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Cloudbase Jim
Stranger



Registered: 08/07/15
Posts: 46
Last seen: 8 years, 1 month
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Another mold or mycelium post
#22200718 - 09/07/15 06:39 AM (8 years, 4 months ago) |
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Hello! I'm just wanting to know if I have finaly had some success. I know that this was a bit early to go opening petri dishes but I wanted a better look at what was inside.
So waht do you think? Is it myc or mold?
Thanks


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Cloudbase Jim
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Registered: 08/07/15
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In case it's relevant, this is 7 days after inoculation on MEA
Edited by Cloudbase Jim (09/07/15 08:08 AM)
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Cloudbase Jim said: Hello! I'm just wanting to know if I have finaly had some success. I know that this was a bit early to go opening petri dishes but I wanted a better look at what was inside.
Why didn't you open it in your SAB, you just exposed your culture to millions of airborne contams….
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UncleFester
Lord Moldy-Butt



Registered: 09/23/14
Posts: 460
Loc: Where The Sun Don't Shine...
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I get similar looking spots on some agar plates of mine, I can't exactly tell you that they're not contamed yet because I haven't seen it developing. I'm going to assume it's pieces of mycelium that touched the agar below or when you were placing the inoculant
--------------------
Looking for Penis Envy & Psilocybe Cubensis Cuba spores.
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Cloudbase Jim
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Re: Another mold or mycelium post [Re: UncleFester]
#22200969 - 09/07/15 08:31 AM (8 years, 4 months ago) |
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I have like 10 of these all looking the same. I just decided to use this one for observations.
So what's your verdict Psilosoulful?
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Cloudbase Jim said: I have like 10 of these all looking the same. I just decided to use this one for observations.
So what's your verdict Psilosoulful?
Separate this dish from the rest. The contams that landed on the dish after you opened it will grow out. There is still a slim chance that mycelium will grow, but you'll have to carefully take a small healthy sample and transfer to another sterile dish. Keep an eye on the other ones. It's too early to tell if it is mold or not.
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Cloudbase Jim
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Re: Another mold or mycelium post [Re: Psilosoulful]
#22201159 - 09/07/15 09:22 AM (8 years, 4 months ago) |
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Thanks Psilosoulful, you have helped me a lot so far. I was going to place the dish back with the others but now I have it re-wraped in parafilm in a separate drawer. I'm not fussed about this dish getting contaminated because I have backups but I'd have been gutted if it contaminated my other dishes.
I'll post pics up again once I see some more development.
Cheers
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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No problem man
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Another mold or mycelium post [Re: Psilosoulful]
#22201194 - 09/07/15 09:39 AM (8 years, 4 months ago) |
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when u take plate pics, hold it up to the light. u want the light from ceiling fan/etc to be shining on TOP of the plate. if u light it from behind, the texture of the mycelium can be hard to make out

when u don't hold it up to the light, the flash will make a glare, or if at the wrong angle (like in ur picture) the glare will prevent good visibility

this is ideal

worst comes to worst, put it on a white piece of plastic (like a tub lid or white cardboard) and take the shot from straight above, with no flash
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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if thats from spores and you got same amount of growth on other plates its time to do some transfers (yesterday).
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Cloudbase Jim
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Re: Another mold or mycelium post [Re: spacechildo]
#22201343 - 09/07/15 10:20 AM (8 years, 4 months ago) |
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OK thanks Blindingleaf, I'm afraid that the auto focus on my phone won't let me get any better shots than this for now. I'll try and get better with a decent camera ASAP
spacechildo, this is the very first sign of growth. There doesn't appear to be any rizomorphic growth at the moment, just fuzz with a cottony lump at the center. Where would you transfer?
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NewToCultivation
New Cultivator


Registered: 08/31/15
Posts: 185
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Any Contam on this Jar? when i look close at the myc in person the dark spots look like the myc hasn't gotten thick enuff to cover the colour of the substrate.. but i could be wrong..
-------------------- everything I do or say is for informational purposes only I do not condone the cultivation or sales of any illegal substances. Oh yeah, and i fucked bodhisatta's mother.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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It'll cover the substrate, just give it some time. And take that foil off, it traps moisture on the jar lid, which creates a perfect climate for contams to grow.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Quote:
Cloudbase Jim said: spacechildo, this is the very first sign of growth. There doesn't appear to be any rizomorphic growth at the moment, just fuzz with a cottony lump at the center. Where would you transfer?
transfer from the leading edge. dont bother looking for rhizomorphic mycelium, its just a reaction to lowered nutrition in the agar recipe. what you really want is to just get clean myc. by transferring clean myc to a new plate you hopefully leave any potential contams left on the old plate.

why is there foil on the jar? and how big is that jar? looks good so far IMO. also, make your own thread next time
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NewToCultivation
New Cultivator



Registered: 08/31/15
Posts: 185
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Re: Another mold or mycelium post [Re: spacechildo]
#22203011 - 09/07/15 04:35 PM (8 years, 4 months ago) |
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Im sorry, the site won't let me create a new thread.. i am very new to this and this is my very first grow, Just signed up like a couple days ago... thanks tho... and if i take the foil off won't contams get in the jar?
-------------------- everything I do or say is for informational purposes only I do not condone the cultivation or sales of any illegal substances. Oh yeah, and i fucked bodhisatta's mother.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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yes it will, just follow the link! the beginners forum you cant start threads in but Mush Cult (here) you can.
you have a dry verm barrier dont you? that's your filter from contams. dont disturb this dry verm barrier, it needs to sit tight to do its job so you shouldnt really pick up pf-jars.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
NewToCultivation said: and if i take the foil off won't contams get in the jar?
If you have a dry verm layer(which you should) contams will get trapped by that layer and will be unable to get to the brf/verm mix.
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NewToCultivation
New Cultivator



Registered: 08/31/15
Posts: 185
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Re: Another mold or mycelium post [Re: spacechildo]
#22203038 - 09/07/15 04:42 PM (8 years, 4 months ago) |
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yeah i have a dry verm barrier, and the jar is 250ml .... and yeah this site is very confusing to me atm but ill get the hang of it soon
-------------------- everything I do or say is for informational purposes only I do not condone the cultivation or sales of any illegal substances. Oh yeah, and i fucked bodhisatta's mother.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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It's confusing to everyone when they first sign up. You'll def get used to it and hopefully become a valuable contributor to the forums.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Quote:
NewToCultivation said: yeah i have a dry verm barrier, and the jar is 250ml .... and yeah this site is very confusing to me atm but ill get the hang of it soon
OK, remove the foil and leave the cake alone for a week at least before looking at it again. top of the mush cult forum right above all the threads it says Post. Press it to make your own thread or just press this direct link
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