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InvisibleRaven44
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Re: C. militaris endeavours [Re: foragedfungus]
    #22642611 - 12/11/15 01:19 PM (8 years, 1 month ago)

Quote:

foragedfungus said:
In the article you are quoting they are using pupae as substrate. Cutting open the individual cocoons, and injecting each caterpillar with a needle dipped in "spores suspended liquid"!!
That sounds like an intensive project for me to undertake.

I've seen it fruited from grains. (usually rice). Not sure what effect it will have on the compounds produced by the fungus, but it seems a little more doable for me.

I know rogersmith has had success with this species before, hopefully he will stop in with some information.



I know the quote was not very relevant, I will come w more pertinent info soon. It was interesting is all


Edited by Raven44 (12/11/15 01:31 PM)


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InvisibleRaven44
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Re: C. militaris endeavours [Re: foragedfungus]
    #22642746 - 12/11/15 02:06 PM (8 years, 1 month ago)

Quote:

foragedfungus said:
32 days ago I inoculated 12 small jars of oats with my wild C. militaris clone.
11 were fully colonized at 10-14 days. One had a spot of mold and was discarded.
In the past few days most have begun to show a slight orange color.


What is my next step towards fruiting?
Do I change the amount/intensity of light?
Change the amount of GE in the jar lids?(poly-fil filters)
Just keep waiting?



I was reading w a diff species of cordyceps, that once the sub took on an orange color ge was increased. Not sure by how much. Depending on the lid u have now, I'd prob increase ge in few days ish

I've never grown cordyceps

But maybe wait to see more orange once it stops darkening/turning orange I'd increase ge

Or just increase when u feel is right, could b ready now I dunno

I'm also unsure how much ge there was to begin with

"Cordyceps species always preferred low C : N ratio for growth. Highest fruit body yield and BE value of C. guangdongensis were recorded with C : N ratio of 12 : 1 (Fig. 4). The same ratio was also optimal for C. sinensis (Dong and Yao 2005), whereas a C : N ratio of 20 : 1 supported maximal biomass production (20 g l−1) and polysaccharide synthesis (6·99 g l−1) by C. militaris (Park et al. 2001). A medium containing C/N ratio of 10·8 : 1, i.e. 42·0 g l−1 glucose and 15·8 g l−1 peptone, supported high level of cordycepin production by C. militaris (Mao et al. 2005). These suggested that Cordyeps needed more nitrogen sources than other kinds of mushrooms."



Edited by Raven44 (12/11/15 02:09 PM)


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InvisibleRogerSmith

Registered: 01/29/15 Happy 9th Shroomiversary!
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Re: C. militaris endeavours [Re: Raven44]
    #22642894 - 12/11/15 02:55 PM (8 years, 1 month ago)

It fruits fine in jar with polyfill filter
More air exchange also means you want high humidity or it will dry out


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Invisibleforagedfungus
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Re: C. militaris endeavours [Re: RogerSmith]
    #22642969 - 12/11/15 03:15 PM (8 years, 1 month ago)

So, I  just keep waiting and cross my fingers?

When you've grown them, what is the time frame from inoculation->full colonization->orange tint->fully orange->primordia->fruits->harvest?

Last month when you posted about including a moth in the substrate, did you notice any difference vs. just grains?

Do I need to start raising caterpillars?


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InvisibleRogerSmith

Registered: 01/29/15 Happy 9th Shroomiversary!
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Re: C. militaris endeavours [Re: foragedfungus]
    #22643613 - 12/11/15 05:57 PM (8 years, 1 month ago)

My cordyceps was very orange. Started white tho. Light made it turn orange. Than mybe 14days after colonization red dots-structures started appearing few days before it fruited.

Put it on light, thats all I can suggesst.

I saw no difference when I used moth. Maybe I should dry, grind and spread through whole grain and compare it this way.

I'm not growing it anymore, maybe I will try again in the future.

Caterpillars are useless.
But I admit I have learned the process of growing silkworms along when I studied cordyceps online


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InvisibleRaven44
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Re: C. militaris endeavours [Re: RogerSmith]
    #22644114 - 12/11/15 08:24 PM (8 years, 1 month ago)

Is that a tall jar in ur pic foraged fungus or?


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Invisibleforagedfungus
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Re: C. militaris endeavours [Re: Raven44]
    #22644152 - 12/11/15 08:35 PM (8 years, 1 month ago)

it's a half pint regular mouth.
filled about one third of the way


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InvisibleRaven44
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Re: C. militaris endeavours [Re: foragedfungus]
    #22644529 - 12/11/15 10:10 PM (8 years, 1 month ago)

Ahhhh, I see. I would imagine that'd be good.

Again haven't grown em..

I'd prob use a tall jar, or just a quart jar filled 1/8-1/4 of the way full. Seems that's commonly done in all the pics online...

I wish u the best of luck and if I come up w any good info I'll let u know. Cannot wait to give it a shot myself

One thing I read said they poke holes in the plastic lid of the jar when it turns orange. So I imagine they have cling wrap over the jar as a lid during colonization. Upon the jar ryening orange they poke four holes in the top. Dunno how big prob small I'd imagine, along w exhanging the air of the room once a day it said also


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Invisibleforagedfungus
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Re: C. militaris endeavours [Re: Raven44]
    #22645526 - 12/12/15 09:31 AM (8 years, 1 month ago)

Thank you rogersmith and raven44, for atempting to help.

I have moved all of the jars to a spot where they get indirect sunlight all day.
I loosened the amount of polyfil in the lids of 3 jars. We will see if these jars preform any differently than the rest.

I'll post any new developments here, when I have something worth sharing.


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InvisibleRaven44
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Re: C. militaris endeavours [Re: foragedfungus]
    #22645700 - 12/12/15 10:41 AM (8 years, 1 month ago)

Rock on, one last post, this guy gives his formula

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/11986207


Edited by Raven44 (12/12/15 06:09 PM)


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