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Sivarted
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Registered: 08/22/15
Posts: 1,084
Last seen: 2 months, 10 days
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Drop spores directly into sterile water vs spore print
#22199492 - 09/06/15 08:59 PM (8 years, 4 months ago) |
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So I have a spore print that I made from my very first grow mushroom, done in a SAB on foil, and my obvious goal is to never need to buy spores of this particular strain again.
I'm concerned about sterility of the print though. I've watched RR's videos, and read a ton about spore syringes, and what I'm really wondering is what the contamination danger is of letting a cap drop spores directly into sterilized water because that seems like the simplest way to make new syringes with the fewest steps possible.
However, I've read threads that said dropping directly into sterilized water is a great way to go, and threads that say using the entire print (which is essentially what you're doing when dropping directly into water) is guaranteed to be contaminated because the cap was grown in open air and *will* have contaminants that drop into the water with the mushroom spores, and that the reason making a syringe from a print works is that you use the inside of the print that reduces the chances of contaminants, and that only using a small section of the print further reduces the odds of contamination.
With so much directly conflicting info it's difficult to know exactly how to proceed, whether I should use my existing print the normal way, or drop directly into a jar, which would give me a simple and easy to use spore syringe supply.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Drop spores directly into sterile water vs spore print [Re: Sivarted]
#22199812 - 09/06/15 10:05 PM (8 years, 4 months ago) |
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if your goal is to never buy spores again, then you definitely want to make a print... you can make 100's of syringes from a single print.
of course you should only use as little spores as possible and it sounds like you know why
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 11 days, 53 minutes
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Re: Drop spores directly into sterile water vs spore print [Re: Sivarted]
#22199836 - 09/06/15 10:11 PM (8 years, 4 months ago) |
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Do you use agar? This would be prefered, to swab some spores onto agar, then you could get rid of any contaminates. To make a spore syringe from a print you'll want to use a water filled sterilized syringe and fill a clean, sterlized glass, like a shot glass, while working in your SAB. Then while using a sterilized utensil, you'll want to drop spores in water, or if you can drop straight from the foil, then suck water, back into syringe. You can't always guarantee a contaminate free syringe from MS print.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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Sivarted
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Registered: 08/22/15
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Re: Drop spores directly into sterile water vs spore print [Re: Munchauzen]
#22199847 - 09/06/15 10:14 PM (8 years, 4 months ago) |
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Well, simplicity and removing possible contamination steps is also important to me, and I can very easily make new prints, the mushrooms themselves are obviously grown in open air though.
But if I could safely have a cap drop spores into, say, a pint jar of sterilized water, that would last me a long-ass time, and I could save prints on foil for storage and potential future use.
I also plan to get into agar and isolates and culture slants at some point, but printing is something that I can do right now, with the materials at hand.
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Re: Drop spores directly into sterile water vs spore print [Re: Sivarted]
#22199868 - 09/06/15 10:19 PM (8 years, 4 months ago) |
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Quote:
Sivarted said: Well, simplicity and removing possible contamination steps is also important to me, and I can very easily make new prints, the mushrooms themselves are obviously grown in open air though.
But if I could safely have a cap drop spores into, say, a pint jar of sterilized water, that would last me a long-ass time, and I could save prints on foil for storage and potential future use.
I also plan to get into agar and isolates and culture slants at some point, but printing is something that I can do right now, with the materials at hand. 
If you have a PC, you have all you need to do agar. But I completely understand if you're not willing to make the jump yet. I agree with Munch that a safer and better alternative is probably to go straight to a print, let it dry and then scrape some of the spores into some sterilized water. There are a few reasons for this: it's a lot harder for something to go wrong, it's easier to store, and drying out your spores kills off almost all of the non-endospore producing bacterial nasties.
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Sivarted
Stranger
Registered: 08/22/15
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Re: Drop spores directly into sterile water vs spore print [Re: Psilicon]
#22199907 - 09/06/15 10:27 PM (8 years, 4 months ago) |
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Quote:
van der griegen said:
If you have a PC, you have all you need to do agar. But I completely understand if you're not willing to make the jump yet. I agree with Munch that a safer and better alternative is probably to go straight to a print, let it dry and then scrape some of the spores into some sterilized water. There are a few reasons for this: it's a lot harder for something to go wrong, it's easier to store, and drying out your spores kills off almost all of the non-endospore producing bacterial nasties.
I do have a PC but money is *super* tight right now because I'm putting every spare penny into helping my wife and daughter start up a bakery, so I don't really even have funds to buy agar plates and scalpel and whatnot even though I realize they're super cheap. Brown rice flour and vermiculite have to take precedence since I'm currently doing pftek.
Everything that's been said makes a lot of sense though, so I'll just start making prints in my SAB from the larger ones. (I've had 5 or 6 so far that weighed around or more than an ounce fresh with huge caps, and I'm just barely starting second flush on my first grow)
Thanks much.
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 11 days, 53 minutes
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Re: Drop spores directly into sterile water vs spore print [Re: Sivarted]
#22199925 - 09/06/15 10:31 PM (8 years, 4 months ago) |
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Yea just make those prints and hang on to them. Family comes first.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Re: Drop spores directly into sterile water vs spore print [Re: Sivarted]
#22199930 - 09/06/15 10:32 PM (8 years, 4 months ago) |
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Quote:
Sivarted said:
I do have a PC but money is *super* tight right now because I'm putting every spare penny into helping my wife and daughter start up a bakery, so I don't really even have funds to buy agar plates and scalpel and whatnot even though I realize they're super cheap. Brown rice flour and vermiculite have to take precedence since I'm currently doing pftek.
Everything that's been said makes a lot of sense though, so I'll just start making prints in my SAB from the larger ones. (I've had 5 or 6 so far that weighed around or more than an ounce fresh with huge caps, and I'm just barely starting second flush on my first grow)
Thanks much. 

If you have a newer variety, remember not to select for spore production, but instead for potency, fruiting and colonization!
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Drop spores directly into sterile water vs spore print [Re: Sivarted]
#22199932 - 09/06/15 10:32 PM (8 years, 4 months ago) |
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if you have a coffee bean grinder, you can save some $$ buy buying short grain brown rice and grinding it yourself into brown rice flour. thats all it is. the rice is usually like .99 cents a pound, where as the brf is like 4.99 a pound!!
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Re: Drop spores directly into sterile water vs spore print [Re: Munchauzen]
#22199935 - 09/06/15 10:33 PM (8 years, 4 months ago) |
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Quote:
Munchauzen said: if you have a coffee bean grinder, you can save some $$ buy buying short grain brown rice and grinding it yourself into brown rice flour. thats all it is. the rice is usually like .99 cents a pound, where as the brf is like 4.99 a pound!!
I prefer working with the rougher ground coffee grinder shit, too.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Drop spores directly into sterile water vs spore print [Re: Psilicon]
#22199998 - 09/06/15 10:59 PM (8 years, 4 months ago) |
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Quote:
van der griegen said:
Quote:
Sivarted said:
I do have a PC but money is *super* tight right now because I'm putting every spare penny into helping my wife and daughter start up a bakery, so I don't really even have funds to buy agar plates and scalpel and whatnot even though I realize they're super cheap. Brown rice flour and vermiculite have to take precedence since I'm currently doing pftek.
Everything that's been said makes a lot of sense though, so I'll just start making prints in my SAB from the larger ones. (I've had 5 or 6 so far that weighed around or more than an ounce fresh with huge caps, and I'm just barely starting second flush on my first grow)
Thanks much. 

If you have a newer variety, remember not to select for spore production, but instead for potency, fruiting and colonization!

That sounds like your saying "cubes aren't cubes" 
Good to see ya round van
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Re: Drop spores directly into sterile water vs spore print [Re: Pastywhyte]
#22200168 - 09/07/15 12:09 AM (8 years, 4 months ago) |
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Quote:
Pastywhyte said:]

That sounds like your saying "cubes aren't cubes" 
Good to see ya round van

Yeah, you've got like a 95% chance your genetics have been fucked by some idiot in the past. But hey, one in twenty people are part of the other 5%.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Drop spores directly into sterile water vs spore print [Re: Psilicon]
#22200175 - 09/07/15 12:11 AM (8 years, 4 months ago) |
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Lol too true. Good times on the horizon tho. Very exciting stuff being done by lots of people.
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