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rido



Registered: 05/13/19
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Re: Official Copelandia/Panaeolus Thread [Re: CouchShroom]
#26307901 - 11/08/19 06:26 PM (4 years, 2 months ago) |
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Quote:
CouchShroom said:
Quote:
jbgtaa said: No wispy is NOT thick, not ropey
Good to know 
Just saw the examples in Mr. Alien's thread, looks like I've got some more spore germinations & transfers to do
Any chance you have a link to that?
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
#26830818 - 07/18/20 01:39 PM (3 years, 6 months ago) |
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These MEA plates were inoculated from LTC's Pan cyan Rainbow Eucalyptus print almost a week ago. Swabbed a tiny spot on the print, then streaked three plates using the quadrant method without returning to the print. I'm looking to make a few transfers soon before the colonies all run together. Any suggestions, or is it too early to tell?
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#26831034 - 07/18/20 03:44 PM (3 years, 6 months ago) |
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Quote:
Asura said:
Quote:
rido said: These MEA plates were inoculated from LTC's Pan cyan Rainbow Eucalyptus print almost a week ago. Swabbed a tiny spot on the print, then streaked three plates using the quadrant method without returning to the print. I'm looking to make a few transfers soon before the colonies all run together. Any suggestions, or is it too early to tell?

This doesn't look good to me. The Rainbow Eucalyptus print I streaked was extremely bacterial. I swabbed 3 plates and the first 2 were just nothing but bacterial goo. The 3rd plate was better and I was able to make 3 transfers. They are all looking like this pic here, which is exactly the type of myc that I want.

Are you suggesting there's contamination, or that none of the colonies look particularly great phenotype wise? I checked the dates again, those plates were swabbed 4.5 days before I took that picture.
And thanks again, LTC! I've been wanting to try pans for a few years now, looks like it might finally become a reality.
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#26831085 - 07/18/20 04:07 PM (3 years, 6 months ago) |
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Quote:
Adas said: I feel like that's mold. Looks too disorganized for Pan myc.
That could definitely be the case. The germination seemed suspiciously fast. I'll order some sterile swabs and give it another go. Thanks for the input!
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
#26831109 - 07/18/20 04:23 PM (3 years, 6 months ago) |
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Asura said: rido, the prints have contams. I don't know what your process is, I am just saying they are there and I think you have a lot more cleaning up to do.
Luv, this stuff looks really nice. I haven't had growth that looked this good in awhile so I am getting excited about these.
Completely agree. I think the fact that all the growths look the same gave a false sense of security that they were all the right thing, instead of all the same contam. I'd be surprised if they were anything other than contam, really.
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
#26831157 - 07/18/20 04:51 PM (3 years, 6 months ago) |
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Quote:
A.k.a said:
Quote:
rido said:
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Asura said: rido, the prints have contams. I don't know what your process is, I am just saying they are there and I think you have a lot more cleaning up to do.
Luv, this stuff looks really nice. I haven't had growth that looked this good in awhile so I am getting excited about these.
Completely agree. I think the fact that all the growths look the same gave a false sense of security that they were all the right thing, instead of all the same contam. I'd be surprised if they were anything other than contam, really.
He means that getting sterile swabs won’t do anything cuz the contam is in the print. It’s normal that’s what agars for at this point in the process. Its definitely myc in there but it’ll take transfers to get it for sure clean.
I understand there's contam in the print, I just think there were even more contams in the q-tip than the print despite my attempt to sterilize it.
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26838244 - 07/22/20 12:51 PM (3 years, 6 months ago) |
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This is a germ plate nine days after streaking. Does the larger growth look like pan myc? Has anyone else seen that gap in the middle on their germ plates?
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26838290 - 07/22/20 01:07 PM (3 years, 6 months ago) |
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Quote:
A.k.a said: Looks like myc to me but somebody posted similar plates and was told it looked like contam mixed in.
That was me!
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Jakeoncid419 said: Both growths are contam sry bud
No worries, I was pretty sure they were contam, just wanted to be sure.
Thank you both!
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq]
#26840405 - 07/23/20 11:24 AM (3 years, 6 months ago) |
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Tweeq said: Tried to take pics but they only show a flash where the dish is supposed to be
Try taking the picture with a dark background under a lamp with no flash. Getting it just right so you can see the details is tricky but doable.
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
#26846333 - 07/26/20 03:30 PM (3 years, 5 months ago) |
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It's so awesome to read how life changing these mushrooms are for so many of us. It's knocked out my depression and drinking like nothing else can.
I streaked some pan prints five days ago. Both prints from LTC, top row is Rainbow Eucalyptus, bottom is wild coast. I think at least some of this growth is pan myc, but I'm not sure. Some of the growths are fuzzy/tall while others are much flatter.
The second picture is the top middle RE plate, last picture is the bottom right WC plate.
I know it's time to transfer to new plates assuming some of this is pan myc, I'm just not very familiar with what "good" pan myc looks like.
 
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: rido]
#26847067 - 07/27/20 02:00 AM (3 years, 5 months ago) |
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JCM, thank you for those pictures. Would it make any sense to wait for the contams to sporulate to make them easier to ID, or should I try and transfer out from that colony you mentioned before?
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
#26847496 - 07/27/20 09:43 AM (3 years, 5 months ago) |
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Alright round three on germ plates! This time I used a Z streak method instead of overlapping quadrants, and a total of four plates for the single swab instead of three. I also made sure to be much lighter when getting the initial spores from the print. Here's hoping for some usable growth with good separation between the colonies! I also found a nice surprise of a pin on a cube plate this morning, so that's onto its own plate too now.
Thanks again for all the feedback everyone. This hobby has quite a learning curve, but this community makes the process enjoyable no matter what.
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
#26852724 - 07/29/20 10:17 PM (3 years, 5 months ago) |
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Another question from these plates, about 10 days after inoculation: https://www.shroomery.org/forums/showflat.php/Number/26846333#26846333


There's an orange shape on the second picture, it isn't showing up in the thumbnail for me though. Does that look like the type of growth I'm looking for? The growths are so crowded at this point that I don't plan on making any transfer plates from these, just trying to learn. I have a new set of germ plates from a few days ago that are showing growth that has much better separation so far.
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq]
#26873721 - 08/10/20 05:09 PM (3 years, 5 months ago) |
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Quote:
Tweeq said: I seem to be the only one who just drops the spores on to agar from print with a sterile tool.. which, btw, works pretry good
Do i need to get loops? Will that be even more clean?
That works fine, but spreading/streaking helps them not be all lumped together. Loops are better suited to streaking either a liquid culture or spore solution, while a sterile swab works well for streaking from a dry print. Clean is pretty straightforward in this hobby. There are degrees of cleanliness and sterility, sure, but all we typically know or care about is a binary yes/no. Either your process was clean enough, or it wasn't. If you're not having recurrent contam issues I wouldn't worry about it.
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: rido]
#26873727 - 08/10/20 05:17 PM (3 years, 5 months ago) |
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These are both T2 plates from LTC's Rainbow Eucalyptus. Do these look alright to inoculate some LC?
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rido



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Re: Official Copelandia/Panaeolus Thread [Re: Blue Helix]
#26874226 - 08/10/20 10:27 PM (3 years, 5 months ago) |
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Quote:
Blue Helix said: Does anyone know what "rain euc" or "rain eue" might be? I got a print labeled that, but I don't know who sent it. I don't know what it is, but it looks like a pan cyan print of some sort.
Those are from this giveaway: https://www.shroomery.org/forums/showflat.php/Number/26796813
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