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OfflineDeadPhan
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Anyone ever have a flowhood filter go bad?
    #22216227 - 09/10/15 10:11 AM (8 years, 4 months ago)

So as i try to narrow my source of contams....i honestly think its the environment i work in front of it in. Alot of times also the time of year. If i had good laminar flow shouldnt i be able to work in front of it wherever?  In the 3 years ive had it i find i get alot more contams in the summer weather. Ive had set up in multiple places. Sometimes indoors. Sometimes in old dirty garages. The garages work just fine when in winter or even just not real hot tomes of the year. Leads me to assume theres more in the air in the heat. Obviously but that it still makes its way in. In the winter i have nearly 100 percent success. No matter where. I guess that should lead me to believe my laminar flow might be a tad on the softer side. RR once told me less os better as it doesnt stir up the air and create eddies and draw in contams. I sometimes just worry that my filter might have gone bad. Loke maybe acquired mold. But like i said. Everytime i think that once weather cools it seems to work again. I think my best bet would be to somehow contain the area around the flow. I have some kind of a small visor i attached to the top of the hood. I had in a fairly cool basement with still air and did good a lil while back. Maybe theres drafts in garage but ive had in indoor rooms before where it got contams. I dont know. I think ive seen people male like boxes in front to keep sterile air contained. I camt rememver where or from who. I was thinking just extending 3 walls like on top and two sides. Or suiture a glovebox or sab onto it. Id like not to think my flowhood is shot. Has anyone ever worked in a dirty area with their flowhood and had contam problems. Or should a proper working FH be able to work well in most conditions?  Meh. Just frustrated. Lost alot of spawn this summer to it. I either need to improvise my FH or find a better work area but the latter of the two isnt so easy as i dont have my own place rn. Anyway....


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Big Gulps!  Alright!  Well,  See ya later!
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Invisibleblindingleaf
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Re: Anyone ever have a flowhood filter go bad? [Re: DeadPhan]
    #22216310 - 09/10/15 10:37 AM (8 years, 4 months ago)

i have seen a filter get damaged from a motor that was too powerful.  but it took a year to happen.

im a firm believer in stronger flow than recommended.  100FPM off filter face may be what is written everywhere, but unless one has a legitimate lab, a stronger force, like 150-250FPM, is more desirable.  if anything ends up in the air, u want the force of the hood flow to carry it along the flow, above ur work.

u can do the walls around ur hood, but i would only do that if its 2x3' or bigger.  otherwise, elbows get knocked up and shit.  but I'm also tall and lanky, so i have long arms and boney elbows :crazy:

i would also keep it in a place with minimal humidity.  I'm not sure if mold can grow inside a box plenum if its constantly moving (like ur hood is on for a few hours everyday), but its possible it can grow if the air inside the box has been still for a while (hood gone unused for long time), and its in a warm and humid room.

i know people say the petri dish test is stupid, but i also am a believer in that.  just leave some un inoculated plates open/lid off in front of the running hood for 5 minutes (make sure u run it for an hour or two before beginning).  wrap them up, and see if anything grows.  that way u will know if ur hood is functioning properly.


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Offlinemushpunx
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Re: Anyone ever have a flowhood filter go bad? [Re: blindingleaf]
    #22216399 - 09/10/15 11:05 AM (8 years, 4 months ago)

If you dont already, try spraying the face of the hood down with lysol and let it sit for a minute before you turn it on.

I like everything that BL just said.  Thats what I do too, I do a few dry runs with blank plates and see if anything grows.

I struggle with contam problems too, my house is pretty of full of spores Im sure of it and it cant help. I try to keep the flow hood room spic and span, but can only do so much.

I've wondered the same thing, if you have a good flow hood then you tshould be able to do good work if you stay in the laminar flow regardless of the rest of the room right? Like I think I've seen RR say how he used to run his hood in his carpeted bedroom that his dog sleeps in :lol:

  Do you wipe everything down very well with ISO before it goes in front of the hood? Paying a little more attention to that helped me, as well as sterilizing my tyvek sleeves before I worked so I don't bring mold spores to blow around in front of my hood.

I know how frusterating it is man. Ive been stepping up my sterile tech in all possible areas and still struggle. For example, I thought maybe trich was getting in through my G2G so I did a few tubs with just Agar to Grain, and I still lost 2 out of the 3 tubs. Absolutely mind boggling after being someticulous

I suppose as well, and I might try this for a run too, that you might switch back to still air box for a bit and see if your sucess rate improves :shrug:
Im convinced though, that it must be flaw in my technique SOMEWHERE because my hood seems to work tip top.


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Invisiblestareatclouds
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Re: Anyone ever have a flowhood filter go bad? [Re: mushpunx]
    #22216529 - 09/10/15 11:39 AM (8 years, 4 months ago)



WHEN FLOW HOODS GO BAD


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Invisiblemaddchef
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Re: Anyone ever have a flowhood filter go bad? [Re: mushpunx]
    #22216530 - 09/10/15 11:40 AM (8 years, 4 months ago)

Filters are not perfect and stuff still gets through. Even on my ulpa filter.


Somewhere there is the math but there are x amount of things in the air, like billions. 99.997% still leaves many thousands I think that get through.


This is probably why you see an increase and decrease with seasons. Not much you can do man aside from run a room hepa in your work room 24/7 to cut down ambient load before you even start work. Maybe move to a smaller enclosed area and run your hood a while to scrub the air.

To check for holes you can face a petri towards your filter but you have to be methodical about it, labeling what "quadrant" you tested etc.  If it gets a contam I wouldn't call that a hole. If it is royally fucked up all over I'd call it one.


Hoods are not perfect, not even in the cleanest of labs. Work fast


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In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

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Invisiblemaddchef
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Registered: 09/04/09
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Re: Anyone ever have a flowhood filter go bad? [Re: maddchef]
    #22216543 - 09/10/15 11:43 AM (8 years, 4 months ago)

Also the walls will help only if you're working somewhere drafty. It's likely not stuff being blown into your work area but stuff coming through your filter.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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