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Kalistis

Registered: 09/06/15
Posts: 2,265
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Munchauzen]
#22210371 - 09/09/15 12:23 AM (8 years, 4 months ago) |
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Poop. I did not know this. FFS... I have 50 pounds of shit seed? I will verify the label before I commit to feeding the neighborhood birds for the next 10 years with my 50 pound bag. The feed store had 50 pound bags of millet as well. Would that be better?
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Leviticus969



Registered: 07/01/12
Posts: 939
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Kalistis]
#22210437 - 09/09/15 12:46 AM (8 years, 4 months ago) |
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If you have a syringe I would not go to agar. Syringes usually contaminate on agar for some reason but you could get lucky and have some clean myc to transfer.
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Kalistis

Registered: 09/06/15
Posts: 2,265
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22210445 - 09/09/15 12:48 AM (8 years, 4 months ago) |
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Why couldn't I just isolate out the contaminants?
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Leviticus969



Registered: 07/01/12
Posts: 939
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Kalistis]
#22210452 - 09/09/15 12:51 AM (8 years, 4 months ago) |
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You don't want to transfer contaminants.... You want to transfer clean and healthy mycelium to another plate. Don't even touch those contaminants unless you plan on throwing that scalpel away right after.
But like I said... You could get some clean myc but sometimes there won't be any clean myc to transfer because of the sterility of the syringe.
I'd prepare some cakes or whatever you're trying to do and inoc to that. But you could knock your agar plates up at the same time and see for yourself also as a learning experience
Edited by Leviticus969 (09/09/15 12:54 AM)
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Kalistis

Registered: 09/06/15
Posts: 2,265
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Kalistis]
#22210461 - 09/09/15 12:55 AM (8 years, 4 months ago) |
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That's exactly what I mean't transfer out the healthy spores to a new plate and leave contaminants, removing them from my sample. I have experience doing just that, but I could also do g2g transfer, but I am pretty worried about the contaminants in my syringes. Honesty, had I thought this one through more I would have started with a spore print, but that I have no experience with and thought the syringes would be a better place to start.
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Kalistis

Registered: 09/06/15
Posts: 2,265
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Kalistis]
#22210467 - 09/09/15 12:56 AM (8 years, 4 months ago) |
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That's a good idea... Perhaps I will start one master grain jar for g2g in the event my agar goes to shit.
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Leviticus969



Registered: 07/01/12
Posts: 939
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Kalistis]
#22210472 - 09/09/15 12:59 AM (8 years, 4 months ago) |
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Print to agar is better than syringe to agar, tenfold, imho.
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Kalistis

Registered: 09/06/15
Posts: 2,265
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22210482 - 09/09/15 01:03 AM (8 years, 4 months ago) |
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Well... I have these 3 syringes to work with first. Should all go well, I will make my own prints for future runs.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Kalistis]
#22210521 - 09/09/15 01:27 AM (8 years, 4 months ago) |
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nothin' wrong with a spore syringe to agar...
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Ajahn Don
Stranger


Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 9 months, 1 day
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Munchauzen]
#22211163 - 09/09/15 08:25 AM (8 years, 4 months ago) |
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The only hard part is getting one drop out of a new syringe.
-------------------- "He's not altogether dense, but he's not altogether there."
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Ajahn Don]
#22211389 - 09/09/15 09:42 AM (8 years, 4 months ago) |
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squirt a drop on your loop and then swipe the loop on the agar! I dont have the attention span to read that one huge ass post Op made..sorry
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,674
Last seen: 3 hours, 25 minutes
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: spacechildo]
#22211459 - 09/09/15 10:01 AM (8 years, 4 months ago) |
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Op, Make some pasty plates then try all the suggestions and pick the one that is most comfortable to you
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: spirit_shadow]
#22212883 - 09/09/15 03:50 PM (8 years, 4 months ago) |
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I was planning on trying some different medium nutrients in the process as well. I usually do loop to agar transfers because I will definitely squirt more than 1 drop otherwise.
Is straight millet better than seed mixes?
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THP
Stranger

Registered: 05/21/15
Posts: 123
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Kalistis]
#22213613 - 09/09/15 06:10 PM (8 years, 4 months ago) |
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I have read straight millet is not ideal and can be hard to work with, either do straight rye, milo (sorghum), or wild bird seed mix with milo, millet as the two main ingredients, wheat is okay to have in it you just don't want corn (Makes it very sticky and fucks with moisture content I think) or fruits. I am only starting out too though so take my advice with a grain of salt
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: THP]
#22214510 - 09/09/15 09:41 PM (8 years, 4 months ago) |
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The 50 pound bag does have cracked corn in it. I read about some techniques using gypsum (which I purchased), but I have decided to scratch the current WBS and pick up another bag. I suppose I could take it back to the feed store ask if I could exchange it for a bag without cracked corn.
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Ajahn Don
Stranger


Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 9 months, 1 day
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Kalistis]
#22214636 - 09/09/15 10:17 PM (8 years, 4 months ago) |
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Try oats at the feed store. I got mine at a Cal Store.
-------------------- "He's not altogether dense, but he's not altogether there."
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Leviticus969]
#22214663 - 09/09/15 10:24 PM (8 years, 4 months ago) |
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Quote:
6Silent9Knight6 said: If you have a syringe I would not go to agar. Syringes usually contaminate on agar for some reason but you could get lucky and have some clean myc to transfer.

Spore solution to agar is great. Those same contaminants were going to germinate in your seed.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Psilicon]
#22214683 - 09/09/15 10:27 PM (8 years, 4 months ago) |
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I think that's the guy who used to burn his "bad trip" cubes in a bonfire. Don't worry about him van.
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Inocuole]
#22214719 - 09/09/15 10:34 PM (8 years, 4 months ago) |
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Hey, you know me, Inoc. I worry.
OP, continue as planned. And make sure you drink plenty of fluids.
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Leviticus969



Registered: 07/01/12
Posts: 939
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Re: First Cultivation- To Agar or Not to Agar? That is the question. [Re: Psilicon]
#22214747 - 09/09/15 10:43 PM (8 years, 4 months ago) |
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Quote:
van der griegen said:
Quote:
6Silent9Knight6 said: If you have a syringe I would not go to agar. Syringes usually contaminate on agar for some reason but you could get lucky and have some clean myc to transfer.

Spore solution to agar is great. Those same contaminants were going to germinate in your seed. 
That's what you think....
OP... Do us a favor and shoot up the syringe on agar and tell us how it goes. And then... with that same syringe... shoot up a cake. Tell us how it goes.
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