|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22214768 - 09/09/15 10:48 PM (8 years, 4 months ago) |
|
|
|
kmetric



Registered: 08/23/14
Posts: 140
Last seen: 7 years, 10 months
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Inocuole]
#22214780 - 09/09/15 10:50 PM (8 years, 4 months ago) |
|
|
OP, I've used spore syringe to agar many times successfully. Don't squirt too much, that's all.
Keep reading and reading, and good luck.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: kmetric]
#22214786 - 09/09/15 10:51 PM (8 years, 4 months ago) |
|
|
Helps if you flame the needle and use the hot ass needle to make a little divot where you're going to squirt. Just be sure to clean it off and flame the needle again afterwards so there's no agar growing shit on it.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22214789 - 09/09/15 10:52 PM (8 years, 4 months ago) |
|
|
Quote:
6Silent9Knight6 said:
Quote:
van der griegen said:
Quote:
6Silent9Knight6 said: If you have a syringe I would not go to agar. Syringes usually contaminate on agar for some reason but you could get lucky and have some clean myc to transfer.

Spore solution to agar is great. Those same contaminants were going to germinate in your seed. 
That's what you think....
OP... Do us a favor and shoot up the syringe on agar and tell us how it goes. And then... with that same syringe... shoot up a cake. Tell us how it goes.
These grows were all done with spore syringe to agar. For obvious reasons. . .


|
Leviticus969



Registered: 07/01/12
Posts: 939
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Inocuole]
#22214799 - 09/09/15 10:54 PM (8 years, 4 months ago) |
|
|
I never said they can't be done... I'm just saying they contaminate easier versus prints.... That's all I said.
Pretty much I've never had a print contaminate on me from throwing on agar but had a higher ratio of contaminates from syringes to agar.
This is my experience and opinion. No need for the flames.
Edited by Leviticus969 (09/09/15 10:56 PM)
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22214803 - 09/09/15 10:55 PM (8 years, 4 months ago) |
|
|
Quote:
6Silent9Knight6 said: I'm just saying they contaminate easier versus prints.... That's all I said.
And that's EXACTLY why you want that to happen on agar and not your substrate. There were no flames. Only facts.
|
Leviticus969



Registered: 07/01/12
Posts: 939
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Inocuole]
#22214811 - 09/09/15 10:58 PM (8 years, 4 months ago) |
|
|
Well... From MY experience... I've shot the same syringe on agar and onto a few pf jars. The jars all made it but my agar plates contammed horribly.
So... That's my fact. Don't ask me how that happened or why it did. It just did.
Edited by Leviticus969 (09/09/15 10:58 PM)
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22214815 - 09/09/15 10:59 PM (8 years, 4 months ago) |
|
|
What flames? You said to knock up a brf jar and a plate and see how it went. I posted a pic of grows that I did with ms solution to agar. Its not like I facepalmed you or called you something insulting. Jesus I can't post shit without people getting butthurt it seems.
To be entirely honest I have had very little issue with syringes to agar providing the syringe was reasonably clean. Some are a lost cause but they would have done not better on grains or cakes either. Cleaning up stuff is the point of agar.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22214816 - 09/09/15 10:59 PM (8 years, 4 months ago) |
|
|
Agar is supposed to show contaminates. That just means all those cakes probably underperformed because they were contaminated. If you had used the agar to get a clean culture and THEN put that to the cakes, it would probably have yielded significantly better results.
It's not like you're trying to grow off the agar, agar is supposed to contaminate if there are contaminants. That's the point.
|
Leviticus969



Registered: 07/01/12
Posts: 939
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Pastywhyte]
#22214826 - 09/09/15 11:02 PM (8 years, 4 months ago) |
|
|
sorry Pastye... lol... You're alright man... That wasn't directed towards you. We good bro hahah
Oh and btw... Nice grows
Edited by Leviticus969 (09/09/15 11:02 PM)
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22214828 - 09/09/15 11:03 PM (8 years, 4 months ago) |
|
|
|
Kalistis


Registered: 09/06/15
Posts: 2,265
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22214928 - 09/09/15 11:32 PM (8 years, 4 months ago) |
|
|
Quote:
6Silent9Knight6 said:
Quote:
van der griegen said:
Quote:
6Silent9Knight6 said: If you have a syringe I would not go to agar. Syringes usually contaminate on agar for some reason but you could get lucky and have some clean myc to transfer.

Spore solution to agar is great. Those same contaminants were going to germinate in your seed. 
That's what you think....
OP... Do us a favor and shoot up the syringe on agar and tell us how it goes. And then... with that same syringe... shoot up a cake. Tell us how it goes.
I'm not sure where you jumped in on this thread but I have over 10 years in a clinical setting and 5 years in a laboratory setting mixing, pouring, and plating selective growth and nutrient rich media for diagnostic purposes. Isolations are just another day at the office... What I don't have is experience plating fungal spores with the intention of using the agar for cultivation. As a biologist, the idea of trusting that someone else has used sterile techniques to produce my inoculate without testing it first, terrifies me. But I also don't have enough experience cultivating to know if I'm overreacting by going with my gut.
--------------------
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Kalistis]
#22214935 - 09/09/15 11:34 PM (8 years, 4 months ago) |
|
|
Sounds like you know just enough to go ahead and go with your gut. Do everything yourself and do it well.
|
Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Kalistis]
#22217966 - 09/10/15 06:02 PM (8 years, 4 months ago) |
|
|
Quote:
Caelistis said:As a biologist, the idea of trusting that someone else has used sterile techniques to produce my inoculate without testing it first, terrifies me. But I also don't have enough experience cultivating to know if I'm overreacting by going with my gut.
Semi-sterile. Many of the people who sell spore syringes source their spores very responsibly, but that still just means they source them from people who grow mushroom reliably in their house, clear out subs as soon as they show trich instead of waiting, use sterile foil for prints and they printed it in a SAB or glove box. It notably does NOT mean the mushrooms were not fruited in open air in a house--or even a monotub--that has never had a trich infection.
I will say this: every syringe has a contaminant in it. It may be something small and unnoteworthy: a weakly-growing Bacillus, a Pennicilium spore. It may be something awful, like a Pleurotus or Trichoderma spore. The better the vendor, the more care they take finding a spore source. But if you have the tools and the skills, they're screaming to be used anyway. Might as well use best practices.
|
Leviticus969



Registered: 07/01/12
Posts: 939
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Psilicon]
#22218654 - 09/10/15 08:42 PM (8 years, 4 months ago) |
|
|
Quote:
but that still just means they source them from people who grow mushroom reliably in their house, clear out subs as soon as they show trich instead of waiting, use sterile foil for prints and they printed it in a SAB or glove box. It notably does NOT mean the mushrooms were not fruited in open air in a house--or even a monotub--that has never had a trich infection.
Sorry don't mean to get a lil off track here but....
What if someone leaves their tub full of triched out substrate for awhile in the room... What does that mean? All grows forward would be contaminated? Or maybe it means shrooms grown in that room won't be potent anymore? Or perhaps the rest of your grows will catch trich faster? (trich on 2nd flush)
I'm really curious to know... Lmk if I need to start my own thread.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22218667 - 09/10/15 08:44 PM (8 years, 4 months ago) |
|
|
Quote:
6Silent9Knight6 said: All grows forward would be contaminated? Or maybe it means shrooms grown in that room won't be potent anymore? Or perhaps the rest of your grows will catch trich faster? (trich on 2nd flush)
The third one but POSSIBLY the first one if your technique really sucks. Contaminants don't affect the potency of mushrooms generally, except MAYBE for a slight correlation some have noticed between bacterial subs and potency, but there have been no tests run on that.
Also, one thing you forgot to hypothesize- You could die. If it were, you know, some toxic mold and it had filled the room with spores.
|
Leviticus969



Registered: 07/01/12
Posts: 939
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Inocuole]
#22218717 - 09/10/15 08:52 PM (8 years, 4 months ago) |
|
|
Thanks. Interesting response... I would love to hear more about this... Especially from someone who's had trich in their room and still does grows with good potency in said room.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22218740 - 09/10/15 08:56 PM (8 years, 4 months ago) |
|
|
13shrooms says he can't grow in his house anymore. Could maybe pop in the AMU Q&A and ask him, since he's mostly in there. That's not the demographic you're looking for but he obviously has experienced contamination hell.
Most of us have had trich at one time and still produce good potency grows though. I don't know how many have let tubs get entirely forest green and sit for days and still grow though. I'm imagining a good few. But we all move and clean house and such now and then.
|
Leviticus969



Registered: 07/01/12
Posts: 939
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Inocuole]
#22218748 - 09/10/15 08:58 PM (8 years, 4 months ago) |
|
|
I will pop in there.... Thanks, once again. Still looking forward to responses here also.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22218760 - 09/10/15 09:01 PM (8 years, 4 months ago) |
|
|
You can have successful grows after trich. But the higher the sporeload from any species the more likely things go awry. Filters can fail more often due to high spore loads on them, still air work will be less forgiving of sloppy technique, spawn runs may have slightly more difficulty completing clean. Obviously some good cleaning is a start but its still good practice to keep things as generally clean as you can.
Its about percentages. A filter with a sporeload of 1, 000, 000 spores is more likely to fail than a filter with 500, 000.
|
|