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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: grownright]
#22217296 - 09/10/15 03:07 PM (8 years, 4 months ago) |
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Awesome grow, can't wait to get started with pans!! With the spore print you used, did you place the dry spores on your agar in multiple spots on the plate or just in the middle. Also if i were to use an MS syringe, would I just squirt a drop or two in the center and turn the plate from side to side to distribute it? Thanks for the help man
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Fantastic Mr. Fox said:
If your using spore solution in water suspension, I would flame a scalpel or w/e you use for mycologicial/microbiological work then use the tip of the flamed scalpel to place a small "divot" into the center of your dish, then place one drop of solution into the divot. (the divot keeps your spore solution from "running" all over the dish.) Spores need to meet up before they can germinate. They cant do so if your spores are scattered across the dish. Keep it contained..
I'll definitely be trying this out soon.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: MacMerdin]
#22217856 - 09/10/15 05:30 PM (8 years, 4 months ago) |
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Quote:
MacMerdin said: Nice grow.
For extra info on an easy way to do pans see the link in my signature.
Also I just got around to innoculating some cinctulus today. With a variation to my regular pan cake mix. I'll update the process in this thread.
With your "pan-cakes" I assume you used an MS syringe to inoculate through the holes in the dish. What are your contam rates with doing this method, considering that you're using sterilized manure in the mix and an MS syringe that is never 100% sterile..
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: saralove] 1
#22218127 - 09/10/15 06:38 PM (8 years, 4 months ago) |
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AMAZING pics!!
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: saralove]
#22218573 - 09/10/15 08:17 PM (8 years, 4 months ago) |
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Quote:
saralove said: @Psilosoulful @Fantastic Mr. Fox 
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: ICEMANOO9] 1
#22220385 - 09/11/15 06:41 AM (8 years, 4 months ago) |
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: twoseeeye] 2
#22225939 - 09/12/15 07:01 AM (8 years, 4 months ago) |
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.PhilCybin] 1
#22226835 - 09/12/15 12:07 PM (8 years, 4 months ago) |
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Hmm I wonder If I should go with the pan cambos or cyans? Seems like they're both very visual, with cambos having a slightly higher potency
If cultivating from an MS syringe, wouldn't it be difficult to calculate the right dose, being as each mushroom is genetically different and varies in potency. I hear most people say go with 1 gram, but wouldn't that be just an estimation of the strength? I guess you would have to isolate the strain in order to accurately dose, so each dose feels equal in intensity to the last, just like with cubes?
Edited by Psilosoulful (09/12/15 12:08 PM)
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
#22227566 - 09/12/15 03:19 PM (8 years, 4 months ago) |
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If cultivating from an MS syringe, wouldn't it be difficult to calculate the right dose, being as each mushroom is genetically different and varies in potency. I hear most people say go with 1 gram, but wouldn't that be just an estimation of the strength? Do you guys isolate your pan cambo strains to find a monoculture?
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Fantastic Mr. Fox said: Loaded & ready to go!

So you just inoculate those with spores? How are supposed to get a cake out of a jar that big?
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Fantastic Mr. Fox said: LOL Your silly..
I'm using Panaeolus cyanescens LI.
Spores have a hard time germinating on manure, which is why I'm using the liquid culture/innoculant.
&& it wont be a problem.. I'll just spoon it out/or bang against my dirt bike tire, when the time comes. Panaeolus mycelia isnt as dense/brick-like as cubensis.
Lol mb, forgot about using a liquid innoculant. Would it be possible to use an agar wedge to knock up the same jar? I would imagine the colonization rate would be a lot slower tho
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Thanks man, can't wait to start my first pan grow!
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Fantastic Mr. Fox said: I'm rooting for you man! 
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: hummingbird]
#22229237 - 09/12/15 10:02 PM (8 years, 4 months ago) |
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Quote:
hummingbird said: Almost like mescaline IMO…
Haven't tried mescaline yet, fucked up a couple of extractions , I'll get it eventually.
Quote:
hummingbird said: Dried they smell like fish food, kinda weird.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Fantastic Mr. Fox said: There is a god!

Thought this was pretty cool

This guy has a Napoleon complex. Hah,

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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Fantastic Mr. Fox said: Hope everything pans out in your favor. 
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: ICEMANOO9]
#22260958 - 09/19/15 04:26 PM (8 years, 4 months ago) |
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: blackdust]
#22269683 - 09/21/15 02:17 PM (8 years, 4 months ago) |
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Official Copelandia/Panaeolus Thread [Re: nksfo5]
#22291958 - 09/26/15 06:26 AM (8 years, 4 months ago) |
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Got pics of the entire tub setup? I wanna get my pan grow going soon, wanna make sure I don't fuck up the fruiting stage. Not sure if I should do trays or a mini mono? Hmm decisions, decisions...
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