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Offlinebennylava
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Registered: 05/29/15
Posts: 587
Last seen: 6 years, 9 months
Nothing after 3 days?
    #22200562 - 09/07/15 03:52 AM (8 years, 4 months ago)

Did the violet grass seed tek, but with BRF since I'm but a lowly noob. Maybe move on to the grass seed once I've got a bit more experience.

But its been 3 days, and I don't see any little white patch anywhere. Using clear ziplock brand containers and the standard BRF mix. Humidity seems to be doing well. I had them in the top of my closet, because it was a bit warmer up there. I kept checking the temp with my IR temp gun though, and it was only about 78 at most. So I moved them above the fridge, into the standard cabinet that everyone has above their refrigerator.

The bottom of the ziplock container now reads 86 degrees, and the sides read 83. Big improvement there. I'd say on average its something like 84 degrees inside the cabinet. Been checking it fairly regularly.

I know 3 days isn't long, but I just wanted to report what I've been doing and see if anyone thinks I've been doing anything wrong. Some people have a dime sized white spot at about 4 days, and for me it looks like its going to be a good deal longer than that. I'm not impatient, I just want to make sure all is well. Thanks for reading


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OfflinePabloSumgie
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Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
Re: Nothing after 3 days? [Re: bennylava]
    #22200574 - 09/07/15 04:03 AM (8 years, 4 months ago)

Multy spore syringe I assume. It could take up too a couple weeks. Most guys now a day I will incubate at room temp with indirect light. 86 is hot. The higher temps are more favourable for bacteria and other nasties. All my stuff incubates at 68 degrees.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!


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Offlinebennylava
Bad example


Registered: 05/29/15
Posts: 587
Last seen: 6 years, 9 months
Re: Nothing after 3 days? [Re: PabloSumgie]
    #22200581 - 09/07/15 04:09 AM (8 years, 4 months ago)

Quote:

PabloSumgie said:
Multy spore syringe I assume. It could take up too a couple weeks. Most guys now a day I will incubate at room temp with indirect light. 86 is hot. The higher temps are more favourable for bacteria and other nasties. All my stuff incubates at 68 degrees.





Yeah it was a syringe. I was going off of that old (but not real old) PF tek video that everyone has seen. IIRC they said 78 to 90 degrees F. I heard that lower temps, such as your 68, make it take a lot longer to colonize. So I was just trying to expedite the process.


Edited by bennylava (09/07/15 04:10 AM)


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Offlinexpsprogamer
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Registered: 07/17/15
Posts: 98
Loc: NorthEast U.S.
Last seen: 3 years, 3 months
Re: Nothing after 3 days? [Re: bennylava]
    #22200591 - 09/07/15 04:18 AM (8 years, 4 months ago)

I incubat at 85f-86f and u haven't had n e contamination issues yet. I know im still new to this but I have preped about 30 jars and have 4 big lasagna pans going right now at about 76f in the fruiting chamber. IMO just watch them and see what works for you. there are people that will tell u that 76 is better for incubating but as long as your sterile then it should start to colonize. if it isn't then go back and practice your sterile tek and make sure ur cake misture is correct. I use rye grain and don't know much about PK teks.im sure there are way more intelligent people in this tek.


--------------------


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InvisibleInocuole
Scalpel of Evil's Bane
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Re: Nothing after 3 days? [Re: xpsprogamer]
    #22200600 - 09/07/15 04:30 AM (8 years, 4 months ago)

In other words, fuck up until it really starts to affect you, THEN start taking advice from the right people.  Granted this is on deaf ears since OP has me ignored.  But hey that's the kind of mentality we're promoting here right?  Don't like an answer?  Pretend it never existed.

For everyone else, the best "incubation" temps overall, not for speed, but for overall integrity, are 68-73F.  Having the mycelium grow 15% faster is not worth everything else growing 5x as fast.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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OfflinePabloSumgie
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Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
Re: Nothing after 3 days? [Re: Inocuole]
    #22200604 - 09/07/15 04:34 AM (8 years, 4 months ago)

Ya it comes down to personal preference and what works for you. The higher temps do speed it up significantly but its a higher chance of contams but if your sterilization is 100% hypothetically there should be no problems. So its up too you.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!


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InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Nothing after 3 days? [Re: PabloSumgie]
    #22200606 - 09/07/15 04:38 AM (8 years, 4 months ago)

Contamination is almost always present in some form.  A number of elements factored against each other can quickly make or break a grow.  There are people who will tell you which risks to take, and which to play safe.  Squandering that is... well..  like I said, OP will never get any of this information.  As far as he knows, 84 degrees is GREAT.  So yeah, it's pretty terrible practice.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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OfflinePabloSumgie
Stranger

Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
Re: Nothing after 3 days? [Re: Inocuole]
    #22200613 - 09/07/15 04:44 AM (8 years, 4 months ago)

Yup I like what your saying. I shoulda stated that sterilizing to 100% and keeping it there is next to impossible what your really doing is giving your spores/myc the best chance of starting first finishing first beating out the rest. The rest will always be there your just giving the myc the fighting chance. Colonizing at high temps is working against your master plan even if it doesn't seem that way.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!


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OfflineAjahn Don
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Registered: 07/05/15
Posts: 482
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Re: Nothing after 3 days? [Re: PabloSumgie]
    #22201110 - 09/07/15 09:06 AM (8 years, 4 months ago)

Nobody has said anything about the "3 days" complaint, either. One of the hardest things about this hobby is patience. I was reading an article about the SF 49ers doing things in blocks of 30 minutes because young people today have an attention span of 28 minutes. We want it, and we want it right now. If we have practiced our mindfulness more anywhere than here, we'd be surprised. We both have a strong meditation practice, but this was a real challenge.

I've given up on a lot of people who will not listen. Maybe I'm more aware of it, but it seems to be an increasing problem everywhere. I don't know why it seems so much more noticeable here.


--------------------
"He's not altogether dense, but he's not altogether there."


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Offlinebennylava
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Registered: 05/29/15
Posts: 587
Last seen: 6 years, 9 months
Re: Nothing after 3 days? [Re: Ajahn Don]
    #22201139 - 09/07/15 09:16 AM (8 years, 4 months ago)

Quote:

Ajahn Don said:
Nobody has said anything about the "3 days" complaint, either. One of the hardest things about this hobby is patience. I was reading an article about the SF 49ers doing things in blocks of 30 minutes because young people today have an attention span of 28 minutes. We want it, and we want it right now. If we have practiced our mindfulness more anywhere than here, we'd be surprised. We both have a strong meditation practice, but this was a real challenge.

I've given up on a lot of people who will not listen. Maybe I'm more aware of it, but it seems to be an increasing problem everywhere. I don't know why it seems so much more noticeable here.




Eh, I just got the first time jitters. lol

I've been reading all these grow logs and such, and a lot of them would have a spot about half the size of a dime by now, yet I've got nothing. I guess it just takes longer for some people. I read plenty that had a full dime sized spot on day 4, and I was just hoping that would happen to me. Guess not. Oh well, its not a big deal to me at all. Plus I wanted to ask about those temp changes I was doing.

I would like to know though, when I should be concened. For example, should I be worried if I see nothing after 8 days?


Edited by bennylava (09/07/15 09:18 AM)


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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,342
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Re: Nothing after 3 days? [Re: bennylava]
    #22201142 - 09/07/15 09:17 AM (8 years, 4 months ago)

mycelium growth generates heat. if its reading 86 externally, its going to be 90+ internally, if it starts growing. very bad idea to keep the room temperatures this high.

"high temps" and "incubation" like this only speed up colonization by like a day, tops, at the risk of germinating contaminates. its so not worth it.


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InvisibleShroomerInTheRye
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I'm a teapot User Gallery


Registered: 01/12/12
Posts: 13,036
Loc: Themyscira Flag
Re: Nothing after 3 days? [Re: bennylava]
    #22201188 - 09/07/15 09:33 AM (8 years, 4 months ago)

In my first grow, it took 2 weeks for me to see any kind of growth.


--------------------
:nyan: <-- Clicky Clicky


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OfflineIlyana
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Registered: 02/04/14
Posts: 50
Last seen: 8 years, 2 months
Re: Nothing after 3 days? [Re: ShroomerInTheRye]
    #22201230 - 09/07/15 09:46 AM (8 years, 4 months ago)

I just started a new grow (old skool PF tek) using a Mazatapec syringe that was 18 months old and after 8 days I'm only now seeing some mycelium growth on a *few* of my jars.


BE PATIENT!


also, grass seed tek but you used brf cakes? wtf huh???


--------------------
read, read, and read some more!





Mushrooms & Stone Mandala


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Offlinespacechildo
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Registered: 01/24/13
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Re: Nothing after 3 days? [Re: Munchauzen]
    #22201252 - 09/07/15 09:53 AM (8 years, 4 months ago)

Quote:

Munchauzen said:
"high temps" and "incubation" like this only speed up colonization by like a day, tops, at the risk of germinating contaminates. its so not worth it.




if you go over 80F you see mycel starting to slow down. its not higher = faster.
optimal = faster. but optimal for cubes is also within range for many contaminants.

so we lower the temps just a tad, cube myc grows fast and contams dont wanna come out and play.

I know you know this munch, I'm just emphazising it to the OP and that other dude.


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InvisibleMunchauzen
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Re: Nothing after 3 days? [Re: spacechildo]
    #22201279 - 09/07/15 10:00 AM (8 years, 4 months ago)

Quote:

spacechildo said:
Quote:

Munchauzen said:
"high temps" and "incubation" like this only speed up colonization by like a day, tops, at the risk of germinating contaminates. its so not worth it.




if you go over 80F you see mycel starting to slow down. its not higher = faster.
optimal = faster. but optimal for cubes is also within range for many contaminants.

so we lower the temps just a tad, cube myc grows fast and contams dont wanna come out and play.

I know you know this munch, I'm just emphazising it to the OP and that other dude.




:whathesaid:

yeah i should have been more clear i wouldnt go near 80. I accidentally left my a/c off one day this year and when I noticed the thermostat it was 80. I ended up with 40 quarts of inoculuated oats all stop growing and go bacterial, and 50 pasty plates do the exact same. it ruined my entire grow, and I pretty much lost all the magnificent cultures I had acquired. I tried saving them, but was only able to save APE it would seem. I've never had anything like that happen all at once, so it was pretty devastating. Set me back many weeks worth of work.


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Offlinebennylava
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Registered: 05/29/15
Posts: 587
Last seen: 6 years, 9 months
Re: Nothing after 3 days? [Re: Munchauzen]
    #22201318 - 09/07/15 10:09 AM (8 years, 4 months ago)

Ok I put them back in the closet where they'll only see about 76-78 degrees. Wouldn't the mycilium eat the contams, once it got to them?

Also to answer somebodys question, I just used BRF in the grass seed tek cause that's what she said to do for complete noobs. In the guide. Plus I didn't know what species of grass seed to get. Well I mean I knew it was rye, but you got winter rye, perennial, anual, and I think one other. Maybe it doesn't matter? Don't know.


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InvisibleShroomerInTheRye
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Registered: 01/12/12
Posts: 13,036
Loc: Themyscira Flag
Re: Nothing after 3 days? [Re: bennylava]
    #22201333 - 09/07/15 10:14 AM (8 years, 4 months ago)

The myc will grow over the contams eventually, but if you put the cake in a fruiting chamber with other healthy cakes, it would be bad news.  The contamination would most likely spread to the other, healthy cakes.

Think about it like if you got a staph infection.  It wouldn't go away on its own.  You'd need antibiotics.


--------------------
:nyan: <-- Clicky Clicky


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InvisibleMunchauzen
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Re: Nothing after 3 days? [Re: bennylava]
    #22201339 - 09/07/15 10:18 AM (8 years, 4 months ago)

it will just envelop them. not consume them. they are still present.


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Offlinebennylava
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Registered: 05/29/15
Posts: 587
Last seen: 6 years, 9 months
Re: Nothing after 3 days? [Re: Munchauzen]
    #22222603 - 09/11/15 03:30 PM (8 years, 4 months ago)

Ok so 7 days now, still nothing. Someone said it could take up to 14 days to even start to see anything at all.

So until then, I'll just ask what causes them NOT to grow. Does anyone ever come in here and say "Well, nothing grew". And what are the primary causes of that? Perhaps I can avoid it if I know what causes the myc to never grow.


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InvisibleShroomerInTheRye
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Registered: 01/12/12
Posts: 13,036
Loc: Themyscira Flag
Re: Nothing after 3 days? [Re: bennylava]
    #22223037 - 09/11/15 05:07 PM (8 years, 4 months ago)

Improper moisture content and non-viable spores are the first things that pop into my head.  Contaminants also.

Here's a silly question...do you still have the foil on your jars?


--------------------
:nyan: <-- Clicky Clicky


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