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Offlinechutney
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Registered: 07/26/13
Posts: 402
Last seen: 11 months, 9 days
Incubator Question
    #22189102 - 09/04/15 05:25 PM (8 years, 4 months ago)

Hi all,

I have a question regarding cubensis in a Tub-in-Tub incubator with aquarium heater.

Does the incubator need a lot of airflow? Currently I have two of the same size plastic bins. One is filled about 1/3rd of the way up with water. The other bin is cut almost in half and sits on the water. I am using pre-purchased Rye spawn bags that have self-healing injection ports which auto-close when pull the syringe out. The bags are closed but have patches at the top which allow for gas exchange.

Does there need to be any sort of airflow inside the incubator tub? Right now the bags are in there and the lid on and I just inoculated today. Is whatever airflow that sneaks in under the lid (these things aren't totally airtight) sufficient? I'd rather not fuck around with them now that I've inoculated them and are letting them colonize. But if the airflow thing is going to fuck it up then obviously I gotta take some preventive action here.

What y'all think?

Thanks in advance. Love this place, love you people


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Offlinexpsprogamer
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Re: Incubator Question [Re: chutney]
    #22189122 - 09/04/15 05:30 PM (8 years, 4 months ago)

the incubator doesnt need air flow just needs to be warm and dark... if its all inoculated and in the incubator its time to forget it for about 5 days... just let the magic happen. . incubating is the easiest part. it really comes down to your sterile tek. if that is on point u will be all set.


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: Incubator Question [Re: chutney]
    #22189127 - 09/04/15 05:30 PM (8 years, 4 months ago)

Quote:

chutney said:
Hi all,

I have a question regarding cubensis in a Tub-in-Tub incubator with aquarium heater.

Does the incubator need a lot of airflow? Currently I have two of the same size plastic bins. One is filled about 1/3rd of the way up with water. The other bin is cut almost in half and sits on the water. I am using pre-purchased Rye spawn bags that have self-healing injection ports which auto-close when pull the syringe out. The bags are closed but have patches at the top which allow for gas exchange.

Does there need to be any sort of airflow inside the incubator tub? Right now the bags are in there and the lid on and I just inoculated today. Is whatever airflow that sneaks in under the lid (these things aren't totally airtight) sufficient? I'd rather not fuck around with them now that I've inoculated them and are letting them colonize. But if the airflow thing is going to fuck it up then obviously I gotta take some preventive action here.

What y'all think?

Thanks in advance. Love this place, love you people



frank has a good incubator tek here is mine there is enough air in the box for the jars but i only use mine during the cold months


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OfflineFungitarianism
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Re: Incubator Question [Re: chutney]
    #22189146 - 09/04/15 05:35 PM (8 years, 4 months ago)

I thought incubators where a thing of the past honestly.


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: Incubator Question [Re: Fungitarianism]
    #22189171 - 09/04/15 05:40 PM (8 years, 4 months ago)

Quote:

Fungitarianism said:
I thought incubators where a thing of the past honestly.



how ? Jars take almost 3 times as long in the cold months for me if i dont keep them normal temps tell me why this is a thing of the past.


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OfflineFungitarianism
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Re: Incubator Question [Re: mrbart4444]
    #22189216 - 09/04/15 05:49 PM (8 years, 4 months ago)

Well, I have read a lot of people here say to stay away from them. But, I think that is said for n00bs anyway.
I hear that mycelium likes temps that humans find to be comfortable. You must live in a cold ass house I guess.


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Offlinechutney
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Re: Incubator Question [Re: xpsprogamer]
    #22189300 - 09/04/15 06:03 PM (8 years, 4 months ago)

Quote:

xpsprogamer said:
the incubator doesnt need air flow just needs to be warm and dark... if its all inoculated and in the incubator its time to forget it for about 5 days... just let the magic happen. . incubating is the easiest part. it really comes down to your sterile tek. if that is on point u will be all set.





Right on. Thank you brother!

Everything is all inoculated and I have 6 three pound rye bags (rye berries, a mix of distilled & vinegar water, gypsum and diammonium phosphate and a couple other ingredients) sitting in the tub. People have had almost universal success with these bags, with whatever failures being attributed to the stupidity of the reviewer and their inability to research properly or follow direction so I'm stoked!

It should be just about as sterile as can possibly be. I don't believe we can talk vendors here but the rye spawn bags were purchased from one of the most reliable vendors on the net. They come pre-made under sterile conditions and are also pressure sterilized at 15psi for 20 minutes. There are small patches at the top of each bag that allow gas exchange but are made of a filter material that is too fine to let most contaminants in. I cleaned the room I inoculated in from top to bottom yesterday. Then today I wiped everything down with isopropyl alcohol including the containers, the self-healing injection ports and the syringe tips. After I wiped down the tips of the syringes I then also heated them for about 3-5 seconds with a lighter until red hot then waited about 5 seconds and proceeded to inoculate each bag like this.

So I should be good and sterile. If not then I don't know what else I could have done. 

Another quick question. I don't plan on opening the tub for at least 5-7 days to check for signs of colonization. After colonization gets going and is almost done, do I have to be very careful about opening the tub and exposing it to light? Is it possibly for only a few seconds/minutes of light to trigger accidental fruiting? I would think not but rather safe than sorry.


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Edited by chutney (09/04/15 06:08 PM)


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Invisiblemrbart4444
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Registered: 09/13/14
Posts: 2,266
Re: Incubator Question [Re: mrbart4444]
    #22189344 - 09/04/15 06:10 PM (8 years, 4 months ago)

Quote:

mrbart4444 said:
Quote:

Fungitarianism said:
I thought incubators where a thing of the past honestly.



how ? Jars take almost 3 times as long in the cold months for me if i dont keep them normal temps tell me why this is a thing of the past.




my house stays at around 60-65 in the winter



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Offlinechutney
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Re: Incubator Question [Re: Fungitarianism]
    #22189350 - 09/04/15 06:12 PM (8 years, 4 months ago)

Quote:

Fungitarianism said:
Well, I have read a lot of people here say to stay away from them. But, I think that is said for n00bs anyway.
I hear that mycelium likes temps that humans find to be comfortable. You must live in a cold ass house I guess.




I use AC all summer. Its like 65 in my house all the time. At night I keep it at like 60F as I like to sleep in a nice cool room.

I doubt you'd have much luck with those temps. Even if you did I'm sure the colonization would take forever.


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OfflineiSmkGrnBud
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Re: Incubator Question [Re: chutney]
    #22189456 - 09/04/15 06:36 PM (8 years, 4 months ago)

I'd hate to see all your guy's electric bills. Killer.


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Offlinechutney
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Re: Incubator Question [Re: iSmkGrnBud]
    #22189557 - 09/04/15 06:57 PM (8 years, 4 months ago)

Nah it ain't bad.

Shit is super efficient these days. I'm just running two window AC units and my electric bill in the summer isn't much higher. Between $20-40 extra usually (per month), depending on how much I run them. One of them isn't used often but the other runs probably close to 8-12 hours a day. Also note these are small like 5,000 BTU units designed to cool a small-medium sized room.

I am a little worried how this is going to effect the ability of the aquarium heater to keep the inside of the incubator at 75-80 when the ambient room temp is like 65 and even colder at night. I'm going to leave AC on all night and check the thermometer in the incubator in the morning. If need be I may have to get a second aquarium heater or something. But I'm hoping I'll be alright. Cause I live in NY and it's hot and humid as fuck and has been like high 80s with like 100% humidity the past few days so I definitely need my AC lol.


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Edited by chutney (09/04/15 06:57 PM)


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OfflineiSmkGrnBud
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Re: Incubator Question [Re: chutney]
    #22189643 - 09/04/15 07:10 PM (8 years, 4 months ago)

Quote:

chutney said:
Nah it ain't bad.

Shit is super efficient these days. I'm just running two window AC units and my electric bill in the summer isn't much higher. Between $20-40 extra usually (per month), depending on how much I run them. One of them isn't used often but the other runs probably close to 8-12 hours a day. Also note these are small like 5,000 BTU units designed to cool a small-medium sized room.

I am a little worried how this is going to effect the ability of the aquarium heater to keep the inside of the incubator at 75-80 when the ambient room temp is like 65 and even colder at night. I'm going to leave AC on all night and check the thermometer in the incubator in the morning. If need be I may have to get a second aquarium heater or something. But I'm hoping I'll be alright. Cause I live in NY and it's hot and humid as fuck and has been like high 80s with like 100% humidity the past few days so I definitely need my AC lol.




You cant tell me window units arent power hungry. They are. But with that said its still your house and your choice. Just seems cold as hell for comfortable temps. I'm down here in the bible belt. Constantly 90+ and probably more humid than the Amazon (lol), but I still keep my house between 72-74 and am never uncomfortable. You're used to that NY weather though, screw all that.

Trust me though, if youre gonna do anything more than those jars just up the temperature in your place and ditch the incubator. You're still gonna want temps in the 70s for fruiting so that incubator will be useless at that stage. If you ever work with agar, your gonna have to incubate those, which would be a pain. Opinions though, not everything works for everyone.


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Offlinechutney
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Re: Incubator Question [Re: iSmkGrnBud]
    #22189979 - 09/04/15 08:11 PM (8 years, 4 months ago)

Well I'm just saying what I pay on my electric bills, its only $20-40 extra per month. That's nothing financially so fuck it. I'm completely fine with paying like $100-130ish extra on my bills throughout the season for complete comfort indoors for 3-4 months. Not everyone has the same preferences for room temperature and given that I have a certain propensity to sweat like a mofo I like to keep it pretty cool.

Anyways why do you say to ditch the incubator for fruiting? That is going to be almost a month down the line and in NY it can get chilly in October. This apartment is also a tad drafty and probably not the best insulated and could use an upgrade to some more energy efficient windows so the incubator really gives peace of mind... say for instance everyone is at work and I accidentally left a window open in the house.

How bad does psilocybe cold shock? With cannabis the first hard frost can fuck up your crop. Like temps below freezing for just a matter of hours. So given that there's a precise range of temps you want to stay within for fruiting, would it negatively effect the fungi if I left a window open on a 55-60F fall day and went to work for 12 hours? Or would you have to leave it in those temps for much longer for it to produce a really negative effect on your fungi? Not sure how hardy it is... So all this, coupled with the fact that I am new to this, is why I opt for the incubator. Maybe it's just peace of mind and not much more, but given I have time and money invested in this I'd rather roll with the safeguard of an incubator for now.


--------------------


Edited by chutney (09/04/15 08:28 PM)


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InvisibleInocuole
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Re: Incubator Question [Re: chutney]
    #22190018 - 09/04/15 08:20 PM (8 years, 4 months ago)

It's just weird that you're using even more electricity to combat the electricity you're already using to make it cool.


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OfflineiSmkGrnBud
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Re: Incubator Question [Re: chutney]
    #22190033 - 09/04/15 08:23 PM (8 years, 4 months ago)

Quote:

chutney said:
Well I'm just saying what I pay on my electric bills, its only $20-40 extra per month. That's nothing financially so fuck it. I'm completely fine with paying like $100-130ish extra on my bills throughout the season for complete comfort indoors for 3-4 months. Not everyone has the same preferences for room temperature and given that I have a certain propensity to sweat like a mofo I like to keep it pretty cool.




Well lets not make this about your preferences. Of course we all want things to be perfect and suitable to our liking's. And it is your life and your house, nothing you say will be wrong. But you're trying to cultivate mycelium, you have to think about its preferences as well. I mean an incubator was a thought, but its gonna be an even harder process down the road to keep that myc happy and healthy.


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Re: Incubator Question [Re: iSmkGrnBud]
    #22190069 - 09/04/15 08:28 PM (8 years, 4 months ago)

Yeah you can't really incubate a fruiting chamber and have it work out.  Would be better off learning to enjoy 70F.


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OfflineiSmkGrnBud
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Re: Incubator Question [Re: Inocuole]
    #22190078 - 09/04/15 08:30 PM (8 years, 4 months ago)

:whathesaid:


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Offlinechutney
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Re: Incubator Question [Re: Inocuole]
    #22190129 - 09/04/15 08:40 PM (8 years, 4 months ago)

Quote:

Inocuole said:
Yeah you can't really incubate a fruiting chamber and have it work out.




Yeah by the time fruiting rolls around it will be October most likely and I will just use a programmable heater to keep the ambient room temp where it needs to be.

Will be hot as fuck in this place during those few weeks of fruiting most likely....will prolly be 60s or upper 50s outside still in NY at that point and I'll have the thermostat set to mid 70s indoors lol.

How do you handle fruiting man? Do you live in a climate where temp is always in the sweet spot and thus just leave ambient room temp and not run AC's or nothing? We're heading into the falls months so it's going to be a little tricky up north.

If the ambient room temp is 70F how can I go about beginning the fruiting process (in the most efficient way possible) without introducing heaters or equipment setups to control temps?  Most things I have read say you wanna be between 75-78F.. Especially at night in NY in October... gets down to 40s and 50s outside.


Edited by chutney (09/04/15 08:49 PM)


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OfflineiSmkGrnBud
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Re: Incubator Question [Re: chutney]
    #22190161 - 09/04/15 08:46 PM (8 years, 4 months ago)

Quote:

chutney said:
Quote:

Inocuole said:
Yeah you can't really incubate a fruiting chamber and have it work out.




Yeah by the time fruiting rolls around it will be October most likely and I will just use a programmable heater to keep the temp where it needs to be.

Will be hot as fuck those few weeks most likely....will prolly be 60s or upper 50s still in NY at that point- not really weather that would require you to have the heat set at 75 indoors lol.

How do you handle fruiting man? Do you live in a climate where temp is always in the sweet spot and thus just leave ambient room temp and not run AC's or nothing? We're heading into the falls months so it's going to be a little tricky up north.




Then dont set it for 75. 70 would be fine. With 50-60 degree weather outside, that should be close to where ambient temperature will rest without heat or AC inside a home. And even if its not, it wont take much at all. Like I said before I just keep my thermostat between 72 and 74. I have central air though, not window units. It can get in the negatives here in winter (very rare) and gets up to the 100's in summers where I live. Trust me, its not hard to control temperature in a home. Kind of what they were designed for.


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Re: Incubator Question [Re: iSmkGrnBud]
    #22190225 - 09/04/15 09:01 PM (8 years, 4 months ago)

I live in the south and it's 110F outside some days.  I keep it about 73 in here and that means I never need to adjust anything ever, temp wise.


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