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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22188806 - 09/04/15 04:13 PM (8 years, 5 months ago)

:lol: :onfire: :lol:


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OfflineAjahn Don
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #22188912 - 09/04/15 04:41 PM (8 years, 5 months ago)

Quote:

Pastywhyte said:
Quote:

Ajahn Don said:
I wish someone had warned us about the smell. Ok, warned me. The missus-after-seven-o'clock tonight is not happy about it. Oat water agar is not on the list of options anymore.

Still a hundred degrees. Not opening the windows.




Wait, what smell? Grain water agar should make less smell than PCing the grains :shrug:




I boil the agar for 5 minutes to thicken it. Bringing the oat grain water up to a boil was smelly. In the PC now, so I have no idea what it's going to look like. Tried a new agar. It came in bags and was strings like pasta. I added red food coloring in honor of my alma mater, the Univ of Arkansas Razorbacks. Go Hogs!

Woo pig, sooie!

:biggrin:


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"He's not altogether dense, but he's not altogether there."


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Ajahn Don]
    #22189042 - 09/04/15 05:11 PM (8 years, 5 months ago)

My grain water didn't smell at all last night. I thawed it by placing the Tupperware on top of my meat rack, resting on top of a boiling pot of water. I strained the thick goop via paper towel until it was clear liquid. No smell at all. Then I did Pasty's Tek to a T and again, no smell. :undecided:


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Invisibleblackdust
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22189058 - 09/04/15 05:13 PM (8 years, 5 months ago)

Have you tried using a coffee filter?


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Invisibleeatyualive
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Re: Pasty Agar Tek [Re: blackdust]
    #22189111 - 09/04/15 05:28 PM (8 years, 5 months ago)

Cornmeal pasty plates. And the consistency is pasty(no pun intended)



:bananadance:


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EAT GETS SHIT DONE


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: blackdust]
    #22189143 - 09/04/15 05:35 PM (8 years, 5 months ago)

Don't drink coffee so I have none. Only have a coffee grinder for agar. :lol:


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Invisibleblackdust
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22189159 - 09/04/15 05:37 PM (8 years, 5 months ago)

Gotcha. I dont drink coffee either but had to pick some up yesterday for filtering dmt crystals


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: blackdust]
    #22189598 - 09/04/15 07:05 PM (8 years, 5 months ago)

I've been using 2-3 year old holiday flavored coffee in all my subs. :lol:

and yeah my oat water doesn't have a smell either.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflinePsilosoulful

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Re: Pasty Agar Tek [Re: Inocuole]
    #22189647 - 09/04/15 07:10 PM (8 years, 5 months ago)

Quote:

Inocuole said:
I've been using 2-3 year old holiday flavored coffee in all my subs. :lol:



:devito:


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: Psilosoulful]
    #22189674 - 09/04/15 07:14 PM (8 years, 5 months ago)

Makes my substrate smell like the season for giving.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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OfflineDistrict
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Re: Pasty Agar Tek [Re: Inocuole]
    #22190789 - 09/04/15 10:42 PM (8 years, 5 months ago)

For condensation, it seems to have been defeated by stacking the plates and placing hot water on the top most plate.

I don't have access to glad mini here, and so the plates, especially the lid tightness, are questionable. I'll be cooling them in a moist SAB with holes covered to reduce that vacuum contamination. Condensation becomes a vector when excess water breeds bacteria(?). I'm cloning wild.


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Offlinenewera
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Re: Pasty Agar Tek [Re: District]
    #22191204 - 09/05/15 01:24 AM (8 years, 5 months ago)

bacteria has to be there for it to breed though so it's mainly a problem with spores/technique


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: newera]
    #22191242 - 09/05/15 01:45 AM (8 years, 5 months ago)

How much do you guys spray down your SAB before using? I usually spray Lysol on the walls, wipe them down to clean shit, and then soapy water around the sides (not the top because I can't see if I do). Then I shower and come out and use it after 20m or so. Any issues with that?


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Offlinenewera
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22191249 - 09/05/15 01:50 AM (8 years, 5 months ago)

I always see people posting that they wait 20+ minutes but i swear I've read RR post you only need to wait a few seconds for the mist to settle, that is what I've always done ...maybe i'm doing ti wrong though i'm no pro and I've had my share of hurdles


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: newera]
    #22191275 - 09/05/15 02:14 AM (8 years, 5 months ago)

Yeah, I often come out of the shower and then spray down and use it ASAP. The 20 is just because I'm showering, not for any logical reasoning.

I just drilled a 1/4" hole into the top of SAB so I can take good pictures of cultures in there. I figure the phone will block contaminants.


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InvisibleBuck513

Registered: 04/17/14
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22191315 - 09/05/15 03:00 AM (8 years, 5 months ago)

Quote:

stareatclouds said:
How much do you guys spray down your SAB before using? I usually spray Lysol on the walls, wipe them down to clean shit, and then soapy water around the sides (not the top because I can't see if I do). Then I shower and come out and use it after 20m or so. Any issues with that?



I just get a paper towel moist with alcohol, give it a good wipe down, set everything up, mist, then immediately get to work.


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Buck513]
    #22191439 - 09/05/15 05:16 AM (8 years, 5 months ago)

Word. Man, I won't lie, I fucking hate agar work. My SAB sucks, I can never EVER get a chunk of myc out in one piece, my lids are being shitty, I hate wearing the suit, etc. Just made 2 of the worst LI's ever because I couldn't get the agar chunk out in one piece. Hope they're still viable. But whatever, this is agar.

Anyway, used my loop to put spores on a bunch of plates. Got Golden Teacher, AA+, PF Classic on about 3 plates each. Also made 3 more transfers from my iffy PE plate as well.

Here's a shot of one of the plates through my new peekaboo hole in the lid.



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InvisibleInocuole
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22191452 - 09/05/15 05:28 AM (8 years, 5 months ago)

You wear a suit?  Heh...  I did some agar barehanded with no gloves the other day, I don't remember which though.. guess I'll see.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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InvisiblePanda89
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Re: Pasty Agar Tek [Re: Inocuole]
    #22191923 - 09/05/15 09:33 AM (8 years, 5 months ago)

Could I use ez felt to cover the holes instead of micro pore tape? Can any container be used as long as it is made from pp5 plastic?


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Inocuole]
    #22192424 - 09/05/15 12:22 PM (8 years, 5 months ago)

I just meant the gloves and mask and shit. I wear a thin sweatshirt with my hood on, gloves and mask.


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