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Invisiblem1nd6am3sk8er
Registered: 02/10/08
Posts: 35
Help with a couple of questions about sterilizing agar.
    #22192490 - 09/05/15 12:36 PM (8 years, 4 months ago)

So I'm using Pasty's agar tek and following it to a T.  But I also have some petri dishes and want to do a pour.

So for the pour, my plan is to sterilize agar in a glass jar.  The Pace Picante salsa jars.  My question is how should I prepare the lid?  I'm thinking of drilling a hole and stuffing it with polyfill.  Is that legitimate?  If so, I plan on sterilizing the same amount of time as the Pasty agar.  Is that also okay?

If there is a link to a tek for sterilizing agar for a pour can someone post it?

Thanks a bunch.


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OfflineCliftonGK1
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Registered: 03/27/13
Posts: 327
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Re: Help with a couple of questions about sterilizing agar. [Re: m1nd6am3sk8er]
    #22192543 - 09/05/15 12:46 PM (8 years, 4 months ago)

I use regular mouth pint jars, fill them 1/3 to avoid boil over, and use a regular lid with a polyfil stuffed 1/4" hole for ventilation.  I cover with foil for the PC cycle, and I remove the foil only after the jars have cooled, I've sprayed them with EtOH, and transferred them into my SAB along with my other materials.


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