome794 In fact, a lot of your pictures could have come from my grows. So don't put too much weight in anything that I say. I am still trying to figure this species out myself.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: Official Copelandia/Panaeolus Thread [Re: Genome794]
#28479871 - 09/23/23 12:01 PM (4 months, 2 days ago) |
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Quote:
Genome794 said: I am willing to go one more round with these two plates...

Unless someone tells me they are more likely mold than Pan Cyan myc.
Thanks in advance.
EDIT: Nevermind... The Bisp of ReverendMyc above clears it up. My plates aren't nearly as organised.
Hey Genome, are you using a low nutrition recipe for pan plates? Dropping my lme down to 0.5% from 1.5 helped visually.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Dropping some solid knowledge! I am really enjoying and encouraged by your posts.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#28528014 - 11/03/23 03:07 PM (2 months, 23 days ago) |
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Not OP but FPJ is a wonderful rabbit/worm hole. Fermented Plant Juice. https://www.ijert.org/sustainable-hydroponics-using-fermented-plant-juice-nutrition Looks interesting.
I second the request for a thread to track you projects, kirkeng and sleepydave.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] 3
#28546262 - 11/17/23 09:35 AM (2 months, 9 days ago) |
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1st Thanks to a generous member I did some t1s from these last night. All of these germ plates are only 5 days old. Crazy fast.

Also did my first pan to water agar clone. I tried to do a traditional inner stipe flesh, which didn't go perfectly, but better than it could have considering the size. And I love how blue this fella turned within minutes of pulling apart.
 
And I am still amazed by the speed. Days from casing: 3,5,6,7,8
   
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
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Re: Official Copelandia/Panaeolus Thread [Re: TheAtlantean] 2
#28558877 - 11/27/23 09:07 PM (1 month, 30 days ago) |
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Also to Baba's comment.
This tub came from the middle jar in the second picture which is what it looked like two or three days after a shake. All three where shaken at the same time.
 
This was the two pan jars a day or two before the shake.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

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Re: Official Copelandia/Panaeolus Thread [Re: ReverendMyc] 12
#28558884 - 11/27/23 09:13 PM (1 month, 30 days ago) |
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Sorry for double post but I got excited about test results for BVI.
I expected the pans to be too potent for the test chart so I used half the amount per instructions from Miraculix (the maker of the potency test kits). So using 0.075 grams instead of 0.150 grams means you double the reading on the test.
   
I am reading 21mg/g x 2 for 4.2% actives. Tallest reading for me to date.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley]
#28559291 - 11/28/23 10:17 AM (1 month, 29 days ago) |
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Thanks all.
Quote:
theodor said:
Quote:
ReverendMyc said: ... I am reading 21mg/g x 2 for 4.2% actives. Tallest reading for me to date.
Nice! If that reading is accurate, you would have won the Psychedelic Club of Denver Mushroom competition. What scale are you using by the way? I'd like to get something similar.
https://www.amazon.com/gp/product/B0012TDNAM?th=1 I have several other cheaper and/or larger, but had to get this one for sufficient accuracy for testing at these levels. I use condiment cups on the plate to hold everything and it reliably detects the difference in their weights. I am happy with it.
Side note: I will be doing 1/3 tests with potentially potent exotics going forward. 0.050 grams and triple the reading will hopefully put them closer to the middle of the results color scale, which is generally preferable for extrapolation measurements.
And another side note: I sourced these spores from the place that the winner of one of the "Cups" claimed they got theirs. I fell for the hype, but it panned out this time.
Quote:
MojaveMyc said: As I experimented with other species, I said “fuck cubes” for sure but could never truly get rid of em. Nats, Tamps, Semps don’t give me deep introspective/therapeutic trips. Now that I’ve finally made my way to Pans, it’s fuck cubes fr 
I tried .25g TTBVI a couple weeks ago and had an intense experience. I could see up through my ceiling into the universe. Intricate OEVs with a brutal, dark headspace I’d blame on set/setting. Time dilation galore. I’d compare the experience to 1g strong/mutant cubes.
Thanks for this timely post Mojave. I have been really excited for these and was thinking, fuck it, I will do my standard 1g test dose for a new species. I Love, Love, Love lsd and visuals, so I am not a psychedelic novice. But I seem to have a pretty high sensitivity for mushrooms, in that they usually hit me pretty strongly. So I think I will be chicken and try a bit more moderation for the first go.
#teamfuckcubes
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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ReverendMyc

Registered: 03/29/19
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Re: Official Copelandia/Panaeolus Thread [Re: ReverendMyc] 1
#28559428 - 11/28/23 12:40 PM (1 month, 29 days ago) |
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Quote:
ReverendMyc said: Thanks all.
Quote:
theodor said:
Quote:
ReverendMyc said: ... I am reading 21mg/g x 2 for 4.2% actives. Tallest reading for me to date.
Nice! If that reading is accurate, you would have won the Psychedelic Club of Denver Mushroom competition. What scale are you using by the way? I'd like to get something similar.
https://www.amazon.com/gp/product/B0012TDNAM?th=1 I have several other cheaper and/or larger, but had to get this one for sufficient accuracy for testing at these levels. I use condiment cups on the plate to hold everything and it reliably detects the difference in their weights. I am happy with it.
Bad form to quote myself, but I just noticed the price drop to under $25 on that scale. Must be a black friday thing, cause I paid double that a month ago. Highly recommend this scale at this price.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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