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OfflineSchism462
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Registered: 07/08/15
Posts: 23
Last seen: 8 years, 3 months
IS THIS TRICH?! *DELETED*
    #22191918 - 09/05/15 09:31 AM (8 years, 4 months ago)

Post deleted by Schism462

Reason for deletion: K



Edited by Schism462 (09/05/15 09:33 AM)


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OfflinePinPornProducer
Buy the ticket, take the ride
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Registered: 08/23/14
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Re: iS THIS TRICH? [Re: Schism462]
    #22191921 - 09/05/15 09:32 AM (8 years, 4 months ago)

Yes sir, the white growing over the green in more trich


Edited by PinPornProducer (09/05/15 09:33 AM)


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OfflineSchism462
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Registered: 07/08/15
Posts: 23
Last seen: 8 years, 3 months
Re: iS THIS TRICH? [Re: PinPornProducer]
    #22191953 - 09/05/15 09:42 AM (8 years, 4 months ago)

Damn, that's what I was afraid of.  Thanks.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: iS THIS TRICH? [Re: Schism462]
    #22194035 - 09/05/15 06:55 PM (8 years, 4 months ago)

Quote:

It comes off easily when I swipe it with a q-tip like if it was bruising, so I don't know.



Bruising almost never wipes off onto a q-tip. Sporulated mold always will.

Quote:

I thought fully colonized sub was fairly contam resistant, so I'm not sure how this would happen after a full flush. 



Only if the substrate was made with clean spawn. Work on improving your sterile technique when it comes to spawn production, it's possible you're making some mistakes at some point.

Also, did you properly pasteurize your substrate? Coir can be sterilized, but horse poo must be pasteurized correctly.


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OfflineSchism462
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Registered: 07/08/15
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Re: iS THIS TRICH? [Re: TheEaglesGift]
    #22212762 - 09/09/15 03:21 PM (8 years, 4 months ago)

Yes, I pasteurized the coir and hpoo per RR's video.  I also tried every precaution to inoculate using sterile precautions inside a SAB.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: iS THIS TRICH? [Re: Schism462]
    #22214032 - 09/09/15 07:57 PM (8 years, 4 months ago)

What did you inoculate, and with what?

Did you go spores to grain, or spores>agar>grain?


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OfflineKizzle
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Registered: 08/30/11
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Re: iS THIS TRICH? [Re: TheEaglesGift]
    #22216342 - 09/10/15 10:48 AM (8 years, 4 months ago)

Quote:

There has been no signs of contamination on my 100% colonized substrate of hpoo/coir/verm.



I don't know if you saw it before the green or not but that cracking of the substrate can be a sign. Although the mold doesn't exactly crack the substrate itself it makes it crack more easily, particularly if you try to lift it. If the area around a crack like that is unusually squishy it's often because there is mold in it.


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OfflineSchism462
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Re: iS THIS TRICH? [Re: Kizzle]
    #22216965 - 09/10/15 01:42 PM (8 years, 4 months ago)

MS syringe of B+ to rye.  It was lifted  it out of the tub to harvest since all the fruits fruits were on the side.  This is when it cracked, but I assumed it cracked from being too dry.  There wasn't any green or discoloration inside the crack when it happened, but that is where it started.  It's just weird that it started well after I picked the first yield.


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OfflineKizzle
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Re: iS THIS TRICH? [Re: Schism462]
    #22219976 - 09/11/15 03:26 AM (8 years, 4 months ago)

Mechanical damage can trigger sporulation in Trichoderma. Did it turn green about 2 days after it cracked?


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OfflineSchism462
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Registered: 07/08/15
Posts: 23
Last seen: 8 years, 3 months
Re: iS THIS TRICH? [Re: Kizzle]
    #22224928 - 09/11/15 11:09 PM (8 years, 4 months ago)

Yes, it was 2 days after.  It looked like bruising at first but quickly turned green.  That sounds more likely.  Also, the moisture/humidity was never dialed in properly so I think the myc dried out by the time the handful of fruits were harvested from the edges. 

Thanks for the input.  Hopefully the next one goes better.


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