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forrest



Registered: 11/16/12
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C. militaris endeavours 1
#22185898 - 09/03/15 11:40 PM (8 years, 4 months ago) |
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In the past i had a CS4 culture, but it the myc smelled very strong of ammonia. Now i have this militaris culture, it doesn't smell foul at all, it turns orange/red a bit on the bottom, and seems to be pinning (on substrate of rye and rice) but not fruiting yet. Next to that i received another militaris culture that's originally from a South Korean company (don;t know which), so i'll be making jars of that one too this month.
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Stropharis



Registered: 04/02/07
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Re: C. militaris endeavours [Re: forrest]
#22187158 - 09/04/15 10:01 AM (8 years, 4 months ago) |
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Awesome!
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foragedfungus


Registered: 09/30/13
Posts: 1,849
Loc: out there
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Re: C. militaris endeavours [Re: Stropharis] 1
#22189605 - 09/04/15 07:06 PM (8 years, 4 months ago) |
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That is awesome! Please keep us updated. I am very interested to see your progress/results with this species, and would love any more details you can share.
I have been finding lots of C. militaris this year. I would like to try cultivating it. After trying to clone four different fruits, I think I've finally got a clean culture going.
Here is one I found today. Going to take clones from this one too.
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RogerSmith

Registered: 01/29/15 
Posts: 365
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I wish I could find wild cordyceps too! They look like militaris. Have you found any other cordyceps species? There are many undiscovered and exotic-looking cordyceps species. What is the best time to look for wild cordyceps? What was the temperature of air when you found most of them? Around 20 degrees / 68 F or colder?
Fruiting militaris from fresh culture isn't hard. Just inoculate grains and put them on light once colonized.
Since we r sharing pics, I recently caught a moth, which got sterilized together with some rice. It fully colonized last week. I am wondering if any fruits will grow directly from moth.
 
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
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Loc: The Golden State
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Re: C. militaris endeavours [Re: RogerSmith]
#22192329 - 09/05/15 11:55 AM (8 years, 4 months ago) |
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Cordyceps porn! I really hope your subs fruit, there's nothing more awesome than in vitro cordyceps.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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foragedfungus


Registered: 09/30/13
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Loc: out there
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Re: C. militaris endeavours [Re: RogerSmith] 1
#22192659 - 09/05/15 01:19 PM (8 years, 4 months ago) |
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Quote:
RogerSmith said: I wish I could find wild cordyceps too! They look like militaris. Have you found any other cordyceps species? There are many undiscovered and exotic-looking cordyceps species. What is the best time to look for wild cordyceps? What was the temperature of air when you found most of them? Around 20 degrees / 68 F or colder?
The one pictured is C. militaris. Here is a thread with my cordyceps finds for this year- http://www.shroomery.org/forums/showflat.php/Number/22127308 I found all of them in upstate NY from the end of july until now (yesterday). Temps in the high 70's/low 80's(F) during the day, 60's at night.
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forrest



Registered: 11/16/12
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Really nice to see more people playing around with it I'm also thinking of giving it some insects as food.
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foragedfungus


Registered: 09/30/13
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Re: C. militaris endeavours [Re: forrest]
#22417421 - 10/22/15 09:45 AM (8 years, 3 months ago) |
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Hey Forrest, any updates for us?? I'd really like to know how this project is working out for you.
After many bacterial contam issues, I think I finally have my wild clone all cleaned up and ready for grains.
(damn it's hard to take a good picture of a no-pour dish!)
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forrest



Registered: 11/16/12
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Last seen: 4 years, 6 months
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jars of the second strain are colonising now, hoping they will do better. The one i posted a picture of stalled...
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RogerSmith

Registered: 01/29/15 
Posts: 365
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Re: C. militaris endeavours [Re: forrest]
#22423654 - 10/23/15 04:23 PM (8 years, 3 months ago) |
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Re: C. militaris endeavours [Re: RogerSmith]
#22424637 - 10/23/15 08:30 PM (8 years, 3 months ago) |
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Was that just rice and water with a moth?
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RogerSmith

Registered: 01/29/15 
Posts: 365
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Re: C. militaris endeavours [Re: MrGiraffe]
#22424772 - 10/23/15 09:00 PM (8 years, 3 months ago) |
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Yes
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forrest



Registered: 11/16/12
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Re: C. militaris endeavours [Re: RogerSmith]
#22425546 - 10/24/15 01:09 AM (8 years, 3 months ago) |
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great
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MrGiraffe

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Re: C. militaris endeavours [Re: RogerSmith]
#22425827 - 10/24/15 04:43 AM (8 years, 3 months ago) |
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Quote:
RogerSmith said: Yes
Where did you get your culture from?
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RogerSmith

Registered: 01/29/15 
Posts: 365
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Re: C. militaris endeavours [Re: MrGiraffe]
#22426029 - 10/24/15 07:03 AM (8 years, 3 months ago) |
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I cloned a fruit
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drake89
Mushroom Magnate



Registered: 06/26/11
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Re: C. militaris endeavours [Re: RogerSmith]
#22426477 - 10/24/15 10:10 AM (8 years, 3 months ago) |
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seems like most people with success fruiting get them from wild clones.
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foragedfungus



Registered: 09/30/13
Posts: 1,849
Loc: out there
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Re: C. militaris endeavours [Re: drake89]
#22642121 - 12/11/15 10:24 AM (8 years, 1 month ago) |
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32 days ago I inoculated 12 small jars of oats with my wild C. militaris clone. 11 were fully colonized at 10-14 days. One had a spot of mold and was discarded. In the past few days most have begun to show a slight orange color.

What is my next step towards fruiting? Do I change the amount/intensity of light? Change the amount of GE in the jar lids?(poly-fil filters) Just keep waiting?
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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"Troopin cordyceps (Cordyceps militaris )is also called north Chinese caterpillar fungus, north awe to in China. It belongs to Corcyceps, Clavicipitales, Pyrenomycetes, Ascomycotina, Troopin cordyceps and awe to that is widely known in China are both Corcyceps, because it's a typical breed in Cordyceps, its widely received by the scholars all over the world. The artificial cultivation of Troopin cordyceps has achieved success and a series of produces has been opened up. This will open a new route for nourishing food and functional food. In pace with development of science and technology, the cultivation and processing of Troopin cordyceps would have brilliant prospects.
As cultivating Troopin cordyceps, firstly inoculating spawn in medium to form a large number of conidiums. Secondly, choosing conidiums to make suspended spore liquid by aseptic water, then inoculate it at host, which means pupa. Protect the pupa until it become stiff. Then put the stiff pupa in simulated natural environment like wild Troopin cordyceps in it and culture it. The seed stand that grows from pupa will be exactly alike as from wild pupa.
The detailed method of operation follows:
Screening strain Rejuvenate Troopin cordyceps through live pupas to screen a spawn which sensitizer is strong and easy to form spores. Making suspended spore liquid Inoculate the strain in medium and culture it at 20°C. When conidiums produce, choose a part of it to make suspended spore liquid by aseptic water. It will be used later. Preparing hosts Disinfect with smoke after the silkworms go up a small bundle of straw to spin cocoons on. Cut open the cocoon and take the pupa a week later. Clean out the bad and sick pupas. Choose healthy pupas in growth phase. Or choose the five-years-old silkworms to be hosts which have not gone up a small bundle of straw to spin cocoons on. Inoculation Sting into the pupa by an inoculation needle, which has dipped in spores suspended liquid. The rate of infection may be upon 95%. Protecting the pupas which have been infected Spread out the pupas which have been infected in a big round shallow basket, protect the pupas to stiff at indoor temperature. Breeding the fruit body Imitate natural environment of Troopin cordyceps. Culture the stiff pupas to form fruit body. The method is covering the stiff pupas with a coat of porous material such as bits of sponge, small piece of coal, etc. Breed the fruit body under circumstances of indoor temperature and 95% air humidity. Directive breeding of fruit body The purpose of directive breeding of fruit body is to make the fruit body long and stiff. The method is stick the upward head of pupa into porous material such as sponge, breed it under the aforementioned conditions. Collecting and preserving the strain of Troopin cordyceps Dig up the fruit body as it has been matured, clean it and disinfect its body with edible alcohol. Then dry it at 60°C. Seal up and store it at a cool, dark, and arid place. Troopin cordyceps cultured in fore mentioned way, it needs 35-45 days from inoculation to mature period of the fruit body. It will harvest 50g-350g per square meter. According to examination, Cordyceps militaris artificial cultured has a great deal of awe to acid, awe to element, amino acid, trace element and alkaloid, etc. It can enrich the marrow, resist cancer, calm and diminish inflammation, stop blooding and reduce phlegm. It has a good curative effect to nephritis, hepatitis, asthma and neurasthenia."
Kinda interesting, says 35-45 days... I'll slowly be doing research myself
Edited by Raven44 (12/11/15 10:38 AM)
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foragedfungus



Registered: 09/30/13
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Loc: out there
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Re: C. militaris endeavours [Re: Raven44]
#22642245 - 12/11/15 11:00 AM (8 years, 1 month ago) |
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In the article you are quoting they are using pupae as substrate. Cutting open the individual cocoons, and injecting each caterpillar with a needle dipped in "spores suspended liquid"!! That sounds like an intensive project for me to undertake.
I've seen it fruited from grains. (usually rice). Not sure what effect it will have on the compounds produced by the fungus, but it seems a little more doable for me.
I know rogersmith has had success with this species before, hopefully he will stop in with some information.
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RogerSmith

Registered: 01/29/15 
Posts: 365
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Can't help ya here. Some grow, some doesn't. Were it that easy, everyone would be growing it.
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
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Loc: My sovereign reality bubble
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Quote:
foragedfungus said: In the article you are quoting they are using pupae as substrate. Cutting open the individual cocoons, and injecting each caterpillar with a needle dipped in "spores suspended liquid"!! That sounds like an intensive project for me to undertake.
I've seen it fruited from grains. (usually rice). Not sure what effect it will have on the compounds produced by the fungus, but it seems a little more doable for me.
I know rogersmith has had success with this species before, hopefully he will stop in with some information.
I know the quote was not very relevant, I will come w more pertinent info soon. It was interesting is all
Edited by Raven44 (12/11/15 01:31 PM)
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Raven44
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Registered: 12/07/13
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Quote:
foragedfungus said: 32 days ago I inoculated 12 small jars of oats with my wild C. militaris clone. 11 were fully colonized at 10-14 days. One had a spot of mold and was discarded. In the past few days most have begun to show a slight orange color.

What is my next step towards fruiting? Do I change the amount/intensity of light? Change the amount of GE in the jar lids?(poly-fil filters) Just keep waiting?
I was reading w a diff species of cordyceps, that once the sub took on an orange color ge was increased. Not sure by how much. Depending on the lid u have now, I'd prob increase ge in few days ish
I've never grown cordyceps
But maybe wait to see more orange once it stops darkening/turning orange I'd increase ge
Or just increase when u feel is right, could b ready now I dunno
I'm also unsure how much ge there was to begin with
"Cordyceps species always preferred low C : N ratio for growth. Highest fruit body yield and BE value of C. guangdongensis were recorded with C : N ratio of 12 : 1 (Fig. 4). The same ratio was also optimal for C. sinensis (Dong and Yao 2005), whereas a C : N ratio of 20 : 1 supported maximal biomass production (20 g l−1) and polysaccharide synthesis (6·99 g l−1) by C. militaris (Park et al. 2001). A medium containing C/N ratio of 10·8 : 1, i.e. 42·0 g l−1 glucose and 15·8 g l−1 peptone, supported high level of cordycepin production by C. militaris (Mao et al. 2005). These suggested that Cordyeps needed more nitrogen sources than other kinds of mushrooms."
Edited by Raven44 (12/11/15 02:09 PM)
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RogerSmith

Registered: 01/29/15 
Posts: 365
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Re: C. militaris endeavours [Re: Raven44]
#22642894 - 12/11/15 02:55 PM (8 years, 1 month ago) |
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It fruits fine in jar with polyfill filter More air exchange also means you want high humidity or it will dry out
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foragedfungus



Registered: 09/30/13
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Re: C. militaris endeavours [Re: RogerSmith]
#22642969 - 12/11/15 03:15 PM (8 years, 1 month ago) |
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So, I just keep waiting and cross my fingers?
When you've grown them, what is the time frame from inoculation->full colonization->orange tint->fully orange->primordia->fruits->harvest?
Last month when you posted about including a moth in the substrate, did you notice any difference vs. just grains?
Do I need to start raising caterpillars?
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RogerSmith

Registered: 01/29/15 
Posts: 365
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My cordyceps was very orange. Started white tho. Light made it turn orange. Than mybe 14days after colonization red dots-structures started appearing few days before it fruited.
Put it on light, thats all I can suggesst.
I saw no difference when I used moth. Maybe I should dry, grind and spread through whole grain and compare it this way.
I'm not growing it anymore, maybe I will try again in the future.
Caterpillars are useless. But I admit I have learned the process of growing silkworms along when I studied cordyceps online
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Raven44
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Registered: 12/07/13
Posts: 1,970
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Re: C. militaris endeavours [Re: RogerSmith]
#22644114 - 12/11/15 08:24 PM (8 years, 1 month ago) |
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Is that a tall jar in ur pic foraged fungus or?
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foragedfungus



Registered: 09/30/13
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Re: C. militaris endeavours [Re: Raven44]
#22644152 - 12/11/15 08:35 PM (8 years, 1 month ago) |
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it's a half pint regular mouth. filled about one third of the way
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Raven44
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Registered: 12/07/13
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Ahhhh, I see. I would imagine that'd be good.
Again haven't grown em..
I'd prob use a tall jar, or just a quart jar filled 1/8-1/4 of the way full. Seems that's commonly done in all the pics online...
I wish u the best of luck and if I come up w any good info I'll let u know. Cannot wait to give it a shot myself
One thing I read said they poke holes in the plastic lid of the jar when it turns orange. So I imagine they have cling wrap over the jar as a lid during colonization. Upon the jar ryening orange they poke four holes in the top. Dunno how big prob small I'd imagine, along w exhanging the air of the room once a day it said also
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foragedfungus



Registered: 09/30/13
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Re: C. militaris endeavours [Re: Raven44]
#22645526 - 12/12/15 09:31 AM (8 years, 1 month ago) |
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Thank you rogersmith and raven44, for atempting to help.
I have moved all of the jars to a spot where they get indirect sunlight all day. I loosened the amount of polyfil in the lids of 3 jars. We will see if these jars preform any differently than the rest.
I'll post any new developments here, when I have something worth sharing.
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Raven44
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Registered: 12/07/13
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Edited by Raven44 (12/12/15 06:09 PM)
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