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Offlinemushpunx
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Letting culture hit the edge of the plate
    #22184704 - 09/03/15 08:00 PM (8 years, 4 months ago)

I was always under the impression you shouldnt let your plate fill out 100% or hit the edge of the plate. 

Is this just for storage situations or?

Or am I misinformed?

Thanks!


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InvisibleMunchauzen
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Re: Letting culture hit the edge of the plate [Re: mushpunx]
    #22184872 - 09/03/15 08:31 PM (8 years, 4 months ago)

well you want to keep the culture on the agar, where you can ensure its cleanliness. once the culture is off agar, you can no longer be certain that it is clean.


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Invisibleblindingleaf
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Re: Letting culture hit the edge of the plate [Re: Munchauzen]
    #22184979 - 09/03/15 08:47 PM (8 years, 4 months ago)

i got screwed a LOT from fridge plates stored at 100% going straight to grain.

if my plate is 100% out of fridge, i xfer to be safe.  just in past 2 weeks, i got green from doing that.  yes, i still do it. but i know full well what could happen.
i store at 50% now for cultures i like.
take out a couple days before i wanna use it
in 1-2 days, growth recovers.  i make sure its clean (paying special attention to edges of plate).  if it is, i use as if i never stored.

i got away with it for a while before.  its doable.  just know what could happen worst case scenario.

individual ziplock bags help. do what works for u.  sometimes we get screwed in the most unlikely of situations.  we grow mushrooms indoors on sterile media until bulk.  anything can happen.

also, parafilm can get dry in fridge.  always check ur seal.  things can break thru


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Offlinemushpunx
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Re: Letting culture hit the edge of the plate [Re: blindingleaf]
    #22185640 - 09/03/15 10:16 PM (8 years, 4 months ago)

Thanks for the responses guys!

Would the same go for plates you arent putting into cold storage? IE letting a plate fully grow out before putting to grain?


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InvisiblebodhisattaMDiscordReddit
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Re: Letting culture hit the edge of the plate [Re: mushpunx]
    #22185651 - 09/03/15 10:18 PM (8 years, 4 months ago)

It's best to avoid, with pasty plates its not nearly as much a concern


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InvisibleInocuole
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Re: Letting culture hit the edge of the plate [Re: bodhisatta]
    #22185655 - 09/03/15 10:19 PM (8 years, 4 months ago)

HAH!  I CAUGHT YOU ALL SAYING IT!  Where was this when everybody was encouraging newbs to do the tiger drop?


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OfflineAchillita
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Re: Letting culture hit the edge of the plate [Re: Inocuole]
    #22185912 - 09/03/15 11:47 PM (8 years, 4 months ago)

I use pasty plates, and I've had fully colonized plates sit for around 2 months before I used it(in room temperature) and it grew completely fine. No contamination or stalling whatsoever.

I'd imagine with regular agar plates that letting it grow to the edge could increase contam rate by a little bit, but I've never had that problem with pasty plates.


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Offlinemushpunx
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Re: Letting culture hit the edge of the plate [Re: Achillita]
    #22186434 - 09/04/15 04:57 AM (8 years, 4 months ago)

Right on guys

I always thought it as good practice, but didnt know for certain


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Invisiblestareatclouds
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Re: Letting culture hit the edge of the plate [Re: mushpunx]
    #22186568 - 09/04/15 06:44 AM (8 years, 4 months ago)

I thought the issue with letting the myc hit the edge was primarily for transfers. If your myc hits the edge, it can't go anywhere, allowing the slower bacteria to catch up. So when you try and transfer, you can bring bac with it easier.


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InvisibleMunchauzen
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Re: Letting culture hit the edge of the plate [Re: Inocuole]
    #22186649 - 09/04/15 07:27 AM (8 years, 4 months ago)

Quote:

stareatclouds said:
I thought the issue with letting the myc hit the edge was primarily for transfers. If your myc hits the edge, it can't go anywhere, allowing the slower bacteria to catch up. So when you try and transfer, you can bring bac with it easier.




one of many

Quote:

Inocuole said:
HAH!  I CAUGHT YOU ALL SAYING IT!  Where was this when everybody was encouraging newbs to do the tiger drop?




huh? you got beef with tiger drop? I'm about to do one right now.


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Invisibleblindingleaf
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Re: Letting culture hit the edge of the plate [Re: Munchauzen]
    #22186703 - 09/04/15 07:44 AM (8 years, 4 months ago)

i agree with bod, the pasty plates have higher wall, so if the "vector" of contamination for regular plates is the opening in close proximity to the media/mycelium (especially b/c I'm sure we have all seen the mycelium crawl up the plate wall above the media, making it even closer to the "vector"), pasty plates eliminate that vector IMO.

i have done many jars with 100% coverage that were stored in the fridge for months.  many times it works, many times it doesn't.  proceed at ur own risk.  I still do it to this day when I'm in a rush for something i need, but in addition to knocking up the grains, i will always take a small piece to another plate, because at least if grain fails, i can see that it was the culture or not.

the best bet is make 3-4 "master" plates from cultures u like, get them to 50%, then put in sandwich sized zip locks in fridge.  i don't "seal" the zip, just fold over and tape.  i know a lot of people seal it, or completely tighten their slants, but thats just how i do it cause it works for me.


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MICROBIAL HUSBANDRY!!!!

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InvisibleInocuole
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Re: Letting culture hit the edge of the plate [Re: Munchauzen]
    #22186734 - 09/04/15 07:55 AM (8 years, 4 months ago)

Quote:

Munchauzen said:
Quote:

stareatclouds said:
I thought the issue with letting the myc hit the edge was primarily for transfers. If your myc hits the edge, it can't go anywhere, allowing the slower bacteria to catch up. So when you try and transfer, you can bring bac with it easier.




one of many

Quote:

Inocuole said:
HAH!  I CAUGHT YOU ALL SAYING IT!  Where was this when everybody was encouraging newbs to do the tiger drop?




huh? you got beef with tiger drop? I'm about to do one right now.




Nah but it seems kinda weird to just use the whole damn thing after being so careful initially.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleMunchauzen
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Re: Letting culture hit the edge of the plate [Re: Inocuole]
    #22186746 - 09/04/15 07:57 AM (8 years, 4 months ago)

I used to take transfers off my tiger plates before dropping them.

these days, I have multiple plates of the same culture, so I don't have to be so frugal. The plate I just dropped, I have another one I will take transfers from and a third one thats getting given back to bb.


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Invisibleblindingleaf
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Re: Letting culture hit the edge of the plate [Re: Munchauzen]
    #22186769 - 09/04/15 08:03 AM (8 years, 4 months ago)

nice!
well i do the extra xfer as a way to "proof" the culture, even if i have it on slant or another plate.  i only do it if I'm using a fridge plate that was at 100% though.  that way, if the grain contaminates, i check the "proof" plate, and see if that is also moldy.  if it is, then the plate was bad, if it wasn't technique is to blame.  its just an extra step, not necessary, but I'm paranoid like that :eek:


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinemushpunx
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Re: Letting culture hit the edge of the plate [Re: blindingleaf]
    #22188854 - 09/04/15 04:27 PM (8 years, 4 months ago)

Quote:

blindingleaf said:
nice!
well i do the extra xfer as a way to "proof" the culture, even if i have it on slant or another plate.  i only do it if I'm using a fridge plate that was at 100% though.  that way, if the grain contaminates, i check the "proof" plate, and see if that is also moldy.  if it is, then the plate was bad, if it wasn't technique is to blame.  its just an extra step, not necessary, but I'm paranoid like that :eek:





Yea I think its worth the extra plate haha its worth being paraniod too

Contams... evvverrywhere! :eek:


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