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OfflineCosmic Neuron
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Registered: 08/26/15
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Last seen: 8 years, 3 months
Airborne Contaminants
    #22191478 - 09/05/15 05:54 AM (8 years, 4 months ago)

Greetings, mycologists, cultivators and others.

I wanted to know how to go about getting rid of airborne contaminants for when the fruiting begins.

Should any of you know how, please share your knowledge.


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OfflineCosmic Neuron
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Posts: 50
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Re: Airborne Contaminants [Re: Cosmic Neuron]
    #22191518 - 09/05/15 06:19 AM (8 years, 4 months ago)

Oh, and by the way, I was thinking of using an oil lamp or just a candle to sterilize the air.


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InvisibleMad Season
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Registered: 09/16/12
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Re: Airborne Contaminants [Re: Cosmic Neuron]
    #22191651 - 09/05/15 07:43 AM (8 years, 4 months ago)

Quote:

Cosmic Neuron said:
Greetings, mycologists, cultivators and others.

I wanted to know how to go about getting rid of airborne contaminants for when the fruiting begins.

Should any of you know how, please share your knowledge.



You don't. You rely on your sterile procedures up until spawning. If your grains are clean, everything else should follow suit. Fruiting is far from clean. It's done in open air. I'd be willing to bet my substrates right now are covered in billions of mold spores. Mycelium just has a natural defense against contams. They won't germinate on a fully colonized substrate for a few flushes.

Quote:

Cosmic Neuron said:
Oh, and by the way, I was thinking of using an oil lamp or just a candle to sterilize the air.



Also useless. The only place you gotta be sterile in is your still air box.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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Edited by Mad Season (09/05/15 07:45 AM)


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OfflineCosmic Neuron
Neuron


Registered: 08/26/15
Posts: 50
Loc: The Cosmic Brain
Last seen: 8 years, 3 months
Re: Airborne Contaminants [Re: Mad Season]
    #22191673 - 09/05/15 07:54 AM (8 years, 4 months ago)

Thank you very much for sharing your knowledge, it's really appreciated and I understand what you're saying. Keeping the still air box sterile, shouldn't be too difficult, right?


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InvisibleMad Season
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Re: Airborne Contaminants [Re: Cosmic Neuron]
    #22191682 - 09/05/15 07:59 AM (8 years, 4 months ago)

Not at all, if you have the correct techniques. Check out the improve your sterile techniques link in my signature :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineCosmic Neuron
Neuron


Registered: 08/26/15
Posts: 50
Loc: The Cosmic Brain
Last seen: 8 years, 3 months
Re: Airborne Contaminants [Re: Mad Season]
    #22192082 - 09/05/15 10:23 AM (8 years, 4 months ago)

I read it and I appreciate the effort you put into it. I was also thinking of keeping the tub sterile by cleaning it with alcohol and having a couple oil lamps near by but I'll try both methods and see which works best.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Airborne Contaminants [Re: Cosmic Neuron]
    #22194022 - 09/05/15 06:50 PM (8 years, 4 months ago)

A still air box doesn't work by being sterile, it works because it has still air.

Wipe the box down with soapy water, make sure the top isn't dripping, load it up, let it sit for 20 minutes, go to work quickly but carefully.

You can never get a still air box sterile, don't even bother with that. The wet sides will help trap contaminants in the air, and if the air is still most will have fallen to the bottom of the box and be trapped by the moisture.

Some don't even wet their boxes and still have great success. Still air is key, don't forget that. Unless you're in a positive pressure lab, you will never get your air "sterile".

Candles/lamp do jack shit for the air, use them for flame sterilizing your metal tools though. :super:


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OfflineCosmic Neuron
Neuron


Registered: 08/26/15
Posts: 50
Loc: The Cosmic Brain
Last seen: 8 years, 3 months
Re: Airborne Contaminants [Re: TheEaglesGift]
    #22195633 - 09/06/15 03:13 AM (8 years, 4 months ago)

Hahaha, but I thought that you didn't have to worry too much about colonised cakes getting contaminated.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Airborne Contaminants [Re: Cosmic Neuron]
    #22197913 - 09/06/15 03:53 PM (8 years, 4 months ago)

I think you're confused.

A still air box is used for sterile work like agar and inoculating spawn/cakes.

A fruiting chamber, like a shotgun fruiting chamber, is used to fruit your fully colonized cakes.

They're very different in design, and serve very different functions.


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