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Offlineforrest
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C. militaris endeavours * 1
    #22185898 - 09/03/15 11:40 PM (8 years, 4 months ago)

In the past i had a CS4 culture, but it the myc smelled very strong of ammonia. Now i have this militaris culture, it doesn't smell foul at all, it turns orange/red a bit on the bottom, and seems to be pinning (on substrate of rye and rice) but not fruiting yet.
Next to that i received another militaris culture that's originally from a South Korean company (don;t know which), so i'll be making jars of that one too this month.



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InvisibleStropharis
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Re: C. militaris endeavours [Re: forrest]
    #22187158 - 09/04/15 10:01 AM (8 years, 4 months ago)

Awesome! :rockon:


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Invisibleforagedfungus
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Re: C. militaris endeavours [Re: Stropharis] * 1
    #22189605 - 09/04/15 07:06 PM (8 years, 4 months ago)

That is awesome! Please keep us updated.
I am very interested to see your progress/results with this species, and would love any more details you can share.

I have been finding lots of C. militaris this year. I would like to try cultivating it.
After trying to clone four different fruits, I think I've finally got a clean culture going.

Here is one I found today. Going to take clones from this one too.
 


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InvisibleRogerSmith

Registered: 01/29/15 Happy 9th Shroomiversary!
Posts: 365
Re: C. militaris endeavours [Re: foragedfungus] * 1
    #22191198 - 09/05/15 01:20 AM (8 years, 4 months ago)

I wish I could find wild cordyceps too!
They look like militaris. Have you found any other cordyceps species? There are many undiscovered and exotic-looking cordyceps species.
What is the best time to look for wild cordyceps? What was the temperature of air when you found most of them? Around 20 degrees / 68 F or colder?

Fruiting militaris from fresh culture isn't hard. Just inoculate grains and put them on light once colonized.

Since we r sharing pics, I recently caught a moth, which got sterilized together with some rice. It fully colonized last week. I am wondering if any fruits will grow directly from moth.



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InvisibleToadstool5
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Registered: 01/22/15
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Re: C. militaris endeavours [Re: RogerSmith]
    #22192329 - 09/05/15 11:55 AM (8 years, 4 months ago)

:shockingsurprise:

Cordyceps porn! I really hope your subs fruit, there's nothing more awesome than in vitro cordyceps. :rockon:


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Invisibleforagedfungus
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Re: C. militaris endeavours [Re: RogerSmith] * 1
    #22192659 - 09/05/15 01:19 PM (8 years, 4 months ago)

Quote:

RogerSmith said:
I wish I could find wild cordyceps too!
They look like militaris. Have you found any other cordyceps species? There are many undiscovered and exotic-looking cordyceps species.
What is the best time to look for wild cordyceps? What was the temperature of air when you found most of them? Around 20 degrees / 68 F or colder?




The one pictured is C. militaris. Here is a thread with my cordyceps finds for this year- http://www.shroomery.org/forums/showflat.php/Number/22127308
I found all of them in upstate NY from the end of july until now (yesterday). Temps in the high 70's/low 80's(F) during the day, 60's at night.


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Offlineforrest
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Re: C. militaris endeavours [Re: foragedfungus]
    #22195378 - 09/06/15 12:50 AM (8 years, 4 months ago)

Really nice to see more people playing around with it :smile:  I'm also thinking of giving it some insects as food.


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Invisibleforagedfungus
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Re: C. militaris endeavours [Re: forrest]
    #22417421 - 10/22/15 09:45 AM (8 years, 3 months ago)

Hey Forrest, any updates for us??
I'd really like to know how this project is working out for you.

After many bacterial contam issues, I think I finally have my wild clone all cleaned up and ready for grains.
(damn it's hard to take a good picture of a no-pour dish!)


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Offlineforrest
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Re: C. militaris endeavours [Re: foragedfungus]
    #22421249 - 10/23/15 01:50 AM (8 years, 3 months ago)

jars of the second strain are colonising now, hoping they will do better.
The one i posted a picture of stalled...


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InvisibleRogerSmith

Registered: 01/29/15 Happy 9th Shroomiversary!
Posts: 365
Re: C. militaris endeavours [Re: forrest]
    #22423654 - 10/23/15 04:23 PM (8 years, 3 months ago)


:evil:


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InvisibleMrGiraffe

Registered: 04/04/14
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Re: C. militaris endeavours [Re: RogerSmith]
    #22424637 - 10/23/15 08:30 PM (8 years, 3 months ago)

Was that just rice and water with a moth?


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InvisibleRogerSmith

Registered: 01/29/15 Happy 9th Shroomiversary!
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Re: C. militaris endeavours [Re: MrGiraffe]
    #22424772 - 10/23/15 09:00 PM (8 years, 3 months ago)

Yes


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Offlineforrest
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Re: C. militaris endeavours [Re: RogerSmith]
    #22425546 - 10/24/15 01:09 AM (8 years, 3 months ago)

:cool: great


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InvisibleMrGiraffe

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Re: C. militaris endeavours [Re: RogerSmith]
    #22425827 - 10/24/15 04:43 AM (8 years, 3 months ago)

Quote:

RogerSmith said:
Yes



Where did you get your culture from?


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InvisibleRogerSmith

Registered: 01/29/15 Happy 9th Shroomiversary!
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Re: C. militaris endeavours [Re: MrGiraffe]
    #22426029 - 10/24/15 07:03 AM (8 years, 3 months ago)

I cloned a fruit


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Offlinedrake89
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Re: C. militaris endeavours [Re: RogerSmith]
    #22426477 - 10/24/15 10:10 AM (8 years, 3 months ago)

seems like most people with success fruiting get them from wild clones.


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Invisibleforagedfungus
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Re: C. militaris endeavours [Re: drake89]
    #22642121 - 12/11/15 10:24 AM (8 years, 1 month ago)

32 days ago I inoculated 12 small jars of oats with my wild C. militaris clone.
11 were fully colonized at 10-14 days. One had a spot of mold and was discarded.
In the past few days most have begun to show a slight orange color.


What is my next step towards fruiting?
Do I change the amount/intensity of light?
Change the amount of GE in the jar lids?(poly-fil filters)
Just keep waiting?


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InvisibleRaven44
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Registered: 12/07/13
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Re: C. militaris endeavours [Re: foragedfungus]
    #22642167 - 12/11/15 10:36 AM (8 years, 1 month ago)

"Troopin cordyceps (Cordyceps militaris )is also called north Chinese caterpillar fungus, north awe to in China. It belongs to Corcyceps, Clavicipitales, Pyrenomycetes, Ascomycotina, Troopin cordyceps and awe to that is widely known in China are both Corcyceps, because it's a typical breed in Cordyceps, its widely received by the scholars all over the world. The artificial cultivation of Troopin cordyceps has achieved success and a series of produces has been opened up. This will open a new route for nourishing food and functional food. In pace with development of science and technology, the cultivation and processing of Troopin cordyceps would have brilliant prospects.

As cultivating Troopin cordyceps, firstly inoculating spawn in medium to form a large number of conidiums. Secondly, choosing conidiums to make suspended spore liquid by aseptic water, then inoculate it at host, which means pupa. Protect the pupa until it become stiff. Then put the stiff pupa in simulated natural environment like wild Troopin cordyceps in it and culture it. The seed stand that grows from pupa will be exactly alike as from wild pupa.

The detailed method of operation follows:

Screening strain Rejuvenate Troopin cordyceps through live pupas to screen a spawn which sensitizer is strong and easy to form spores.
Making suspended spore liquid Inoculate the strain in medium and culture it at 20°C. When conidiums produce, choose a part of it to make suspended spore liquid by aseptic water. It will be used later.
Preparing hosts Disinfect with smoke after the silkworms go up a small bundle of straw to spin cocoons on. Cut open the cocoon and take the pupa a week later. Clean out the bad and sick pupas. Choose healthy pupas in growth phase. Or choose the five-years-old silkworms to be hosts which have not gone up a small bundle of straw to spin cocoons on.
Inoculation Sting into the pupa by an inoculation needle, which has dipped in spores suspended liquid. The rate of infection may be upon 95%.
Protecting the pupas which have been infected Spread out the pupas which have been infected in a big round shallow basket, protect the pupas to stiff at indoor temperature.
Breeding the fruit body Imitate natural environment of Troopin cordyceps. Culture the stiff pupas to form fruit body. The method is covering the stiff pupas with a coat of porous material such as bits of sponge, small piece of coal, etc. Breed the fruit body under circumstances of indoor temperature and 95% air humidity.
Directive breeding of fruit body The purpose of directive breeding of fruit body is to make the fruit body long and stiff. The method is stick the upward head of pupa into porous material such as sponge, breed it under the aforementioned conditions.
Collecting and preserving the strain of Troopin cordyceps Dig up the fruit body as it has been matured, clean it and disinfect its body with edible alcohol. Then dry it at 60°C. Seal up and store it at a cool, dark, and arid place.
Troopin cordyceps cultured in fore mentioned way, it needs 35-45 days from inoculation to mature period of the fruit body. It will harvest 50g-350g per square meter. According to examination, Cordyceps militaris artificial cultured has a great deal of awe to acid, awe to element, amino acid, trace element and alkaloid, etc. It can enrich the marrow, resist cancer, calm and diminish inflammation, stop blooding and reduce phlegm. It has a good curative effect to nephritis, hepatitis, asthma and neurasthenia."

Kinda interesting, says 35-45 days... I'll slowly be doing research myself


Edited by Raven44 (12/11/15 10:38 AM)


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Invisibleforagedfungus
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Re: C. militaris endeavours [Re: Raven44]
    #22642245 - 12/11/15 11:00 AM (8 years, 1 month ago)

In the article you are quoting they are using pupae as substrate. Cutting open the individual cocoons, and injecting each caterpillar with a needle dipped in "spores suspended liquid"!!
That sounds like an intensive project for me to undertake.

I've seen it fruited from grains. (usually rice). Not sure what effect it will have on the compounds produced by the fungus, but it seems a little more doable for me.

I know rogersmith has had success with this species before, hopefully he will stop in with some information.


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InvisibleRogerSmith

Registered: 01/29/15 Happy 9th Shroomiversary!
Posts: 365
Re: C. militaris endeavours [Re: foragedfungus]
    #22642450 - 12/11/15 12:23 PM (8 years, 1 month ago)

Can't help ya here.
Some grow, some doesn't.
Were it that easy, everyone would be growing it.


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