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Offlineamyloid
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Registered: 03/08/03
Posts: 980
Last seen: 2 years, 10 months
anyone care to explain this?
    #2220719 - 01/05/04 04:07 PM (12 years, 10 months ago)

ok so i innoculated 4 mycobags with several differnt strains. multispore innoculation. nothing colonized but one with PES H., i broke it up and let it regrow, then i cut the bags and added pc'd popcorn. but now(1 week later) there isnt any growth. not even on the peices of grain that were once innoculated!? this sound similar to anything youve all encountered? the bags were kept @ 82f +-2 since innoculation.


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Invisibleplura16
ROM3/10 SOLID

Registered: 09/25/03
Posts: 574
Loc: U.S.A.
Re: anyone care to explain this? [Re: amyloid]
    #2220769 - 01/05/04 04:28 PM (12 years, 10 months ago)

how long did you wait before "nothing colonized"?

adding new substrate to bags that havent colonized yet is generally a bad idea since you have such a good chance to introduce contams. could have just inocced some new popcorn with the pesh and waited a bit longer for the other bags to grow.

colonization time can be affected by your spores, syringe tek, and sub prep.

how good can a mycobag be if its opened and closed and the contents altered?

no growth on the original sub after 1 week -- and after adding new sub--- what was the time frame you waited before this? doesnt sound entirely abnormal, given we dont know time frames.


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Offlineamyloid
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Registered: 03/08/03
Posts: 980
Last seen: 2 years, 10 months
Re: anyone care to explain this? [Re: plura16]
    #2221169 - 01/05/04 07:22 PM (12 years, 10 months ago)

innoculated 12.10 broke up > a week later, then i added the popcorn about a week ago when it looked close to completely colonized.. i couldnt innoculate the corn cause i had no more spore juice, i had used it all trying to get some growth in the a seperate jar of corn.


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"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
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Invisibleplura16
ROM3/10 SOLID

Registered: 09/25/03
Posts: 574
Loc: U.S.A.
Re: anyone care to explain this? [Re: amyloid]
    #2222334 - 01/06/04 06:05 AM (12 years, 10 months ago)

if you broke up the jar less than a week after multispore innoculation -- it may have been too soon, and prolonged colonization.

grain to grain transfer might help you out if you dont have a ny more spore solution.

it may just be that - for whatever factors -- these bags are coming along slower than what you are used to?


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- USA -


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Amazon Shop for: ½ Pint Jars, Mycobags

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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