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Offlineboats_plus_hoes
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Registered: 06/10/15
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what to do with my pasty plates?
    #22216208 - 09/10/15 10:04 AM (8 years, 4 months ago)

so yesterday i made some pasty plates, all went well except 4 of my 8 plates fell crooked at some point so the agar is crooked, nbd just a smaller angled work space. I also constructed a SAB.

Now im not sure where i should go from here, i have a spore print i produced, which appeared to be purple.

I have a 99% colonized wbs jar that looks good to me, but idk maybe it has wetspot  :shrug:

And lastly i have a nice looking small fruit from an outdoor bed grown from my contamned mono, unfortunatly it was only a small amount of spawn and my big plot flooded. Oh and heres my SAB

keep in mind i only have 4 good plates and 4 slanted ones that will work imo i dont see why not. so should i start from spores, take some grain from that jar and put it on agar (then i think ill g2g for an outdoor grow while I isolate on agar) or cut a piece from the inside of the stem of the fruit?


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OfflineiSmkGrnBud
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Re: what to do with my pasty plates? [Re: boats_plus_hoes]
    #22216468 - 09/10/15 11:22 AM (8 years, 4 months ago)

Spore to agar is very effective. If you want to clone something from that WBS jar I would first fruit it and then use a fresh tissue sample from inside of the fruit to clone, that way you can pick the best looking fruits.

Personally I would start with the spore print to agar. That way you can get a head start on isolating a fresh clean sample. Use as little of the spores as you can when transferring. I find a sterilized cotton swap, or inoculation loop works best.


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Edited by iSmkGrnBud (09/10/15 11:24 AM)


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Offlineboats_plus_hoes
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Re: what to do with my pasty plates? [Re: iSmkGrnBud]
    #22216474 - 09/10/15 11:24 AM (8 years, 4 months ago)

thank you, spores it is, that jar pasty says looks to have bacillus, but i just shook he rup and it smells normal, either way its goin out on my horse shit mound. how can i sterilize q tips. pc?


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OfflineiSmkGrnBud
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Re: what to do with my pasty plates? [Re: boats_plus_hoes]
    #22216486 - 09/10/15 11:27 AM (8 years, 4 months ago)

Quote:

boats_plus_hoes said:
thank you, spores it is, that jar pasty says looks to have bacillus, but i just shook he rup and it smells normal, either way its goin out on my horse shit mound. how can i sterilize q tips. pc?




Yea wrap them up in a couple layers of foil and PC would work. I get single packaged pre-sterilized swabs. They're inexpensive, and a 50 pack last me literally forever.


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Offlineboats_plus_hoes
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Re: what to do with my pasty plates? [Re: iSmkGrnBud]
    #22228581 - 09/12/15 07:12 PM (8 years, 4 months ago)

where'd ya get em?


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OfflineiSmkGrnBud
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Re: what to do with my pasty plates? [Re: boats_plus_hoes]
    #22228613 - 09/12/15 07:19 PM (8 years, 4 months ago)



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“The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi


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OfflineiSmkGrnBud
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Re: what to do with my pasty plates? [Re: iSmkGrnBud]
    #22228618 - 09/12/15 07:19 PM (8 years, 4 months ago)



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“The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi


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