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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 55 minutes
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Ack!
#22207816 - 09/08/15 03:05 PM (8 years, 4 months ago) |
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I've been having of unusual worsening problems over the last few months. I've been using basic techniques for a long time and suddenly I'm seeing side colonies in my agar dishes, dishes stored in certain containers completely being covered with bacteria, unopened control jars getting mold, mushrooms developing spiky bumps near the base, mycelium disappearing from colonized spawn jars, spore prints that should have been practically sterile contaminated with mold spores, etc. Now I've confirmed my suspicions. Thousands of little mites, just barely large enough to even perceive when looking closely and they're all over the area where I keep my spawn jars. Apparently, and I watched as they did this, they can crawl right through cotton and polyfil filters, I also have some jars that use Tyvek filters taped on and they crawl right under the tape. Anyhow, I'm not sure the best way to deal with them. They're so hard to see I don't know how I could possibly be sure I got them all, although they only seem to be present in a small area of one room.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Ack! [Re: Kizzle]
#22207827 - 09/08/15 03:07 PM (8 years, 4 months ago) |
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thats crazy. can u tell if they can get thru parafilm?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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cronicr



Registered: 08/07/11
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RAID AGAR! lol, good luck dude
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Kizzle
Misanthrope


Registered: 08/30/11
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I don't know. I stopped sealing my petris and switched to storing them in filtered cases. Which I guess wasn't a very good idea because they get through the filters
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
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Re: Ack! [Re: Kizzle]
#22207887 - 09/08/15 03:18 PM (8 years, 4 months ago) |
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i saw on a vendor site "sticky mats" for plates, and i always wondered "Why would someone need this?" and i guess ur situation is the answer. i wonder where they came from initially? its kinda scary they are that small. had a gnat problem once, and it was bad cause they would easily go from tub to tub, and i didn't know where they were coming from. i think it was the poo, and i wasn't pasteurizing hot or long enough.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 55 minutes
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Gnats are what I suspected when I first saw the petris, they had little trails on them like something had walked around on the surface. Fortunately the inside of my SAB seems to be clear of them. I have about a dozen plates in it I just poured a few hours before noticing the mites but I got pissed at my roll of cling wrap a while back and threw it away so I don't have anything to seal them with at the moment I suppose I have some ziplock bags but I don't know if that would be effective in keeping them out.
You can see some on this tray. They're the little white specks.
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Edited by Kizzle (09/08/15 04:00 PM)
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Ack! [Re: Kizzle]
#22208156 - 09/08/15 04:25 PM (8 years, 4 months ago) |
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Salt should kill the larvae...I don't know how you would do it, maybe a saltwater solution and let it sit for s day before clean up... any idea what kind of mites they are? I wonder what the life span is on them.
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Q&A US vs. THEM The more I learn, the less I know.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Quote:
blindingleaf said: i saw on a vendor site "sticky mats" for plates, and i always wondered "Why would someone need this?" and i guess ur situation is the answer. i wonder where they came from initially? its kinda scary they are that small. had a gnat problem once, and it was bad cause they would easily go from tub to tub, and i didn't know where they were coming from. i think it was the poo, and i wasn't pasteurizing hot or long enough.
Paul stamets mentions these sticky mats in one of his books. I think it would be a good start. Build a mote of sticky mats around your shit.
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Kizzle
Misanthrope


Registered: 08/30/11
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Last seen: 16 hours, 55 minutes
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I don't know but I think I've seen them once before on a single substrate and simply tossing the substrate was effective. I guess the difference this time is I had a couple of my trays sitting near where I keep my spawn jars and they were just spreading between jars and could have spread from the outside of the jars to some my petris when I doing transfers. It's hard to believe I didn't see them before because now that I'm now that I'm looking for them I'm seeing them on everything.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Ack! [Re: Kizzle]
#22209069 - 09/08/15 07:49 PM (8 years, 4 months ago) |
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Quote:
Kizzle said: It's hard to believe I didn't see them before because now that I'm now that I'm looking for them I'm seeing them on everything.
i hope ur not tripping...
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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ShroominMe
Stranger

Registered: 05/03/15
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I'd pull everything out of my grow room bomb the room with Raid disinfect everything before you put back into the grow room.
No sense dicking around with this imo.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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I agree
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
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Re: Ack! [Re: azur]
#22209277 - 09/08/15 08:28 PM (8 years, 4 months ago) |
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Damn dude that's some poor luck. Hopefully you can get things cleared out and back to normal.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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GoblinKing
Trismegistus

Registered: 08/17/15
Posts: 328
Loc: Right where it belongs.
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Kizzle
Misanthrope


Registered: 08/30/11
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Last seen: 16 hours, 55 minutes
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Once again getting rid of the contaminated substrates (spawn, trays, agar cultures in this case) seemed to be very effective. I can find no mites anywhere, at least none that are large enough to see and are moving.
Here's one more horror pic. This was the worst of the worst buried away in a box of colonizing jars 
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
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Re: Ack! [Re: Kizzle]
#22215882 - 09/10/15 08:13 AM (8 years, 4 months ago) |
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Good to hear! 
That's some down right nasty stuff in that pic. I would have flamed my whole house lol.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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Psilosoulful

Registered: 09/05/14
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Re: Ack! [Re: Kizzle] 1
#22215988 - 09/10/15 08:47 AM (8 years, 4 months ago) |
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Quote:
Kizzle said:

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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
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Man, that sucks, as if we don't have enough to worry about with bacteria and mold. I feel for ya bro, nothing to do but wage all out chemical warfare on those little fuckers
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 55 minutes
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 I think I should to find a way to heat seal these.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 55 minutes
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Re: Ack! [Re: Kizzle]
#22319787 - 10/01/15 03:56 PM (8 years, 3 months ago) |
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This is craziness. These things are still able to get into my unopen jars. I even found some in unopen agar bottle. Hopefully that's the source because I got rid of it. What's really bad is it's not the little white ones I posted a picture of. It's some little reddish ones that can literally only be seen with a microscope. How do you stop something that small?
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Ack! [Re: Kizzle]
#22319793 - 10/01/15 03:57 PM (8 years, 3 months ago) |
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Sticky mats man
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Ack! [Re: azur]
#22319825 - 10/01/15 04:04 PM (8 years, 3 months ago) |
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Just take deep breaths. Let the Mark II Kizzle Filtration system eliminate all the invading organisms.
Just kidding, that's scary. Sticky mats or kill it with fire.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 55 minutes
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I set up some glue boards earlier. They seem to be congregating around a couple of them.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Loc: to the brain
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Re: Ack! [Re: Kizzle]
#22361507 - 10/11/15 01:01 AM (8 years, 3 months ago) |
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Fml...them fuckers are tiny.
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Q&A US vs. THEM The more I learn, the less I know.
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Maybe a light dusting of diatomaceous earth. taking care to not breath any in, of course.
It should dry out their exoskeleton, assuming they have one.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
Posts: 891
Loc: Your subconscious
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Re: Ack! [Re: Grey]
#22362015 - 10/11/15 07:47 AM (8 years, 3 months ago) |
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I helped my friend and his uncle clean an old nasty ass dope heads apartment once, covered in fleas and mites and all sort of creepy crawlys. He told me to buy some of what Grey said, and me and my buddy laid waste to the apartment with it. The very next day like 95% of them were dead, just piles of bugs everywhere. I felt like Hitler.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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Kizzle
Misanthrope


Registered: 08/30/11
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I haven't seen any live mites today so hopefully that's because they're dead. I think I figured out why they were rampant on top of my SAB and yet none inside it. There's a layer of tape surrounding the entire opening with part of the adhesive still exposed and they apparently can't crawl over the sticky part of it without getting stuck. So I'm going to try setting up a box with tape on the edges like that to keep my petris and jars in.
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invitro

Registered: 05/03/13
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Re: Ack! [Re: Kizzle]
#22366576 - 10/12/15 02:42 AM (8 years, 3 months ago) |
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I had those mites, or ones very similar to them when I first started cultivating... It took me a while to figure out what the problem was. Flipping the metal lids so the rubber was up was providing a way in for them. Rubber down and sfds put a stop to their activities.
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Kizzle
Misanthrope


Registered: 08/30/11
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Things are starting to looking up. For the first time in over a month I have culture that remained clean long enough for some spores to germinate. I'm not sure if that's because they're gone or because I used flat half-pint jars instead of petris but I've made agar plates so I'll be finding that out soon. I was kind of worried for a bit that they may have gotten into and contaminated all my spore prints.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
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Re: Ack! [Re: Kizzle]
#22396336 - 10/18/15 05:11 AM (8 years, 3 months ago) |
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where do u think they started from? did u move recently?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Quote:
Kizzle said: Here's one more horror pic. This was the worst of the worst buried away in a box of colonizing jars 

I bet this is where they came from. Or at least where they were able to first get a big foothold established and then spread from. Bug bomb and bleach bomb the area.
Quote:
invitro said: Flipping the metal lids so the rubber was up was providing a way in for them. Rubber down and sfds put a stop to their activities.
This stuff right here is why I always advocate using the lids correctly with the rubber side down, as intended. And also the use of SFDs over other filters. There are lots of people, even TCs that do use lids the wrong way and recommend it to others. If mites can get in, you have unfiltered GE.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Quote:
blindingleaf said: where do u think they started from? did u move recently?
They originated from a tray of his I do believe.
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Q&A US vs. THEM The more I learn, the less I know.
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Kizzle
Misanthrope


Registered: 08/30/11
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They were probably carried in by gnats. I don't have a gnat problem or anything because I have fly paper up all over the place but they get in pretty easily and occasionally I catch one buzzing around my FC. I'n pretty sure they got to the cultures when I had a tray stored near them. That was around the same time I started to notice the bacteria trails appearing in my cultures. The FC was absolutely swarming with them. The only other place I found any was in some empty jars that had been spawned but not rinsed out afterward.
I use most of my lid right-side up but the sealant from them has been removed and until recently the lids weren't loose, but weren't tightened all the way either. In at least one jar it appeared they weaseled past the imperfections in the tape that was holding a Tyvek filter on but I seems they were mainly getting in under the lids and through some of the lids that used cotton filters. They also got into a bottle of agar, by going under the cap I suppose.
I'm not a mite expert but it appeared there were at least 3 different species.
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: Ack! [Re: Kizzle]
#22405803 - 10/19/15 09:59 PM (8 years, 3 months ago) |
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Spoke too soon I guess, again.
 More mite trails.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Ack! [Re: Kizzle]
#22405824 - 10/19/15 10:02 PM (8 years, 3 months ago) |
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Ugh
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Re: Ack! [Re: azur]
#22405835 - 10/19/15 10:04 PM (8 years, 3 months ago) |
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Fuck bro...
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Q&A US vs. THEM The more I learn, the less I know.
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Kizzle
Misanthrope


Registered: 08/30/11
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I managed to get some clean cultures and have done a bunch of transfer with no contamination so I'm thinking the problem is solved. I inoculated some spawn jars for the first time in a couple months and they're cruising along and I haven't seen a live mite for quite a while. I've been taking a lot of precautions so I'm not sure which helped. I changed all the filters to non-organic tape, set up the glue and tape traps, and the temperature has really cooled down so that might have played a role too.
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