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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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How to make a perfect spore syringe
#22198465 - 09/06/15 05:56 PM (8 years, 4 months ago) |
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okay so i have on hand materials Sterile syringes Presumably clean prints Pc SAB
But im lacking a small glass to drop spores into any ideas ?
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Stuey1
We're all mad Here



Registered: 11/28/13
Posts: 740
Loc: Bat Country
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Re: How to make a perfect spore syringe [Re: mrbart4444]
#22198533 - 09/06/15 06:09 PM (8 years, 4 months ago) |
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Do you have a shotglass?
-------------------- Have you ever danced with the devil in the pale moon light?
 
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dinosaurcocks
Stranger

Registered: 08/14/15
Posts: 24
Last seen: 8 years, 1 month
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Re: How to make a perfect spore syringe [Re: Stuey1]
#22198731 - 09/06/15 06:54 PM (8 years, 4 months ago) |
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i bought a few little flower vases from walmart, in the section with plastic flowers, they were a buck a piece, probably about 50cc, and shaped pretty much like a Erlenmeyer flask, i looked everywhere for an actual erlenmeyer flask, but couldn't find any smaller than 1000 cc's...the wide base and small neck make for a perfect substitute though, and they seem to hold up in the pc just fine... looks pretty much like this, and is about 4 inches tall...
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: How to make a perfect spore syringe [Re: Stuey1]
#22198796 - 09/06/15 07:10 PM (8 years, 4 months ago) |
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Quote:
Stuey1 said: Do you have a shotglass?
no i dont
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: How to make a perfect spore syringe [Re: mrbart4444]
#22198886 - 09/06/15 07:23 PM (8 years, 4 months ago) |
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Do you have a walmart near you?
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: How to make a perfect spore syringe [Re: TheEaglesGift]
#22198973 - 09/06/15 07:32 PM (8 years, 4 months ago) |
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Quote:
TheEaglesGift said: Do you have a walmart near you? 
yea i guess im going to make a trip just tried of buying shit
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: How to make a perfect spore syringe [Re: mrbart4444]
#22198999 - 09/06/15 07:35 PM (8 years, 4 months ago) |
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I hear you on that, buddy.
Luckily, whatever container you buy you can reuse indefinitely.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: How to make a perfect spore syringe [Re: TheEaglesGift]
#22199629 - 09/06/15 09:26 PM (8 years, 4 months ago) |
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If you have any sterile petri dishes I've used those successfully. Don't know if its cost effective though lol. 1/2 pint jars work pretty good. A short drinking glass. Pretty much any glass will work.
-------------------- Trade List πππ 6 hole Mini Monos
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: How to make a perfect spore syringe [Re: TheEaglesGift]
#22199659 - 09/06/15 09:32 PM (8 years, 4 months ago) |
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went to the store got one of these 
what technique do you use when creating a spore syringe
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: How to make a perfect spore syringe [Re: mrbart4444]
#22199679 - 09/06/15 09:36 PM (8 years, 4 months ago) |
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Quote:
If you have any sterile petri dishes I've used those successfully
i am a fan of these asβ¦wellβ¦...
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: How to make a perfect spore syringe [Re: blindingleaf]
#22199720 - 09/06/15 09:42 PM (8 years, 4 months ago) |
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I pc a jar of water with a ship in the lid, let it cool and bring it in the sab. Use the need needle of the syinge to scrape the spores into the clean shot glass, squirt a little water into the glass, stir it with the needle and suck it up.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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You may have to squirt some water from the syringe back into to the glass and suck it back up a couple times to get all the spores into the syringe
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Anchor makes some killer glassware in general. They're a great company. USA made.
Wasn't aware they made "shot glasses", cause that is what that is!
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Quote:
PinPornProducer said: I pc a jar of water with a ship in the lid, let it cool and bring it in the sab. Use the need needle of the syinge to scrape the spores into the clean shot glass, squirt a little water into the glass, stir it with the needle and suck it up.
Most spore syringes are like 15-20 a pop so maybe cost effective you look at it like that do i need to sterilize the glass first? i was thinking of putting the glass in quart jar and let it pc alone with the glass containing the water?
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: How to make a perfect spore syringe [Re: mrbart4444]
#22199883 - 09/06/15 10:21 PM (8 years, 4 months ago) |
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Sterilizing the jar would be a good idea, but maybe overkill. Boiling water or an iso wash would probably be sufficient. You have to understand that your spore print is almost certainly not sterile anyways. Sometimes overkill is good, it depends on what you want to do with the syringe.
I take all of my spores(print or syringe) to agar first anyways, so I can clean them up in the event that contamination presents.
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 11 days, 3 hours
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Re: How to make a perfect spore syringe [Re: mrbart4444]
#22199886 - 09/06/15 10:22 PM (8 years, 4 months ago) |
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Yes you want to use a sterilized glass. Either wrap in foil and PC, or just hand wash with soap and water, then bring into SAB and wipe with 70% iso.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks βThe probability of success is difficult to estimate; but if we never search the chance of success is zero.β -Giuseppe Cocconi
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: How to make a perfect spore syringe [Re: iSmkGrnBud]
#22199891 - 09/06/15 10:24 PM (8 years, 4 months ago) |
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Wiping with 70 percent iso will not sterilize it, only sanitize it. Big difference.
I'm not nit picking you for the fun of it, but correct terminology in this hobby is important so we all are on the same page.
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 11 days, 3 hours
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Re: How to make a perfect spore syringe [Re: TheEaglesGift]
#22199901 - 09/06/15 10:26 PM (8 years, 4 months ago) |
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I thought we always use 70% iso because it permeates the cells more, where as anything higher is too volatile and evaporates too quickly? 
It's all good. I'd like to be pointed out when my terminology or advice isn't correct. I'm here to learn.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks βThe probability of success is difficult to estimate; but if we never search the chance of success is zero.β -Giuseppe Cocconi
Edited by iSmkGrnBud (09/06/15 10:29 PM)
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: How to make a perfect spore syringe [Re: iSmkGrnBud]
#22199952 - 09/06/15 10:37 PM (8 years, 4 months ago) |
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70s percent is better than 90 percent for the reason you stated, but it still doesn't sterilize.
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 11 days, 3 hours
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Re: How to make a perfect spore syringe [Re: TheEaglesGift]
#22199966 - 09/06/15 10:42 PM (8 years, 4 months ago) |
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Quote:
TheEaglesGift said: 70s percent is better than 90 percent for the reason you stated, but it still doesn't sterilize.
Thanks for the clarification.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks βThe probability of success is difficult to estimate; but if we never search the chance of success is zero.β -Giuseppe Cocconi
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: How to make a perfect spore syringe [Re: iSmkGrnBud]
#22199985 - 09/06/15 10:53 PM (8 years, 4 months ago) |
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