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tedoro
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Making a larger amount of LI
#22201079 - 09/07/15 08:58 AM (8 years, 4 months ago) |
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Hi Ya'll
I've been noc'ing up large, pre-sealed myco bags with LI I've made from squirting and sucking up water in a petri dish. I can see what appears to be tons of mycelia floating in the syringe, but my bags are 4 quarts and its taking them 6 days to show growth. (about 8 bags per petri, but not all the petri dish is sucked up) (two 60mm syringes)
I'd like to make a larger quantity of dense LI. Ideas? I've been enjoying not getting into blenders, but I will if need be.
T
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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MushLover2552
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Re: Making a larger amount of LI [Re: tedoro]
#22201101 - 09/07/15 09:03 AM (8 years, 4 months ago) |
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Based on my first GLC/LI experience yesterday, I'd say go for the blender. About 23 hours after nocc'ing up 12 jars, 7 of them are recovering strong  I found the oster 14 speed as frequently used in teks for $20 New on ebay
Edited by MushLover2552 (09/07/15 09:03 AM)
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iSmkGrnBud
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Re: Making a larger amount of LI [Re: tedoro]
#22201122 - 09/07/15 09:09 AM (8 years, 4 months ago) |
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Quote:
tedoro said: Hi Ya'll
I've been noc'ing up large, pre-sealed myco bags with LI I've made from squirting and sucking up water in a petri dish. I can see what appears to be tons of mycelia floating in the syringe, but my bags are 4 quarts and its taking them 6 days to show growth. (about 8 bags per petri, but not all the petri dish is sucked up) (two 60mm syringes)
I'd like to make a larger quantity of dense LI. Ideas? I've been enjoying not getting into blenders, but I will if need be.
T
Maybe use a pint sized jar instead of a petri for some agar, or maybe something like pasty's? Something with more volume so you can add more water into it. If you have a good growth of mycelium, it should have no problem making that much LI. Could make a few syringes at a time.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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tedoro
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Re: Making a larger amount of LI [Re: iSmkGrnBud]
#22201127 - 09/07/15 09:12 AM (8 years, 4 months ago) |
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Hot tips. Has anyone made a slurry substance that can be injected? If I'm going to be messing around with a blender, I might be open to slurry... I'm just so set on my pre-sealed mycobags. They are delightful to use. But I gotta inject them.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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MushLover2552
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Re: Making a larger amount of LI [Re: tedoro]
#22201137 - 09/07/15 09:15 AM (8 years, 4 months ago) |
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You could always suck some of the grain-mycelium-water out of the jar and then re-inject, not too familiar with the terms but isn't that basically what a GLC is?
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tedoro
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I need to read more about mycelia grain water, sounds like it could be less clean than a petri dish. But yeah, that sounds great and fast.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Xochi Phil
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Re: Making a larger amount of LI [Re: tedoro]
#22202746 - 09/07/15 03:41 PM (8 years, 4 months ago) |
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http://www.shroomery.org/forums/showflat.php/Number/16071143#16071143
This thread and and another by Agar are all you need on the subject.
These presealed bags...You dont ever make your own spawn or anything? Sounds costly to use premade anything. Sterilizing a grain jar or two is simple enough.Wish you luck eh
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tedoro
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Re: Making a larger amount of LI [Re: Xochi Phil]
#22204364 - 09/07/15 08:37 PM (8 years, 4 months ago) |
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The bags cost .60 cents. Its a whole monotub worth of grain. I grown enough mushrooms in each mono that its an inconsequential cost. Pros... a ton of grains in one PC loading. Zero contams from it being sealed before leaving the PC. I love myco bags. Jars are so not efficient in the pc, I'm not a fan.
T
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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maddchef
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Re: Making a larger amount of LI [Re: tedoro]
#22204822 - 09/07/15 10:06 PM (8 years, 4 months ago) |
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What bags are you presealing, link?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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tedoro
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Re: Making a larger amount of LI [Re: maddchef]
#22206102 - 09/08/15 08:16 AM (8 years, 4 months ago) |
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-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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tedoro
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Re: Making a larger amount of LI [Re: tedoro]
#22206199 - 09/08/15 08:45 AM (8 years, 4 months ago) |
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I just read "grain liquid culture tek" It sounds perfect for me. I've been soaking my grains before I mix them in substrate and have always been amazed by the water I pour off. Huge chunks of mycelia!
I am somewhat sad about my current petri squirting/sucking LI. It looks as though 1 month in the refrigerator did not do it well. Its been 7 days, no sign of growth. I think it might work, but This is exactly why I need to change things up, I gotta get that mycelia winning in there sooner than that.
t
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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iSmkGrnBud
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Re: Making a larger amount of LI [Re: tedoro]
#22207977 - 09/08/15 03:34 PM (8 years, 4 months ago) |
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Glc is pretty good when wanting to knock up a lot of donor jars, but there are some precautions you definitely want to be aware of before working with it. Most importantly you have to know without a doubt that your grain spawn is 100% clean amd contam free. You wouldn't want to contaminate all your jars. How many jara are you working with. I find g2g the preferred method when wanting to make a lot of jars from just a few masters.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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Aero
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Re: Making a larger amount of LI [Re: tedoro]
#22208328 - 09/08/15 05:17 PM (8 years, 4 months ago) |
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Quote:
tedoro said: Hi Ya'll
I've been noc'ing up large, pre-sealed myco bags with LI I've made from squirting and sucking up water in a petri dish. I can see what appears to be tons of mycelia floating in the syringe, but my bags are 4 quarts and its taking them 6 days to show growth. (about 8 bags per petri, but not all the petri dish is sucked up) (two 60mm syringes)
I'd like to make a larger quantity of dense LI. Ideas? I've been enjoying not getting into blenders, but I will if need be.
T
make a big jar of no pour of agar im talking about 1 quart size, add your culture and wait till fully colonized once its done, cut the agar and blend it then add the mix to 2 liters of sterile water and keep the whole thing incubated for a week or two ull have 2liters of inoculant very fast
using a magnetic stir plate speeds up the process
the mycelium will grow rapidly as the nutritious agar is readily available in the solution
simple
-------------------- SPREAD THE SPORES
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tedoro
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Re: Making a larger amount of LI [Re: Aero]
#22209175 - 09/08/15 08:10 PM (8 years, 4 months ago) |
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Aero,
Is agar less prone to contamination than standard LC? I'm interested in LI, and it seems that having less nutrition helps. I've been worried about a procedure with tons of agar in it for that reason.
Maybe I'm wrong.
T
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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cronicr



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Re: Making a larger amount of LI [Re: tedoro]
#22209206 - 09/08/15 08:15 PM (8 years, 4 months ago) |
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agar and lc have the same amount of nutes in each recipe, lc has more available nutes though as agar only colonizes the surface, as for contams agar is easier to spot them but no less prone to them
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iSmkGrnBud
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Re: Making a larger amount of LI [Re: tedoro]
#22209214 - 09/08/15 08:16 PM (8 years, 4 months ago) |
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Quote:
tedoro said: Aero,
Is agar less prone to contamination than standard LC? I'm interested in LI, and it seems that having less nutrition helps. I've been worried about a procedure with tons of agar in it for that reason.
Maybe I'm wrong.
T
It's not really that it's less prone to contamination, but when working with agar, it gives you the option of separating clean growth from a contamination, and transferring it to clean agar, so you don't dont waste a specimen. LC is hard to tell if there is a contamination, and if there is, it most likely has effected all of your LC.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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Xochi Phil
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Re: Making a larger amount of LI [Re: tedoro]
#22209496 - 09/08/15 09:19 PM (8 years, 4 months ago) |
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Quote:
tedoro said: The bags cost .60 cents. Its a whole monotub worth of grain. I grown enough mushrooms in each mono that its an inconsequential cost. Pros... a ton of grains in one PC loading. Zero contams from it being sealed before leaving the PC. I love myco bags. Jars are so not efficient in the pc, I'm not a fan.
T
I wasn't in my right mind after work and had the thought that you were buying bags already stuffed with pasteurized grain,like one of those goofy kits. I have yet to try bags, but 60cents and being able to fit more in my 30 quart pc sounds like a time saver. I'll give em a shot.
Edited by Xochi Phil (09/08/15 09:21 PM)
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tedoro
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Re: Making a larger amount of LI [Re: Xochi Phil]
#22210367 - 09/09/15 12:22 AM (8 years, 4 months ago) |
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Yeah.... these bags are amazing. I'm really impressed with them. I working my entire process around them. So I am interested in LI because of how liquid in a syringe works so well with presterilized mycobags. Ans I am trying to figure out how to talk about non-nutritious LI culture because it seems to be the jam with low contamination. Agar is blended or mixed into mycelia and it seems to me to be similar to Liquid Culture. But people talk of agar as only petri dishes. I think Agar pureed into water is a liquid culture just as malt or honey is. Correct me if I'm wrong.
T
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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midnightmaraude
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Re: Making a larger amount of LI [Re: tedoro]
#22211109 - 09/09/15 08:08 AM (8 years, 4 months ago) |
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Yesterday I squirted a small vile of blue oyster myc into a pint of PC'd water and Karo (after cooling of course). The myc had been in the fridge since December. I hope it recovers and grows. How long should one expect to see growth in the jar?
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maddchef
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Re: Making a larger amount of LI [Re: cronicr]
#22211348 - 09/09/15 09:29 AM (8 years, 4 months ago) |
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Quote:
cronicr said: agar and lc have the same amount of nutes in each recipe, lc has more available nutes though as agar only colonizes the surface, as for contams agar is easier to spot them but no less prone to them
Lol wut? So you're saying my lc recipe should be 10g lme per 500ml water? That mfer would be ridiculously cloudy no?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Aero
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Re: Making a larger amount of LI [Re: tedoro]
#22211557 - 09/09/15 10:30 AM (8 years, 4 months ago) |
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Quote:
tedoro said: Yeah.... these bags are amazing. I'm really impressed with them. I working my entire process around them. So I am interested in LI because of how liquid in a syringe works so well with presterilized mycobags. Ans I am trying to figure out how to talk about non-nutritious LI culture because it seems to be the jam with low contamination. Agar is blended or mixed into mycelia and it seems to me to be similar to Liquid Culture. But people talk of agar as only petri dishes. I think Agar pureed into water is a liquid culture just as malt or honey is. Correct me if I'm wrong.
T
correct but with agar, u can be 100% sure that what u put into the liquid is clean, LC not so much
-------------------- SPREAD THE SPORES
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tedoro
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Re: Making a larger amount of LI [Re: Aero]
#22220636 - 09/11/15 08:03 AM (8 years, 4 months ago) |
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To clarify, When I say Agar in this thread, I am meaning agar that is blended into water with mycelia. I would have a tendency to cal that LC not LI. Not sure how others feel about that. And I'm interested in LI.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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bodhisatta 
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Re: Making a larger amount of LI [Re: tedoro]
#22220723 - 09/11/15 08:25 AM (8 years, 4 months ago) |
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Agar to water = LI Agar to nutrient water = LC
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tedoro
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Re: Making a larger amount of LI [Re: bodhisatta]
#22220973 - 09/11/15 09:23 AM (8 years, 4 months ago) |
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But people who blend a whole agar plate into water are seeing grown after the fact. Isn't that a grey area?
T
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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bodhisatta 
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Re: Making a larger amount of LI [Re: tedoro]
#22220992 - 09/11/15 09:26 AM (8 years, 4 months ago) |
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Maybe a little bit. But I wouldn't call it a LC.
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