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Kalistis

Registered: 09/06/15
Posts: 2,265
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Autoclave Question
#22198290 - 09/06/15 05:13 PM (8 years, 4 months ago) |
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Out of curiosity, does anyone have any experience using a medical grade autoclave (one used to sterilize surgical instruments) for the purpose of sterilizing their substrate? If so, at what temp, PSI, and time do you set your autoclave for? My primary concern is that I may overcook my substrate and lose nutrients in the process.
It appears I can fit about 8 half pint jars in my autoclave without using the shelf and 16 if I use the shelf.
Any advice would be appreciated.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Autoclave Question [Re: Kalistis]
#22198486 - 09/06/15 06:01 PM (8 years, 4 months ago) |
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If you want to sterilize your brf jars then do 60-90 minutes at 15 psi (250f).
If you want to sterilize grains do 90 minutes at 15 psi (250f).
And can you post a pic of the autoclave you are using? It should work fine for sterilizing substrates.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Make sure its set to sterilize media, not dry items. And it's the same 90+ min at 15psi
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Kalistis

Registered: 09/06/15
Posts: 2,265
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Thanks for the responses. I'm not currently where I keep the autoclave, but I did find some images on my phone that I took of the gauges back when I was attempting to sell the unit. Somehow I misplaced the picture of the entire unit, but I did find a similar model online for reference.
My unit:



And the closest I could find online:
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Kalistis

Registered: 09/06/15
Posts: 2,265
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Quote:
bodhisatta said: Make sure its set to sterilize media, not dry items. And it's the same 90+ min at 15psi
I think the only difference would be that I would set my temp and PSI lower than if I was sterilizing surgical instruments or dry packs. This old school autoclave uses a reservoir tank of distilled water that fills a pan which would sit below the substrate. It works by heating up the water that was released from the tank into the main chamber, creating the steam and pressure. It is incredibly accurate and reliable for what I frequently use it for, but then I have never sterilized substrate in it. Plus, I wouldn't have to invest in another autoclave unless you guys felt this won't work.
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Re: Autoclave Question [Re: Kalistis]
#22199890 - 09/06/15 10:23 PM (8 years, 4 months ago) |
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Quote:
Caelistis said:
Quote:
bodhisatta said: Make sure its set to sterilize media, not dry items. And it's the same 90+ min at 15psi
I think the only difference would be that I would set my temp and PSI lower than if I was sterilizing surgical instruments or dry packs. This old school autoclave uses a reservoir tank of distilled water that fills a pan which would sit below the substrate. It works by heating up the water that was released from the tank into the main chamber, creating the steam and pressure. It is incredibly accurate and reliable for what I frequently use it for, but then I have never sterilized substrate in it. Plus, I wouldn't have to invest in another autoclave unless you guys felt this won't work.
I sincerely doubt that your autoclave is too powerful for this purpose. 
What kind of temperature and pressure do you typically use?
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Kalistis

Registered: 09/06/15
Posts: 2,265
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Re: Autoclave Question [Re: Psilicon]
#22200145 - 09/07/15 12:01 AM (8 years, 4 months ago) |
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Surgical packs 265 degrees at 20 to 30 PSI for 1 hour, but I have flash sterilized items at higher temps and PSI for much shorter times. I'm certain I can also lower temps and PSI even though I haven't yet tried.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Autoclave Question [Re: Kalistis]
#22201103 - 09/07/15 09:03 AM (8 years, 4 months ago) |
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For dry items the temp is usually set to 260+
As well as enabling the pump at the end of the cycle to create a vacuum and so the dry instruments come out dry.
Wet cycles are allowed to slow exhaust so you don't get boil over
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Kalistis

Registered: 09/06/15
Posts: 2,265
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Yeah, this autoclave doesn't have a pump. Items will come out moist if I put too much water in the pan or don't get high enough temps. This autoclave also won't boil over. It's not possible. So, I'm good?
I may give it a few tries and see how it goes. Thanks guys.
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