|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
CubensisFrenzy


Registered: 07/15/15
Posts: 27
Last seen: 8 years, 3 months
|
|
Quote:
TheShroomanizer said: Sorry quick question about sterilizing used syringes. Is the standard tek, Take whole syringe including needle/needle cap/resevoir/ wrap in alluminum foil and PC at 15 psi for 30 mins?
That's what RR's video suggests. I'd stick with that. half hour isn't too long. As long as there's water in the syringe you won't see any melting.
--------------------
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
 don't put water in your syringe...it will just come back empty anyway lol you can put them in dry they won't melt and 30 minutes is not too long but also longer then needed
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
CubensisFrenzy


Registered: 07/15/15
Posts: 27
Last seen: 8 years, 3 months
|
Re: Lighting Frequencies [Re: cronicr]
#22207864 - 09/08/15 03:14 PM (8 years, 4 months ago) |
|
|
Quote:
cronicr said: I'm not saying your wrong to do so , people also grew in fishtanks with drip shields do as you will i will always suggest poly your fruits are screaming for fae...all i'm saying as for your link, like i said peopel did lots of fucked up shit 10 years ago lol, we know better now...at least most of us do
2008 isn't ancient and Polyfill was used way before that.
--------------------
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
he was an old school dude! trust me i know faht well and like this hobby he has grown as well doesn't change the fact that your fruits are kindly asking for more fae
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
CubensisFrenzy


Registered: 07/15/15
Posts: 27
Last seen: 8 years, 3 months
|
Re: Lighting Frequencies [Re: cronicr]
#22207907 - 09/08/15 03:21 PM (8 years, 4 months ago) |
|
|
Quote:
cronicr said:
 don't put water in your syringe...it will just come back empty anyway lol you can put them in dry they won't melt and 30 minutes is not too long but also longer then needed
I realizing disagreeing with people like you is pointless and a waste of energy but, just for the sake of simplicity why wouldn't tell newbs to stick to the video? Just to feel more important? An empty syringe will melt at a certain point. Flame sterilizing an empty syringe needle begins to melt it in seconds. With liquid inside you could accidentally leave the PC for hours and be fine.
--------------------
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
no the water in the syringe won't stay there...that's what i'm saying, there is no flames inside of a pc and you do not need any liquid in the syringe what soever
Quote:
RogerRabbit said: Boiling water IS enough to sterilize a syringe. Plastic does not harbor bacterial endospores, and fungi like trichoderma and cobweb are killed by temperatures far below boiling.
I fill a pot with water and drop the syringe in. After the water has boiled for ten minutes, pull the syringe out and suck in the boiling water, swish it around and squirt it out. Do this a few times. The syringe will be sterile enough for mycology, I assure you. I've done it this way for years. RR
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
|
Re: Lighting Frequencies [Re: cronicr]
#22207960 - 09/08/15 03:30 PM (8 years, 4 months ago) |
|
|
This thread is a joke.
Cronicr is only trying to help you Phillyman he has 40000 posts Jesus fuck.
Take his advice say thank you, you don't have to agree or do it. He is aware you can do things multiple ways but he is giving you the most proven methods to current
|
CubensisFrenzy


Registered: 07/15/15
Posts: 27
Last seen: 8 years, 3 months
|
Re: Lighting Frequencies [Re: invitro]
#22207963 - 09/08/15 03:31 PM (8 years, 4 months ago) |
|
|
Quote:
invitro said:
Quote:
CubensisFrenzy said:
  
Sometimes I'll rotate the tubs mid-fruiting. Notice the curvature of the stems...
  
See the third pic, how there are dimples in some of the caps? Is that bacteria starting or? What causes that?
Quote:
invitro said:
Quote:
CubensisFrenzy said:
  
Sometimes I'll rotate the tubs mid-fruiting. Notice the curvature of the stems...
  
See the third pic, how there are dimples in some of the caps? Is that bacteria starting or? What causes that?
I'm pretty sure you're talking about the white spots on the caps?? That's mycelium...

" Mycelium on the caps is not an indication of too much humidity. It's a combination of two types of mycelium on the fruiting body. It's more genetic in nature, occuring in some substraiins more than others. RR"
--------------------
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
i'm sure he is talking about the actual dimple looking things which is from standing water
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
|
if you dont wanna listen to cron at least listen to your mushrooms..
|
CubensisFrenzy


Registered: 07/15/15
Posts: 27
Last seen: 8 years, 3 months
|
Re: Lighting Frequencies [Re: cronicr]
#22207984 - 09/08/15 03:35 PM (8 years, 4 months ago) |
|
|
Quote:
cronicr said: no the water in the syringe won't stay there...that's what i'm saying, there is no flames inside of a pc and you do not need any liquid in the syringe what soever
Quote:
RogerRabbit said: Boiling water IS enough to sterilize a syringe. Plastic does not harbor bacterial endospores, and fungi like trichoderma and cobweb are killed by temperatures far below boiling.
I fill a pot with water and drop the syringe in. After the water has boiled for ten minutes, pull the syringe out and suck in the boiling water, swish it around and squirt it out. Do this a few times. The syringe will be sterile enough for mycology, I assure you. I've done it this way for years. RR
My 2 points were simplicity for 1st timers and cheap vendor syringes melting. Maybe you're misunderstanding me but, your points are off target.
--------------------
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
i think you're missing the point....have you ever pc'd a syringe? here i'll make it simple, wrap the empty syringes in tinfoil and pc for 30 minutes or boil them
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
CubensisFrenzy


Registered: 07/15/15
Posts: 27
Last seen: 8 years, 3 months
|
Re: Lighting Frequencies [Re: cronicr]
#22208010 - 09/08/15 03:41 PM (8 years, 4 months ago) |
|
|
Quote:
cronicr said: i think you're missing the point....have you ever pc'd a syringe? here i'll make it simple, wrap the empty syringes in tinfoil and pc for 30 minutes or boil them
That's better. If I was doing it for the 1st time that'd be much easier to understand.
--------------------
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
i get that but all i was telling you was not to put water in the syringe before you pc it
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
CubensisFrenzy


Registered: 07/15/15
Posts: 27
Last seen: 8 years, 3 months
|
|
I was posting info on 6500k lights for the op and the community. Most people understand things better with visuals so I post pictures.
You catty bitches need to relax and get off your man's nuts.
--------------------
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
Quote:
CubensisFrenzy said: Most people understand things better with visuals so I post pictures.
agreed that's why i posted the .. it didnt' seem to help you though
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
blackdust


Registered: 02/28/09
Posts: 8,327
|
Re: Lighting Frequencies [Re: cronicr]
#22208045 - 09/08/15 03:52 PM (8 years, 4 months ago) |
|
|
Quote:
cronicr said: muffin just boil the syringes for about 10 minutes then suck up some bopiling water and squirt it out and repeat a couple times then leave it in there one last time till it cools
and this is why everybody pushes agar. That is not how to make a clean syringe.
|
TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
|
|
CubensisFrenzy, you have one of the best growers and teachers on this site giving you some help, take it. Unless your syringe is totally closed system, pressure cooking the syringe with water inside will not work, the water will boil out. There are no "cheap" vendor syringes which will melt.
cron, your patience does this to me:

Quote:
You catty bitches need to relax and get off your man's nuts.
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Lighting Frequencies [Re: cronicr]
#22208054 - 09/08/15 03:56 PM (8 years, 4 months ago) |
|
|
Quote:
blackdust said:
Quote:
cronicr said: muffin just boil the syringes for about 10 minutes then suck up some bopiling water and squirt it out and repeat a couple times then leave it in there one last time till it cools
and this is why everybody pushes agar. That is not how to make a clean syringe. 


Quote:
cronicr said:
Quote:
RogerRabbit said: Boiling water IS enough to sterilize a syringe. Plastic does not harbor bacterial endospores, and fungi like trichoderma and cobweb are killed by temperatures far below boiling.
I fill a pot with water and drop the syringe in. After the water has boiled for ten minutes, pull the syringe out and suck in the boiling water, swish it around and squirt it out. Do this a few times. The syringe will be sterile enough for mycology, I assure you. I've done it this way for years. RR
hi bd...miss me?
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
|
Re: Lighting Frequencies [Re: blackdust]
#22208058 - 09/08/15 03:57 PM (8 years, 4 months ago) |
|
|
Quote:
blackdust said:
Quote:
cronicr said: muffin just boil the syringes for about 10 minutes then suck up some bopiling water and squirt it out and repeat a couple times then leave it in there one last time till it cools
and this is why everybody pushes agar. That is not how to make a clean syringe. 
Everybody pushes agar because it allows you to secure a clean culture. I don't like tossing 100s of quart jars, and I don't like sitting around wondering if the material I'm inoculating with may or may not be clean. Maybe I'm weird.
That WILL make a clean spore syringe, period. I've done it this way many, many times. You want to know how I know this, besides that fact that I've seen repeated success with this technique? Take up some boiled water, aspirate it, take it up, aspirate, take it up, leave it. Now drop some of this water on a sterile slide and view it. Tell me what you see.
Edited by TheEaglesGift (09/08/15 04:03 PM)
|
|