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chutney
slappin' the whiners
Registered: 07/26/13
Posts: 402
Last seen: 11 months, 9 days
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Re: Incubator Question [Re: Inocuole]
#22190239 - 09/04/15 09:03 PM (8 years, 4 months ago) |
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Quote:
Inocuole said: I live in the south and it's 110F outside some days. I keep it about 73 in here and that means I never need to adjust anything ever, temp wise.
Ah okay.
So fruiting process won't be affected by low 70s temps? If that's the case I'm golden, I'll just keep the room at 73F. But like I said everything I had read online was leaning more toward 75-80F for fruiting.
Also do you check the inside temp in the fruiting chamber? Does that stay pretty near 73F if that's where your thermostat in the house is set?
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Edited by chutney (09/04/15 09:05 PM)
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Re: Incubator Question [Re: chutney]
#22190347 - 09/04/15 09:24 PM (8 years, 4 months ago) |
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Room temperature is fine for colonization and Fruiting... Don't leave them in the dark... Indirect light from the start works great. 12hrs on 12 hrs off
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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chutney
slappin' the whiners
Registered: 07/26/13
Posts: 402
Last seen: 11 months, 9 days
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Re: Incubator Question [Re: Darkhome]
#22190695 - 09/04/15 10:26 PM (8 years, 4 months ago) |
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Quote:
Darkhome said: Room temperature is fine for colonization and Fruiting... Don't leave them in the dark... Indirect light from the start works great. 12hrs on 12 hrs off
Cool. Room temp it is. Are you saying indirect light through colonization as well??
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Incubator Question [Re: chutney]
#22190701 - 09/04/15 10:28 PM (8 years, 4 months ago) |
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Yes. Indirect lighting is always at least a little good. Certainly no harm in it.
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chutney
slappin' the whiners
Registered: 07/26/13
Posts: 402
Last seen: 11 months, 9 days
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Re: Incubator Question [Re: Inocuole]
#22191552 - 09/05/15 06:44 AM (8 years, 4 months ago) |
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Cool.
So I left the AC on max in that room all night. Just checked temp inside the tub and it was about 77. So it hardly fucked with the temp in the incubator at all. I should be good I think
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xpsprogamer
TrippyTrauma

Registered: 07/17/15
Posts: 98
Loc: NorthEast U.S.
Last seen: 3 years, 3 months
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Re: Incubator Question [Re: chutney]
#22192116 - 09/05/15 10:38 AM (8 years, 4 months ago) |
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Quote:
chutney said:
Quote:
xpsprogamer said: the incubator doesnt need air flow just needs to be warm and dark... if its all inoculated and in the incubator its time to forget it for about 5 days... just let the magic happen. . incubating is the easiest part. it really comes down to your sterile tek. if that is on point u will be all set.
Right on. Thank you brother!
Everything is all inoculated and I have 6 three pound rye bags (rye berries, a mix of distilled & vinegar water, gypsum and diammonium phosphate and a couple other ingredients) sitting in the tub. People have had almost universal success with these bags, with whatever failures being attributed to the stupidity of the reviewer and their inability to research properly or follow direction so I'm stoked!
It should be just about as sterile as can possibly be. I don't believe we can talk vendors here but the rye spawn bags were purchased from one of the most reliable vendors on the net. They come pre-made under sterile conditions and are also pressure sterilized at 15psi for 20 minutes. There are small patches at the top of each bag that allow gas exchange but are made of a filter material that is too fine to let most contaminants in. I cleaned the room I inoculated in from top to bottom yesterday. Then today I wiped everything down with isopropyl alcohol including the containers, the self-healing injection ports and the syringe tips. After I wiped down the tips of the syringes I then also heated them for about 3-5 seconds with a lighter until red hot then waited about 5 seconds and proceeded to inoculate each bag like this.
So I should be good and sterile. If not then I don't know what else I could have done.
Another quick question. I don't plan on opening the tub for at least 5-7 days to check for signs of colonization. After colonization gets going and is almost done, do I have to be very careful about opening the tub and exposing it to light? Is it possibly for only a few seconds/minutes of light to trigger accidental fruiting? I would think not but rather safe than sorry.
First things first a SAB (Still Air Box) is really easy to build and it would do u wonders in being sterile. i made a GB (Glove Box) but it isnt necessary to build one SAB's work just as well if not better cuz getting the glove off ur hands is annoying.
my incubator was a 3 foot by 4 foot closet what we put a space heater in on the lowest setting. we had a thermometer in the room track the temp. my jars went in a small white tub and i put the tub in a trash bag. left it in the middle of the closet on a table. then closed the door. after the first 4 days i had to shake my jars then it was every 3 days. the incubator u have is really good for colder climates in my opinion.
As for opening the tub and exposing the bags to light. yes it is important for them to be in a dark and warm area. it is fine to pull them out and expose to a bit of light... that doesnt mean put them in direct light, it means that a room with blinds/curtains close will not hurt them. when out of the tub i would try to diffently keep ur proceedings to no more than 10-15 mins to be on the safe side.
i just got done my first grow and i actually test my first harvest last night. your honestly just overthinking it take a deep breath and let the magic happen. contamination is something that even the best of the best have to deal with. like everything practice makes perfect. ill be instered in watching you project.
take a look at mine HERE.
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Green Bastard
Stranger

Registered: 02/26/14
Posts: 199
Loc: Canada
Last seen: 3 years, 8 months
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^^^^^
You just finished your first grow, and you're giving people BS advice on light exposure??
Slow down kid....
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chutney
slappin' the whiners
Registered: 07/26/13
Posts: 402
Last seen: 11 months, 9 days
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Quote:
xpsprogamer said:
Quote:
chutney said:
Quote:
xpsprogamer said: the incubator doesnt need air flow just needs to be warm and dark... if its all inoculated and in the incubator its time to forget it for about 5 days... just let the magic happen. . incubating is the easiest part. it really comes down to your sterile tek. if that is on point u will be all set.
Right on. Thank you brother!
Everything is all inoculated and I have 6 three pound rye bags (rye berries, a mix of distilled & vinegar water, gypsum and diammonium phosphate and a couple other ingredients) sitting in the tub. People have had almost universal success with these bags, with whatever failures being attributed to the stupidity of the reviewer and their inability to research properly or follow direction so I'm stoked!
It should be just about as sterile as can possibly be. I don't believe we can talk vendors here but the rye spawn bags were purchased from one of the most reliable vendors on the net. They come pre-made under sterile conditions and are also pressure sterilized at 15psi for 20 minutes. There are small patches at the top of each bag that allow gas exchange but are made of a filter material that is too fine to let most contaminants in. I cleaned the room I inoculated in from top to bottom yesterday. Then today I wiped everything down with isopropyl alcohol including the containers, the self-healing injection ports and the syringe tips. After I wiped down the tips of the syringes I then also heated them for about 3-5 seconds with a lighter until red hot then waited about 5 seconds and proceeded to inoculate each bag like this.
So I should be good and sterile. If not then I don't know what else I could have done.
Another quick question. I don't plan on opening the tub for at least 5-7 days to check for signs of colonization. After colonization gets going and is almost done, do I have to be very careful about opening the tub and exposing it to light? Is it possibly for only a few seconds/minutes of light to trigger accidental fruiting? I would think not but rather safe than sorry.
First things first a SAB (Still Air Box) is really easy to build and it would do u wonders in being sterile. i made a GB (Glove Box) but it isnt necessary to build one SAB's work just as well if not better cuz getting the glove off ur hands is annoying.
my incubator was a 3 foot by 4 foot closet what we put a space heater in on the lowest setting. we had a thermometer in the room track the temp. my jars went in a small white tub and i put the tub in a trash bag. left it in the middle of the closet on a table. then closed the door. after the first 4 days i had to shake my jars then it was every 3 days. the incubator u have is really good for colder climates in my opinion.
As for opening the tub and exposing the bags to light. yes it is important for them to be in a dark and warm area. it is fine to pull them out and expose to a bit of light... that doesnt mean put them in direct light, it means that a room with blinds/curtains close will not hurt them. when out of the tub i would try to diffently keep ur proceedings to no more than 10-15 mins to be on the safe side.
i just got done my first grow and i actually test my first harvest last night. your honestly just overthinking it take a deep breath and let the magic happen. contamination is something that even the best of the best have to deal with. like everything practice makes perfect. ill be instered in watching you project.
take a look at mine HERE.
Hey thanks for the pointers my friend!
It's easy to overthink things and second guess your process and procedures and equipment because there's a staggering amount of tek's online and it's hard to get consensus yanno? Plus every time I go to a forum or website for help somebody will give me a piece of advice or pointers that contradict the advice given to me by someone else. However given that all these people claim success with such varying methods, I like to think that this points to a certain hardiness or robustness of psilocybe once you get past the critical factors like sterilization and temperature and whatnot. It seems it wants to thrive and will tolerate a certain amount of tinkering fairly well provided your not totally fucking it up with light, contaminants or wild temperature fluctuations.
I've checked the temp in the incubator a few times and its pretty consistently 75-79F so things should be good on the temperature front. At least for colonization anyways. Inoculated on 9/4 so I'm hoping by around 9/11-9/15 I'll see good colonization happening. Unfortunately I do have to leave town for three days leaving 9/13 and returning home on 9/17. I will be out of state during this time so I'm really hoping nothing gets fucked up with my grow. I considered waiting to inoculate until after I returned home but I was very unsure and had heard conflicting information on how sensitive psilocybe is with temperature variation and that would push the fruiting process into late October/early November. I figured it wiser to try it now, especially considering it's my first grow, when we still have warm weather in NY... the whole month of September is usually 70s and 80s. So I went ahead and set everything up and inoculated and whatnot and after checking the temp in various situations (at night, with the AC on, with AC off etc) now I'm confident my incubator will be fine for use during the winter months and I was just being paranoid. But hopefully things will be fine for those three days when I'm unable to check on them. At that point they will still be less than 2 full weeks into colonization and aside from an occasional peek, colonization is pretty much "set and forget" for extended periods of time. So I think I should be fine. My only concern would be if I have a contamination issue that requires immediate removal or other immediate action and wouldn't be home to address it in time. Hopefully that doesn't occur.
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Edited by chutney (09/06/15 08:52 AM)
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Incubator Question [Re: chutney]
#22196224 - 09/06/15 08:59 AM (8 years, 4 months ago) |
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iSmkGrnBud and Inocuole are right on. OP and bart, quit wasting all of our natural resources so your fat asses can be cool. Go fucking exercise and you'll enjoy 70
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Incubator Question [Re: Inocuole]
#22196245 - 09/06/15 09:04 AM (8 years, 4 months ago) |
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iSmkGrnBud said: I'd hate to see all your guy's electric bills. Killer.
Quote:
iSmkGrnBud said:
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chutney said: Nah it ain't bad.
Shit is super efficient these days. I'm just running two window AC units and my electric bill in the summer isn't much higher. Between $20-40 extra usually (per month), depending on how much I run them. One of them isn't used often but the other runs probably close to 8-12 hours a day. Also note these are small like 5,000 BTU units designed to cool a small-medium sized room.
I am a little worried how this is going to effect the ability of the aquarium heater to keep the inside of the incubator at 75-80 when the ambient room temp is like 65 and even colder at night. I'm going to leave AC on all night and check the thermometer in the incubator in the morning. If need be I may have to get a second aquarium heater or something. But I'm hoping I'll be alright. Cause I live in NY and it's hot and humid as fuck and has been like high 80s with like 100% humidity the past few days so I definitely need my AC lol.
You cant tell me window units arent power hungry. They are. But with that said its still your house and your choice. Just seems cold as hell for comfortable temps. I'm down here in the bible belt. Constantly 90+ and probably more humid than the Amazon (lol), but I still keep my house between 72-74 and am never uncomfortable. You're used to that NY weather though, screw all that.
Trust me though, if youre gonna do anything more than those jars just up the temperature in your place and ditch the incubator. You're still gonna want temps in the 70s for fruiting so that incubator will be useless at that stage. If you ever work with agar, your gonna have to incubate those, which would be a pain. Opinions though, not everything works for everyone.
Quote:
Inocuole said: It's just weird that you're using even more electricity to combat the electricity you're already using to make it cool.
Quote:
Inocuole said: Yeah you can't really incubate a fruiting chamber and have it work out. Would be better off learning to enjoy 70F.
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 10 days, 23 hours
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Re: Incubator Question [Re: azur]
#22196406 - 09/06/15 09:48 AM (8 years, 4 months ago) |
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Quote:
azur said: iSmkGrnBud and Inocuole are right on. OP and bart, quit wasting all of our natural resources so your fat asses can be cool. Go fucking exercise and you'll enjoy 70

Even though OP is ignoring me
Edited by iSmkGrnBud (09/06/15 12:16 PM)
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: Incubator Question [Re: azur]
#22197024 - 09/06/15 12:26 PM (8 years, 4 months ago) |
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Quote:
azur said: iSmkGrnBud and Inocuole are right on. OP and bart, quit wasting all of our natural resources so your fat asses can be cool. Go fucking exercise and you'll enjoy 70
ima a fit man, ill come put your bitch ass in my incubator
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Why don't you put your girl in there and warm her up for me. Not too warm though, cause we don't want the bacteria to soar.
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: Incubator Question [Re: azur]
#22197105 - 09/06/15 12:48 PM (8 years, 4 months ago) |
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Quote:
azur said: Why don't you put your girl in there and warm her up for me. Not too warm though, cause we don't want the bacteria to soar.
Quote:
azur said: Why don't you put your girl in there and warm her up for me. Not too warm though, cause we don't want the bacteria to soar.
Gives me a great idea to swap her vagina and grow it out
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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I can send a swab of my urethra on a plate if you like?
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 10 days, 23 hours
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Re: Incubator Question [Re: azur]
#22197166 - 09/06/15 01:03 PM (8 years, 4 months ago) |
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-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: Incubator Question [Re: azur]
#22197182 - 09/06/15 01:07 PM (8 years, 4 months ago) |
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Quote:
azur said: I can send a swab of my urethra on a plate if you like?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
iSmk Grn Bud said:
Quote:
a z u r said: iSmkGrnBud and Inocuole are right on. OP and bart, quit wasting all of our natural resources so your fat asses can be cool. Go fucking exercise and you'll enjoy 70

Even though OP is ignoring me 
OP stop ignoring this dude, he's giving you good advice and ignoring people is for pussies who can't handle real life.
Edited by Inocuole (09/06/15 05:51 PM)
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Incubator Question [Re: Inocuole]
#22198135 - 09/06/15 04:30 PM (8 years, 4 months ago) |
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He's ignoring me too
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Incubator Question [Re: Inocuole]
#22198449 - 09/06/15 05:52 PM (8 years, 4 months ago) |
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Quote:
Inocuole said:
Quote:
iSmk Grn Bud said:
Quote:
a z u r said: iSmkGrnBud and Inocuole are right on. OP and bart, quit wasting all of our natural resources so your fat asses can be cool. Go fucking exercise and you'll enjoy 70

Even though OP is ignoring me 
OP stop ignoring this dude, he's giving you good advice and ignoring people is for pussies who can't handle real life.
There, he can see this one then. Ignoring these two dudes is not beneficial to anyone who wants to learn.
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