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OfflineKenneth
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Registered: 06/07/15
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I need to clarify some stuff
    #22205187 - 09/07/15 11:40 PM (8 years, 4 months ago)

I am starting on the journey to growing shrooms soon but i need to clarify how i will do it. I will feel my jars with rye grain and sterilize them in a pressure cooker. Then i will drop my spores in the jars and cover the lids with foil and will put them in a box to let them colonize in a dark cool place. Once i see they are colonized i will do a monotub tek such as this one. I will dump the jars in the montub and break it up to spread it around the whole tub. Then i will add a casing layer of coco coir. I will have lamp shining on it and maybe a dehumidifier if im lucky. After all that i will wait until they fruit where i will pick them and attempt to make spore prints. Will this work? please give any tips/advice you have.


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InvisibleMad Season
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Re: I need to clarify some stuff [Re: Kenneth]
    #22205252 - 09/07/15 11:59 PM (8 years, 4 months ago)

You cover the lids with foil for the pc so water doesn't get into the jars. You remove the foil when you're ready to inoculate. No foil for colonization!! Shake the grains at 30% colonization. What kinda filters/lids are you planning on using? Are you going to inoculate in a still air box?

For both cloning and for starting up with a clean culture.. I'd highly recommend starting your spores on agar. Look into agar some.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (09/08/15 12:10 AM)


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OfflineKenneth
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Re: I need to clarify some stuff [Re: Mad Season]
    #22205300 - 09/08/15 12:15 AM (8 years, 4 months ago)

I Will be using a still air box but i will be using a syringe to inoculate so i thought the foil would cover the holes i make with that to protect from contaminants. As for the agar i have no idea how to do that i will have to look into it more, does it take up alot of time/money?


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InvisibleMad Season
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Re: I need to clarify some stuff [Re: Kenneth]
    #22205310 - 09/08/15 12:18 AM (8 years, 4 months ago)

Under $20 if you can find it locally. It's usually found in Asian markets or the Asian aisle of big stores.

If you are inoculating in a still air box, just pop the lid of the jar, and squirt 3 drops a jar with your flamed needle. You gotta get good at taking the lid off, especially with agar work. Check out the improve your sterile techniques link in my signature man :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (09/08/15 12:19 AM)


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OfflineKenneth
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Re: I need to clarify some stuff [Re: Mad Season]
    #22205351 - 09/08/15 12:31 AM (8 years, 4 months ago)

ok great post man it is really helpful could you explain the concept of agar to me?


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InvisibleMad Season
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Re: I need to clarify some stuff [Re: Kenneth]
    #22205362 - 09/08/15 12:36 AM (8 years, 4 months ago)

http://www.shroomery.org/forums/showflat.php/Number/18430998

Isolating a clean culture free of all contams. Since spores can contain contams (you'd be surprised how many syringes are contaminated, but can easily be saved with agar). That and cloning.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (09/08/15 12:37 AM)


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OfflineKenneth
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Posts: 244
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Re: I need to clarify some stuff [Re: Mad Season]
    #22205404 - 09/08/15 12:49 AM (8 years, 4 months ago)

Ok I am making a trade on here for a syringe do you think i could skip the agar part this time? As i am currently really limited on funds


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InvisibleMad Season
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Re: I need to clarify some stuff [Re: Kenneth]
    #22205420 - 09/08/15 12:55 AM (8 years, 4 months ago)

Give it a shot man :smile:. You don't need agar. I cultivated successfully without it for a long time. It'd just give you a better success rate. I still highly recommend 3 drops a jar though. It'd really stretch the syringe out, and limit the # of contams from the syringe going into the jars (if there are any).


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleBig Bear
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Re: I need to clarify some stuff [Re: Kenneth]
    #22205513 - 09/08/15 01:30 AM (8 years, 4 months ago)

my first few monotubs I just shot spore syringes directly in to grain, with success.  Here is the tek I used.  Hope it helps.
http://www.shroomery.org/forums/showflat.php/Number/19792837


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OfflineAchillita
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Re: I need to clarify some stuff [Re: Big Bear]
    #22205588 - 09/08/15 02:13 AM (8 years, 4 months ago)

Just a spore syringe will work fine for first time cultivating. Agar is pretty great though. I'd suggest looking into Pasty plates if you wanna do agar, as you can reuse the containers.

I grew pretty successfully without agar for a whiel aswell. THe only reason I got into agar is because I couldn't make a spore syringe correct or it wouldn't be sterile... So I raged and gave up. Agar is pretty easy, and since moving to it, I have yet to have a single contam.


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OfflineKenneth
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Re: I need to clarify some stuff [Re: Big Bear]
    #22207804 - 09/08/15 03:02 PM (8 years, 4 months ago)

That tek has alot of supplies you need how much did it cost you total?


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InvisibleBig Bear
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Re: I need to clarify some stuff [Re: Kenneth]
    #22208604 - 09/08/15 06:15 PM (8 years, 4 months ago)

around 150.


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Invisibledreadhed
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Re: I need to clarify some stuff [Re: Achillita]
    #22209469 - 09/08/15 09:14 PM (8 years, 4 months ago)

Quote:

Achillita said:
Just a spore syringe will work fine for first time cultivating. Agar is pretty great though. I'd suggest looking into Pasty plates if you wanna do agar, as you can reuse the containers.

I grew pretty successfully without agar for a whiel aswell. THe only reason I got into agar is because I couldn't make a spore syringe correct or it wouldn't be sterile... So I raged and gave up. Agar is pretty easy, and since moving to it, I have yet to have a single contam.



Quote:

Achillita said:
Just a spore syringe will work fine for first time cultivating. Agar is pretty great though. I'd suggest looking into Pasty plates if you wanna do agar, as you can reuse the containers.

I grew pretty successfully without agar for a whiel aswell. THe only reason I got into agar is because I couldn't make a spore syringe correct or it wouldn't be sterile... So I raged and gave up. Agar is pretty easy, and since moving to it, I have yet to have a single contam.



Quote:

Achillita said:
I'd suggest looking into Pasty plates if you wanna do agar, as you can reuse the containers.

I grew pretty successfully without agar for a whiel aswell. THe only reason I got into agar is because I couldn't make a spore syringe correct or it wouldn't be sterile... So I raged and gave up. Agar is pretty easy, and since moving to it, I have yet to have a single contam.



Quote:

Achillita said:
Just a spore syringe will work fine for first time cultivating. Agar is pretty great though. I'd suggest looking into Pasty plates if you wanna do agar, as you can reuse the containers.

I grew pretty successfully without agar for a whiel aswell. THe only reason I got into agar is because I couldn't make a spore syringe correct or it wouldn't be sterile... So I raged and gave up. Agar is pretty easy, and since moving to it, I have yet to have a single contam.




I agree a syringe will work for grains & that agar is better, but you can reuse petri dishes. I've got some that are years old and work just fine. Some times I use tupperware when the dishes are all used up but I like the dishes better.


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InvisibleMad Season
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Re: I need to clarify some stuff [Re: dreadhed]
    #22209517 - 09/08/15 09:22 PM (8 years, 4 months ago)

Reuse plastic petri dishes? :lol: okay.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineiSmkGrnBud
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Re: I need to clarify some stuff [Re: Mad Season]
    #22209644 - 09/08/15 09:51 PM (8 years, 4 months ago)

Maybe he meant those expensive ass glass petri's? :shrug:


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“The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi


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Invisibledreadhed
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Re: I need to clarify some stuff [Re: iSmkGrnBud]
    #22221302 - 09/11/15 10:47 AM (8 years, 4 months ago)

Quote:

iSmkGrnBud said:
Maybe he meant those expensive ass glass petri's? :shrug:



I did. I would have thought Mad season would have know that's what I was talking about. But yes I reuse glass not plastic dishes.


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OfflineiSmkGrnBud
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Re: I need to clarify some stuff [Re: dreadhed]
    #22222094 - 09/11/15 01:47 PM (8 years, 4 months ago)

Well its just that no one here can justify spending that much money on a single petri dish. :shrug:


--------------------
:bigweed:  iSmkGrnBud's Teks  :bigweed:
:mushroomgrow: iSmkGrnBud's Wild Mushroom Finds :mushroomgrow:
:amanita2: The Noob Forum's List of Teks :amanita2:

“The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi


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Invisibledreadhed
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Re: I need to clarify some stuff [Re: iSmkGrnBud]
    #22222612 - 09/11/15 03:32 PM (8 years, 4 months ago)

I can. I've had them since 1997 maybe 98. They aren't the cheapest way to go but if you use them for years it's cheaper than plastic & less trash.


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OfflineAchillita
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Re: I need to clarify some stuff [Re: dreadhed]
    #22222727 - 09/11/15 03:56 PM (8 years, 4 months ago)

I just use pasty plates, re usable, no trash, and easy to work with. I've also found they hold moisture for months at a time without drying out.

I was gonna buy glass, but god damn they are expensive.


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OfflineiSmkGrnBud
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Re: I need to clarify some stuff [Re: Achillita]
    #22222952 - 09/11/15 04:50 PM (8 years, 4 months ago)

:whathesaid:


--------------------
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:mushroomgrow: iSmkGrnBud's Wild Mushroom Finds :mushroomgrow:
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“The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi


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