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Offlinefriday126
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Registered: 09/09/15
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Loc: Nashville, TN United Stat...
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Feed Back on Oyster Mushroom First Grow and Contaminate Problems
    #22214601 - 09/09/15 10:07 PM (8 years, 4 months ago)

So I bought Grey Oyster Mushrooms from a local Kroger. Made sure they had plenty of mycelium. Used rubbing alcohol to sanitize a knife and cutting board. Sliced off pieces of mycelium. Used rubbing alcohol to sanitize a bunch of mason jars and coffee filters to substitute for lids (to allow a little air through).
A local coffee shop filled up a 5 gallon bucket with their used coffee grounds. Hoping the hot water used to make coffee properly pasteurized the grounds. The small mushroom pieces with mycelium pushed slightly down into the grounds. After a couple days I started to see the mycelium spread. BUT now there are little blotchy spots that I figure are mold popping up in the jars. Most of what I think is mycelium seems to spread from the original mushroom pieces, while this other (possible mold) seem to pop up where the hell ever. Wonder if I should have done anything to sanitize the pieces of mushroom I cut off to place in the jar or would that just kill the mycelium?
I wonder if I didn't sanitize enough/properly. Or if the coffee grounds sat to long between being collected by the cafe (over about a day) then taken home by me and after work before being put into the mason jars (another day). I must be doing something wrong because these little moldy spots seem to pop up in all 4 jars that started growing mycelium. You can see in one of the pictures below that there seems to be no mycelium and just the invading mold/whatever it is.
I also lightly misted the jars every day or so as they looked very dry. Wonder if something from the air got in them, should I have re-sanitized the jar tops before opening? Any thoughts/feed back welcome. Any thoughts on what the contamination maybe or what to do different next time. Would I be better off with something besides coffee grounds or just get the grounds to a clean growing container much faster and sealed off?
Excuse mediocre pictures. Just the camera I have.
 




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Offlinemidnightmaraude
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Re: Feed Back on Oyster Mushroom First Grow and Contaminate Problems [Re: friday126]
    #22215697 - 09/10/15 07:06 AM (8 years, 4 months ago)

This is all sorts of wrong man!

First off coffee contam rate is high and should be done in a pressure cooker and used as an additive and not a medium in itself. 

Second, you can't just dump chunks of mushroom into the medium.  There are so many contaminates on the mushroom, you need to read on extracting tissue properly from the mushroom and placing it on agar to grow it out.


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Offlinemidnightmaraude
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Re: Feed Back on Oyster Mushroom First Grow and Contaminate Problems [Re: midnightmaraude]
    #22215700 - 09/10/15 07:07 AM (8 years, 4 months ago)

That green shit is trich and the spores are probably blowing all over that room.  Get rid of it asap.


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Offlinenasyn
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Re: Feed Back on Oyster Mushroom First Grow and Contaminate Problems [Re: friday126]
    #22215707 - 09/10/15 07:09 AM (8 years, 4 months ago)

Un-sterilized coffee is not going to work reliably. Cutting pieces of a mushroom up in an unsanitary environment is not going to yield a clean enough piece to inoculate nutritive media.

If you're not planning to do sterilization, and to use mushroom bits, i would suggest cardboard methods.

Otherwise check out the videos and start learning. http://www.mushroomvideos.com/


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OfflineJim Morrison
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Re: Feed Back on Oyster Mushroom First Grow and Contaminate Problems [Re: nasyn]
    #22215756 - 09/10/15 07:30 AM (8 years, 4 months ago)

All of the above.


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Offlinefriday126
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Re: Feed Back on Oyster Mushroom First Grow and Contaminate Problems [Re: Jim Morrison]
    #22216277 - 09/10/15 10:29 AM (8 years, 4 months ago)

Alright thanks guys. Will do some reading (starting with the provided links), sanitize the hell out of everything and start over taking your advice. Maybe starting out with the cardboard method as I learn to keep it simple.


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Offlinemidnightmaraude
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Re: Feed Back on Oyster Mushroom First Grow and Contaminate Problems [Re: friday126]
    #22216294 - 09/10/15 10:33 AM (8 years, 4 months ago)

I would go with the sawdust method.  seems easy. 
learn agar, or buy a syringe from a vendor and knock up some jars, spawn that to more jars and then spawn those to your sawdust.

You'll get so much more from it.

For agar, make your own.  Look up pastyplates make some and experiment with your store bought mushrooms.

To get a culture you rinse the mushroom, pinch the stem of the mushroom breaking it in half.  Take a sterile scapal and scrap some tissue from the middle.  Place on agar.

Of course you'll have to look up still air box too so you can try to keep things sterile.


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