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tree frog
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Re: Official Copelandia/Panaeolus Thread [Re: Genome794]
#28502844 - 10/13/23 06:56 AM (3 months, 14 days ago) |
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Was planning to wait until the new year but couldn't help it and streaked four plates of lme agar with pan. bisporus this morning.
Should I make some water agar for transfers? Or would grain water agar be better?
I've seen a lot of people are having issues with this stuff stalling, and I'd like to have the right plates ready for my first transfers.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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tree frog
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Re: Official Copelandia/Panaeolus Thread [Re: Telomere]
#28511608 - 10/20/23 10:51 AM (3 months, 6 days ago) |
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Quote:
Telomere said: I thought this Pan Bispo spore germ plate was looking pretty sexy. Took three transfers a few days ago and they are all super fast moving.
Thanks @thirdeyewild

I started four germ plates a week or so ago and just started four more this morning.
This is my first time with them so I appreciate the agar picture.
I have visible growth on one plate. It looks a lot like what you posted but a lot smaller. I haven't transferred it yet because it getminated towards the edge of the plate and I worry it's satellite mold because of the location.
If anyone has thoughts on what I should do. Right now I'm leaning towards waiting for more germination so I have a better idea what I'm looking at.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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tree frog
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Re: Official Copelandia/Panaeolus Thread [Re: Telomere]
#28511871 - 10/20/23 02:04 PM (3 months, 6 days ago) |
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Thanks. I'll give it a few days and see how it develops.
When people say grain water agar, is that from the soak or the boil? I made some grain water lcs yesterday from my rye berry boil for sclerotia and cut it with three parts water.
I think my millet soak water might be okay without dilution since it's not getting boiled. But maybe I should cut that 50/50?
Was planning to make some in the next few days.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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tree frog
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#28512010 - 10/20/23 03:34 PM (3 months, 6 days ago) |
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Here's the best photo without setting up the flowhood. First noticed it day before yesterday. Probably four times that size now.
On 2% LME. Pan. Bisporus. Nothing else noticable so far on any other plates.
I generally don't get satellites around my edges anymore but this looks like a place I cooled my inoculation loop before dropping spore solution on it. Rather than a place I streaked.
But solution could have ran into the crack.
Anyway, possibly mold.
?
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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tree frog
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
#28523164 - 10/30/23 09:13 AM (2 months, 27 days ago) |
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Quote:
tree frog said: Here's the best photo without setting up the flowhood. First noticed it day before yesterday. Probably four times that size now.
On 2% LME. Pan. Bisporus. Nothing else noticable so far on any other plates.
I generally don't get satellites around my edges anymore but this looks like a place I cooled my inoculation loop before dropping spore solution on it. Rather than a place I streaked.
But solution could have ran into the crack.
Anyway, possibly mold.
?
So I transferred this after letting it grow out a bit more and it seems to be doing well on both Water Agar and 1% LME. I dropped an MS wedge straight to millet too.
It does seem really slow on grain. Fast on agar.
I think I read some people have trouble with pans stalling on grains? Is this primarily an issue with MS or do I need to modify my grain prep somehow to get it to run faster?
Maybe shake more ofen? I know my Wine Cap culture likes a good shake to really get good aggressive growth going.
Also, T1 transfer.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
Edited by tree frog (10/30/23 09:24 AM)
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#28524498 - 10/31/23 11:07 AM (2 months, 26 days ago) |
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Quote:
Baba Yaga said: The solution is indeed to shake more often. IME it does get a bit better towards the end. Avoid shaking well progressed jar unless they are absolutely stalling again. In my experience they often look like they don't recover after a late shake although they are totally fine, happens more with vigorous cultures though. Gonna trust your gut when it happens and not your eye.
Thanks.
I took t2 transfers from that plate today. Had it on GWA and 1%. Took two transfers from each onto 1% and inoculated two more jars of millet.
My swab plates germinated one spot so far (out of four plates). Took two transfers from that a few days ago and they seem to be growing out nicely.
Pretty sure at this point, that my original growth was Bisporus and not mold!
Excited to grow, share, and experience what this species has to offer 
And really appreciate your Pans in monotubs thread in particulre. Thanks Baba! It gave me the enouragement to try something new! Only fruited cubes, mexicana, and edibles so far.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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Re: Official Copelandia/Panaeolus Thread [Re: sleepydave]
#28528734 - 11/04/23 08:27 AM (2 months, 23 days ago) |
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I'm trying to get bisporus going and so far it's been really slow on grain (the room I'm running them in is chilly so there's that too).
Anyway, I've been thinking of supplementing graim jars and curious for people's thoughts. I put a few pinches of black kow in a few jars but it's pretty ugly and they're not inoculated yet.
Thoughts on gypsum and seaweed fertilizer? Was thinking I would add both to my soak water. Compost tea, and worm tea from our vermicomposter are other thoughts.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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Re: Official Copelandia/Panaeolus Thread [Re: sleepydave] 1
#28528745 - 11/04/23 08:43 AM (2 months, 22 days ago) |
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Thanks!
I just ordered some seaweed fertilizer and a bag of gypsum.
I'll let you all know how it goes and maybe start a journal to keep track of the experiments.
Also have some reptile heating pads on the way to sort out my temperature issues over winter.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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tree frog
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Re: Official Copelandia/Panaeolus Thread [Re: Bra]
#28528826 - 11/04/23 09:55 AM (2 months, 22 days ago) |
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Quote:
Bra said:
Quote:
tree frog said:
Anyway, I've been thinking of supplementing grain jars and curious for people's thoughts.
My thought is that you could try growing spawn on horse poo and straw/hay mix. 2 parts of poo and 1 part of hay. My favorite pan growing tek for now. The reasons are two - this is very easy and cheap, and this showed to be more resistant to contams than usual grains. Pan mycelium eats this very lovely, look. I love how it looks. It's so inspiring and satisfying how pans myc loves to eat this special.

I gonna try Baba Yaga monotub tek though, because I grow cubes in the monotub and for cubes it's my favorite way so far, so I think I would love pan monotubs too. Just wanted to say that for now in my opinion horsepoo/hay spawn tek is the most resilient in my opinion.
I'll give this a try too. I've been tossing something similar around in my head and I have a bunch of small grow bags I think this would work well for.
Drop a wedge in, knead, let recover, knead again. Maybe cut the bag open lengthwise and lay it on the side once colonized, case and fruit right out of the bag in a fruiting chamber.
That's my thought anyway. I haven't gotten LCs quite figured out or I'd use that instead of a wedge.
edit: In fact, I think I'm going to try making some bags up this afternoon once I get some homework done. How close to standard cube CV field capacity should I do my straw/poo? (I only have black kow poo, so not horse but cow poo).
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
Edited by tree frog (11/04/23 09:57 AM)
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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
#28528887 - 11/04/23 10:41 AM (2 months, 22 days ago) |
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Thanks!
I have a quart of millet that was soaked in coffee for sclerotia not doing anything. Going to mix that in with two quarts of poo, a quart of straw, and a quart of CV.
Hydrate, add gypsum, bag up and PC.
I'll inoculate tomorrow morning. I think it will do better than the straight millet jars I'm trying to run now.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
Edited by tree frog (11/04/23 10:43 AM)
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Re: Official Copelandia/Panaeolus Thread [Re: Bra]
#28528912 - 11/04/23 10:52 AM (2 months, 22 days ago) |
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Field capacity i.e. substrate moisture content.
For cubes this is a few drops of water when you squeeze a handful. No streams just drops.
krikeng said dry side which answered my question.
I'm using straw and coir to help with density issues. I'll keep what you said in mind and maybe add more coir if it's too dense.
The reason I mentioned kneading is because I won't be using grains or lc to inoculate, just a wedge. I don't have pans on grains figured out yet and don't have lcs figured out at all :/
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
Edited by tree frog (11/04/23 10:55 AM)
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Re: Official Copelandia/Panaeolus Thread [Re: Bra]
#28528956 - 11/04/23 11:17 AM (2 months, 22 days ago) |
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Appreciate the word of caution.
It looks like I have enough substrate prepped to do several of these small bags. So I will knead half of them once they recover a bit and leave the other half alone.
I'm at the phenohunting phase of this operation so... not super worried if I lose half the bags as long as I get a few fruits worth cloning.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
Edited by tree frog (11/04/23 11:18 AM)
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#28529331 - 11/04/23 05:07 PM (2 months, 22 days ago) |
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Quote:
Baba Yaga said: How are you going to use the heat mats? Hope you are not planning to put your bag on top of them. Heating spawn unevenly will get you a lot of condensation in the bags.

Right now I just have one reptile pad on the side of a 66 qt tote with spawn jars in it. And a 28 qt tote rests inside of that with my plates. The tote pictured above is a 28 qt tote with three shoeboxes in it. ATL 7 MS, don't care much if it fruits but if it does I'll clone something. My hope is to to try to fruit Pans where the ATL 7 is. Trying to keep my active grows to a minimum as I tend to get carried away otherwise.

The ATL 7 shoeboxes are getting some ambient heat here anyway.

Inside of the incubator.

My thought was to put perlite in the bottom of the 28 qt tote with the shoeboxes in it and the heating pad on the bottom of that just to keep the heat above 72. So, the shoeboxes would rest on the perlite and the heating pad would hopefully difuse the heat more eveny because of the perlite.
To fruit I would dubtub it basically with another 28 qt tote.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28529425 - 11/04/23 06:25 PM (2 months, 22 days ago) |
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My girlfriend doesn't like to run the heat much at night and it's a poorly insulated back bedroom.
Trying to find a compromise to keep the heating bill reasonable.
Last winter we tried putting a space heater in the basement to keep the tent warm enough for a shiitake culture I was running. It didn't work and wasn't cheap.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley]
#28531370 - 11/06/23 05:36 AM (2 months, 21 days ago) |
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Quote:
ruawakeyet said:
Quote:
SupaThaRipper said:
Quote:
tree frog said: My girlfriend doesn't like to run the heat much at night and it's a poorly insulated back bedroom.
Trying to find a compromise to keep the heating bill reasonable.
Last winter we tried putting a space heater in the basement to keep the tent warm enough for a shiitake culture I was running. It didn't work and wasn't cheap. 
What kind of tent we’re you using? Couldn’t have been a good one with the aluminum sidings if it was jacking up your electric bill. I also run my space heater on a heat timer, on the lowest possible setting. Didn’t notice any difference in the electric bill.
Decent grow tents are super cheap on Amazon, if you have access to that. I’ve also got them brand new for even cheaper on FB marketplace. I have 3 6’x6’x7’ lol. Think I payed like $120 for a piece for 2 of them off Amazon and then $50 for the other from FB
If you only heat the tent and not the room, it's hard to deal with the amount of condensation that will occur on the inside of the tent.
I was heating the outside.
It's a mylar tent not a martha.
And the space heater wasn't good to be running in that high of humidity.
Thanks for the heads-up on FB marketplace though. I bought mine on Amazon over COVID. When I need another one I will check FB too.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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Re: Official Copelandia/Panaeolus Thread [Re: SupaThaRipper]
#28534097 - 11/08/23 10:26 AM (2 months, 18 days ago) |
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Quote:
SupaThaRipper said: Yes sir and I do keep my Martha’s inside of Mylar tent as well. Leave the bottom of the Martha’s cracked a touch to and timed exhaust on the Mylar tent directly outside. Cheap tiny heater on low in the Mylar tent. Even with a large Mylar tent, the heater heats it like it’s nothing. I have three separate Mylar tents I do this with and it’s dirt cheap on the electric.
My Mylar tents stay only around 45% on the humidity. So they stay nice and dry even with the Martha’s in them
I would hate to see someone have to shut down during the winter 😔
It's not so bad. I grow cold weather species in the winter and the tent is mostly for edibles. Right now I have some Black Pearl flushing and am looking forward to doing a lot of cooking with them.
Anyway... the incubators are set up and running pretty well. There is some slight condensation in the jars and running plates in it makes visibility terrible but whatever, things are growing and the heat seems to stay around 72 even at night. The ATL 7 is putting up some nice stones too.
Made up four bags of millet, black kow, and coir/verm I had alreay prepped. Dropped one bisporus wedge in each bag and waiting still for visible growth, this was three days ago (I lost track of the wedges fussing with the bags).
Also dropped a bisporus wedge onto an EZLC plate that seems to be doing well so far.
Right now I'm running millet that was soaked in compost tea I made from the black kow. Tomorrow I plan to drop more bisporus wedges in a few quarts of that along with two quarts I have already prepped that have about a tablespoon each of black kow added to them.
So yeah, hoping to get something running soon!
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
Edited by tree frog (11/08/23 10:34 AM)
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] 2
#28538195 - 11/11/23 07:16 AM (2 months, 16 days ago) |
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Just checking in with poo/millet experiments and MS bisporus.
Bisporus inoculated from agar to straight millet a week to ten days ago is super slow. May as well be stalled though it is moving. Shaking didn't seem to help encourage the mycelium to take off either. I'll probably dump these grains and recycle them back into other projects.
Bisporus inoculated to millet soaked in compost tea as well as millet jars with a pinch of compost added directly to it were inoculated morning before last (or maybe night before last). Bisporus is already crawling off the wedge and onto several nearby grains in both sets of jars. Compost tea jars seem a little slower than compost added directly but I think I could dial in the compost tea to work a bit better (higher ratio of tea to water in my grain soak). Also, it's still to early to tell really which are running faster.
But, both are running, which will give me four jars to play with here in a week or two.
Also, made some agar plates with millet water, compost tea, and 0.5% LME. Put two drops of spores on eight plates yesterday. Four of these and four 1% LME. Going to rotate some of my current plates onto these hybrid plates before dropping more wedges on grains. My thought is to train the mycelium on millet before putting it to grains.
I'll check back in with pictures once I have some worth sharing.
As an aside, my incubator maybe holding heat to well. Checked on it this morning, after it had been running all night at 72. Temperature read 84. I assume it had been maintaining at 72 when the heat kicked on this morning in the house and continued to radiate heat from the heating pad and the perlite while the ambient temperature in the room rose. During the day, when the heat in the house is on, it sits at 72 without issues.
Swapping lids to this one to hopefully disipate heat better. Going to stuff with polyfill to keep the casings from drying out (made casing in the dehydrator in a bag and it was stupid easy for small scale casing - made a quart. But with multiple bags and racks it would be easy to scale this up to 8 or more quarts and the temperature of the dehydrator makes it practically set and forget). Still ATL 7 going in it.

The incubator with my cultures/grains was fine this morning, right in the 72-74 range when I checked it. So it's working well and the condensation is minimal.

All that rye in those 3/4 quart jars is ATL 7 (either MS or clones). The quart jars on the left marked PB are the Pans mentioned above.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
Edited by tree frog (11/11/23 07:27 AM)
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Re: Official Copelandia/Panaeolus Thread [Re: SupaThaRipper] 2
#28541149 - 11/13/23 06:27 AM (2 months, 14 days ago) |
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So, this not being the AI picture thread aside...
Thanks to whoever a few pages back suggested sterilizing poo, grain, and whatever else (I went with CV because I had some prepped) and inoculating the bag with an agar wedge.
Spotted some promising growth in one of the four bags when I checked them this morning.
Also got a couple LCs going that look pretty good so far.
The 0.5% LME +50/50 millet water and a few ml compost tea agar doesn't seem to be working out yet. Maybe the myc needs a few days to adapt to it. But the wedges I put on it haven't recovered while the stuff I put on 1% the same day is just smaller than a dime. Could be faint growth too from low nutrient content. I'll have to look a bit closer. I put both an ATL 7 clone and my MS Bisporus culture on it and they both looked the same this morning.
The ATL 7 I'm running is pinning. I'm hoping the fruiting chamber will work well for Pan. Bisporus too.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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Re: Official Copelandia/Panaeolus Thread [Re: MojaveMyc] 1
#28546537 - 11/17/23 02:05 PM (2 months, 9 days ago) |
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Tried the EZLC tek. Inoculated a couple of test jars this morning but looking for feedback as I have had no luck with LCs so far.
Does this look like Bisporus in LC?

Same syringe but settled and with slight condensation (it was in the myco-fridge).

Third shot, after condensation wiped again.
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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Re: Official Copelandia/Panaeolus Thread [Re: RockinRobot]
#28546579 - 11/17/23 02:32 PM (2 months, 9 days ago) |
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Thanks, I'll read through the tek and give it a shot!
-------------------- Listen to the silence behind the engines' noise. Jesus, Sweets, listen. Hear it? It's a love song. For whom? You are loved. ~ David Foster Wallace, Westward the Course of Empire Takes Its Way
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