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OfflineLogicaL ChaosM
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Re: Spore solution on agar [Re: Inocuole]
    #22212802 - 09/09/15 03:32 PM (8 years, 4 months ago)

Quote:

Inocuole said:
Lol higher potency strains from rhizomorphic growth. :rofl:

How are you such a noob if you've been here since 07 LC?




Ok, prove me wrong.

Show me a proven potent strain of mycelium that has fluffy, wispy, cotton-ish mycelium.


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Re: Spore solution on agar [Re: LogicaL Chaos] * 1
    #22212816 - 09/09/15 03:35 PM (8 years, 4 months ago)

Quote:

LogicaL Chaos said:
Quote:

Inocuole said:
Lol higher potency strains from rhizomorphic growth. :rofl:

How are you such a noob if you've been here since 07 LC?




Ok, prove me wrong.

Show me a proven potent strain of mycelium that has fluffy, wispy, cotton-ish mycelium.




pans
the growth has fuck all to do with potency it has to do with nutrition, it can go from tomentose to rhizzo with a simple recipe change


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OfflineLogicaL ChaosM
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Re: Spore solution on agar [Re: cronicr]
    #22212862 - 09/09/15 03:46 PM (8 years, 4 months ago)

Interesting...

So the food is what determines the type of mycelium growth, not the aggressive/fitness of the strain.

i was under the impression that aggressive or fit strains growth thick rhizzo mycelium which are the most potent (because they are most aggressive at colonizing a substrate).


Thanks for the explaination cronicr.


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Re: Spore solution on agar [Re: LogicaL Chaos]
    #22212868 - 09/09/15 03:47 PM (8 years, 4 months ago)

yep more nutes will make it go tomentose while less will make it go rhizzo:super:


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Re: Spore solution on agar [Re: cronicr]
    #22213228 - 09/09/15 05:02 PM (8 years, 4 months ago)



See this tub? I lost the photo of the culture plate but it was veryy aggressive, awesome looking culture. Looked freaking amazing. And the tub grew out pretty strong too.

But not potent. Pretty dissapointing actually haha.

JUst goes to show you that you need to test each and every culture out, regardless of how it looks. You might have a super fast growing isolate that puts out a crazy yeild but if it isnt potent its pretty worthless IMO


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Offlineh3aling
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Re: Spore solution on agar [Re: mushpunx]
    #22216523 - 09/10/15 11:37 AM (8 years, 4 months ago)

Heyo again,
I'm glad my thread helped you guys clear doubts, the more you know.


So I'm back with some (hopefully decent but of smartphone-shitty quality) of the incriminated plate.
Still, I don't see any of the radial patterns of mycelium seen in the other threads/pictures, but as it seems this one's totmentose rather than rhizomorphic so that's the reason, is that right?

Here are the pictures:


[I realize the camera picks up the reflection/glare of the plate and the focus on the agar is gone.]

I also come with another question.
Is it possible tha mycelium appears as a film inside a jar of liquid culture?
I suspect that is just a film of honey which solidified on the walls of the jar while I was PCing, as it slid off as I was gently swirling the jar and is now floating around happily. But if it was honey, would it not be dissolved?
I'd appreciate any input.


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Down the rabbit hole.


Edited by h3aling (09/10/15 11:39 AM)


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InvisibleMad Season
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Re: Spore solution on agar [Re: h3aling]
    #22216554 - 09/10/15 11:45 AM (8 years, 4 months ago)

That looks very contaminated to me..


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Offlineh3aling
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Re: Spore solution on agar [Re: Mad Season] * 1
    #22216620 - 09/10/15 12:05 PM (8 years, 4 months ago)

Quote:

Mad Season said:
That looks very contaminated to me..



Fuck.
You mean the white fluff is not mycelium or that the growth pattern is weird?

I came back to post this that I found googling around

The top picture looks similar to my plate.
Still, I have no real experience with agar so I might be very wrong.


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InvisibleMad Season
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Re: Spore solution on agar [Re: h3aling]
    #22216631 - 09/10/15 12:08 PM (8 years, 4 months ago)

Just all of it lol. The white growths might actually be the mycelium. Just the reaction to the (what looks like) grey shit all around it.


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlineh3aling
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Re: Spore solution on agar [Re: Mad Season]
    #22216678 - 09/10/15 12:23 PM (8 years, 4 months ago)

Quote:

Mad Season said:
Just all of it lol. The white growths might actually be the mycelium. Just the reaction to the (what looks like) grey shit all around it.



The grey shit is yellow agar but the pics came out shitty as always. You can see for reference the OP.


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InvisibleMad Season
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Re: Spore solution on agar [Re: h3aling]
    #22216707 - 09/10/15 12:30 PM (8 years, 4 months ago)

You need to read through this: http://www.shroomery.org/forums/showflat.php/Number/22020260

the moral is that if it isn't organized, don't trust it. Honestly I wasn't there the whole time that grew, so I can't say for sure. But if it was with my other dishes right now, it wouldn't be for long.

For initial transfers, don't let it grow out too much.

like at this point. If not even sooner.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (09/10/15 12:30 PM)


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Offlineh3aling
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Re: Spore solution on agar [Re: Mad Season]
    #22216932 - 09/10/15 01:33 PM (8 years, 4 months ago)

That a good thread, thank you.
So your suggestion is I transfer the mycelium to a new plate?
It does not look like contaminated anymore?


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InvisibleMad Season
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Re: Spore solution on agar [Re: h3aling]
    #22217138 - 09/10/15 02:28 PM (8 years, 4 months ago)

Try it out. If you can get it organized, then :thumbup:. I've seen crazier things done with agar.

Besides it almost looks like there's knotting. If you get a pin you most definitely should cut it out and transfer the pin


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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