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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
How to make a perfect spore syringe
    #22198465 - 09/06/15 05:56 PM (8 years, 4 months ago)

okay so i have on hand materials
Sterile syringes
Presumably clean prints
Pc
SAB

But im lacking a small glass to drop spores into
any ideas ?


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InvisibleStuey1
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Registered: 11/28/13
Posts: 740
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Re: How to make a perfect spore syringe [Re: mrbart4444]
    #22198533 - 09/06/15 06:09 PM (8 years, 4 months ago)

Do you have a shotglass?


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Offlinedinosaurcocks
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Re: How to make a perfect spore syringe [Re: Stuey1]
    #22198731 - 09/06/15 06:54 PM (8 years, 4 months ago)

i bought a few little flower vases from walmart, in the section with plastic flowers, they were a buck a piece, probably about 50cc, and shaped pretty much like a Erlenmeyer flask, i looked everywhere for an actual erlenmeyer flask, but couldn't find any smaller than 1000 cc's...the wide base and small neck make for a perfect substitute though, and they seem to hold up in the pc just fine... looks pretty much like this, and is about 4 inches tall...


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: How to make a perfect spore syringe [Re: Stuey1]
    #22198796 - 09/06/15 07:10 PM (8 years, 4 months ago)

Quote:

Stuey1 said:
Do you have a shotglass?



no i dont


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: How to make a perfect spore syringe [Re: mrbart4444]
    #22198886 - 09/06/15 07:23 PM (8 years, 4 months ago)

Do you have a walmart near you? :lol:


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: How to make a perfect spore syringe [Re: TheEaglesGift]
    #22198973 - 09/06/15 07:32 PM (8 years, 4 months ago)

Quote:

TheEaglesGift said:
Do you have a walmart near you? :lol:



yea i guess im going to make a trip just tried of buying shit


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: How to make a perfect spore syringe [Re: mrbart4444]
    #22198999 - 09/06/15 07:35 PM (8 years, 4 months ago)

I hear you on that, buddy.

Luckily, whatever container you buy you can reuse indefinitely.


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Invisiblegizmo1
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Registered: 06/15/11
Posts: 3,831
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Re: How to make a perfect spore syringe [Re: TheEaglesGift]
    #22199629 - 09/06/15 09:26 PM (8 years, 4 months ago)

If you have any sterile petri dishes I've used those successfully. Don't know if its cost effective though lol. 1/2 pint jars work pretty good. A short drinking glass. Pretty much any glass will work.


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: How to make a perfect spore syringe [Re: TheEaglesGift]
    #22199659 - 09/06/15 09:32 PM (8 years, 4 months ago)

went to the store got one of these

what technique do you use when creating a spore syringe


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Invisibleblindingleaf
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Re: How to make a perfect spore syringe [Re: mrbart4444]
    #22199679 - 09/06/15 09:36 PM (8 years, 4 months ago)

Quote:

If you have any sterile petri dishes I've used those successfully




i am a fan of these as…well…...:notsureif:


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OfflinePinPornProducer
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Re: How to make a perfect spore syringe [Re: blindingleaf]
    #22199720 - 09/06/15 09:42 PM (8 years, 4 months ago)

I pc a jar of water with a ship in the lid, let it cool and bring it in the sab. Use the need needle of the syinge to scrape the spores into the clean shot glass, squirt a little water into the glass, stir it with the needle and suck it up.


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OfflinePinPornProducer
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Re: How to make a perfect spore syringe [Re: PinPornProducer]
    #22199727 - 09/06/15 09:44 PM (8 years, 4 months ago)

You may have to squirt some water from the syringe back into to the glass and suck it back up a couple times to get all the spores into the syringe


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: How to make a perfect spore syringe [Re: PinPornProducer]
    #22199780 - 09/06/15 09:56 PM (8 years, 4 months ago)

Anchor makes some killer glassware in general. They're a great company. USA made.

Wasn't aware they made "shot glasses", cause that is what that is!


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: How to make a perfect spore syringe [Re: PinPornProducer]
    #22199867 - 09/06/15 10:19 PM (8 years, 4 months ago)

Quote:

PinPornProducer said:
I pc a jar of water with a ship in the lid, let it cool and bring it in the sab. Use the need needle of the syinge to scrape the spores into the clean shot glass, squirt a little water into the glass, stir it with the needle and suck it up.



Most spore syringes are like 15-20 a pop so maybe cost effective you look at it like that
do i need to sterilize the glass first? i was thinking of putting the glass in quart jar and let it pc alone with the glass containing the water?


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: How to make a perfect spore syringe [Re: mrbart4444]
    #22199883 - 09/06/15 10:21 PM (8 years, 4 months ago)

Sterilizing the jar would be a good idea, but maybe overkill. Boiling water or an iso wash would probably be sufficient. You have to understand that your spore print is almost certainly not sterile anyways. Sometimes overkill is good, it depends on what you want to do with the syringe.

I take all of my spores(print or syringe) to agar first anyways, so I can clean them up in the event that contamination presents.


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OfflineiSmkGrnBud
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Re: How to make a perfect spore syringe [Re: mrbart4444]
    #22199886 - 09/06/15 10:22 PM (8 years, 4 months ago)

Yes you want to use a sterilized glass. Either wrap in foil and PC, or just hand wash with soap and water, then bring into SAB and wipe with 70% iso.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
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Loc: Ixtlan, Mexico
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Re: How to make a perfect spore syringe [Re: iSmkGrnBud]
    #22199891 - 09/06/15 10:24 PM (8 years, 4 months ago)

Wiping with 70 percent iso will not sterilize it, only sanitize it. Big difference.

I'm not nit picking you for the fun of it, but correct terminology in this hobby is important so we all are on the same page. :thumbup:


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OfflineiSmkGrnBud
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Re: How to make a perfect spore syringe [Re: TheEaglesGift]
    #22199901 - 09/06/15 10:26 PM (8 years, 4 months ago)

I thought we always use 70% iso because it permeates the cells more, where as anything higher is too volatile and evaporates too quickly? :shrug:

It's all good. I'd like to be pointed out when my terminology or advice isn't correct. I'm here to learn.


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Edited by iSmkGrnBud (09/06/15 10:29 PM)


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: How to make a perfect spore syringe [Re: iSmkGrnBud]
    #22199952 - 09/06/15 10:37 PM (8 years, 4 months ago)

70s percent is better than 90 percent for the reason you stated, but it still doesn't sterilize.


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OfflineiSmkGrnBud
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Re: How to make a perfect spore syringe [Re: TheEaglesGift]
    #22199966 - 09/06/15 10:42 PM (8 years, 4 months ago)

Quote:

TheEaglesGift said:
70s percent is better than 90 percent for the reason you stated, but it still doesn't sterilize.




:cheers: Thanks for the clarification.


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