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Offlinetheshitmonkey
Stranger

Registered: 08/16/15
Posts: 5
Last seen: 8 years, 4 months
Did tons of research, please tell me if this is a good idea
    #22180415 - 09/02/15 09:21 PM (8 years, 4 months ago)

So here's my Tek that I'm thinking of doing EXACTLY step by step, as specifically as possible. I haven't acted on it just yet, but I need to know if any of it is a BAD idea, I don't need unnecessary naysaying to confuse me. For example, one time someone told me not to use bubble wrap or wax paper casing because I don't need it, but if I don't need it, isn't that something I could do anyways and be okay? Some of you professional naysayers confuse me…

[ Golden Teacher ]

1. WBS

– Rinse, discard sunflower seeds and corn (mine had cracked corn, but I literally have ALL THE TIME IN THE WORLD to do this, and no vehicle atm to travel and get better stuff. Side question, could I just use plain Milo? That seems to be the main thing I see in WBS.)
– Rinse again and again, and then soak in warm water for 24 hours. Strain, rinse again and dry.
– Load into Quart jars (a bit above 500ML mark, technically at about 550). I hear a little vermiculite to catch extra moisture on the bottom doesn't hurt as well.

2. Jars

– Nail/drill hole in center lid. Tyvek on top, poly fill in the center (I heard it works better than silicone, but again PLEASE correct me if this is bad)
– Pressure cook the standard 90 minutes

3. Inoculation

– Butane lighter flame sterilize syringe. Wipe with alcohol swab.
– Stick needle into he injection port and inject 1/4 ML in 4 different spots (could 1/2 work faster or is that silly?)
– Shake every day to jangle spawn around and increase rate.

4. Bulk Substrate Prep

– Brick of Coir in 5 gallon bucket. Add one gallon (I've read it's better to do just under a gallon?) of boiling water and cover with lid for a couple hours. To capacity. Just coir, nothing else.
– Sterilize/pasteurize (I'll inquire later)

5. Colonization/Spawning to Bulk Substrate

*** I was being shifty and bought 4Qt tubs instead of 6Qt, but if ratios are still relevant and on point, it should work to a similar degree, no? I'm doing 1Qt Spawn to 2Qt Substrate, two reasons: One, It's 4Qt tub, I can't fill it but so much, and secondly the 1:2 ratio for faster colonization speed***

– Line tubs with tin foil so I can block light from sides and also so, if this is successful, I can easily take out and remove to dunk for second and third flush.
– Mix spawn and substrate thoroughly
– Cover top with tin foil, poke 4 holes with pin and cover with micro pore tape
– Wait 7-10 days, I'm thinking 10 to be sure, but I'm thinking the 1:2 ratio may not require as much time.

6. Fruiting

**Let me explain this weird fruiting chamber I'm thinking of (if it doesn't work, I'll just do normal dubtubs). The two 4Qt trays, I place them both in an even bigger plastic container (there's two of these), they both fit with some space. I do the FAE Poly fill holes in the bottom big tub and the top big tub, tight on bottom looser on top. I guess it's two tubs in a giant dub tub visually. I don't know why I want to do this but it could work, couldn't it? I'm thinking to also mist growths LIGHTLY and perhaps mist lightly the inside of the top thing to ensure humidity, and perhaps fan anyways to ensure fresh air, I don't know, forgive me if this is dumb**

– Wax paper casing, crumble up, lay on top, mist like normal, change regularly until pins come in, I may do bubble wrap instead, haven't decided.
– 12/12 5,000K Daylight a foot or two above the fruiting chamber.

7. Harvest

– I decided I will harvest as they mature/break away from the veil, only pick ones that are ready and let he rest grow. I have all the time to make sure I'm sanitary each time I interact, may even shower and be naked each time.
– Dry in food dehydrator
– And of course dunk and fruit again if it all works out.



A Few Questions:

1. On coir substrate, is it best to sterilize or pasteurize? I hear for sterilizing, you have to make sure it's in a completely sterile environment when you use it, otherwise it's prone to airborne contamination. Does pasteurized coir work better in this sense?



There it is. Sorry for fence-sittings and constant uncertainty, but I'm a total N00B. Honestly, this is just to try it out. I have all the time in the world to do it, and I spent so long researching to see what I could throw together with the least amount of money and highest probable yield (not that I expect a ton). If it works, then it'll show that I get the gyst of it and I'll try a real monotub later. But for now, as people say, this is "just for lols". I always wanted to try mushies anyways, and I figure even if it comes out shitty, at least I'll have enough for one time.

Thanks for taking time to read this if you did, and again sorry for any naivety. If none of this will work, eh, I'll just improve and try again in the future, no big deal. Thanks again!


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InvisibleGoblinKing
Trismegistus

Registered: 08/17/15
Posts: 328
Loc: Right where it belongs.
Re: Did tons of research, please tell me if this is a good idea [Re: theshitmonkey]
    #22180468 - 09/02/15 09:31 PM (8 years, 4 months ago)



Record your results and learn from them. Pasteurize your coir like so; http://www.shroomery.org/forums/showflat.php/Number/20822448


--------------------


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InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Did tons of research, please tell me if this is a good idea [Re: GoblinKing]
    #22180523 - 09/02/15 09:37 PM (8 years, 4 months ago)

Don't wipe with alcohol after flame sterilizing.. That's... I'm not even going to explain what it is, it's been said a million times.

And don't shake every day, your mycelium will never recover if you destroy it every single day.  Not using verm with the coir, custom fruiting chamber, fucking around with bubble wrap... jesus... Rather than read the rest of this I'm just gonna leave this here.

http://www.shroomery.org/forums/showflat.php/Number/19140341#19140341
http://www.shroomery.org/forums/showflat.php/Number/20541405

"Tons of research" amounts to nothing if it's not done in the right place.


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Did tons of research, please tell me if this is a good idea [Re: Inocuole]
    #22180771 - 09/02/15 10:02 PM (8 years, 4 months ago)

Research is only good if you can critically think at least as well as a 4th grader


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Offlineshellzenone
Zen
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Registered: 12/11/14
Posts: 688
Last seen: 1 year, 8 months
Re: Did tons of research, please tell me if this is a good idea [Re: bodhisatta]
    #22181089 - 09/02/15 11:04 PM (8 years, 4 months ago)

Skip the foil lining. I've heard mycellium is known to eat through it! Use a trash bag instead. It will shrink with your sub and prevent side pinning.


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OfflineMachiavelliavore
Vermiculite Hater
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Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
Re: Did tons of research, please tell me if this is a good idea [Re: theshitmonkey]
    #22181232 - 09/02/15 11:39 PM (8 years, 4 months ago)

Quote:

theshitmonkey said:
So here's my Tek that I'm thinking of doing EXACTLY step by step, as specifically as possible. I haven't acted on it just yet, but I need to know if any of it is a BAD idea, I don't need unnecessary naysaying to confuse me. For example, one time someone told me not to use bubble wrap or wax paper casing because I don't need it, but if I don't need it, isn't that something I could do anyways and be okay? Some of you professional naysayers confuse me…

[ Golden Teacher ]

1. WBS

– Rinse, discard sunflower seeds and corn (mine had cracked corn, but I literally have ALL THE TIME IN THE WORLD to do this, and no vehicle atm to travel and get better stuff. Side question, could I just use plain Milo? That seems to be the main thing I see in WBS.)
– Rinse again and again, and then soak in warm water for 24 hours. Strain, rinse again and dry.
No need to go overboard.  Just rinse it once, pull most of the sunflowers that float in big clumps by hand.  I have had no problem with the corn.  Never heard of a no simmer/boil prep.  I just boil a pot of 4x dry grain vumlume in water, dump it in and let it sit about 2hrs.
– Load into Quart jars (a bit above 500ML mark, technically at about 550). I hear a little vermiculite to catch extra moisture on the bottom doesn't hurt as well.
No need for this.  Just strain it for about 30 minutes.  If it still feels really wet, toss it in a tote in the sun and turn it over every 30 mins till it feels about right.

2. Jars

– Nail/drill hole in center lid. Tyvek on top, poly fill in the center (I heard it works better than silicone, but again PLEASE correct me if this is bad)
– Pressure cook the standard 90 minutes
I used silicon, it's fine.  AFAIK you don't need tyvek if you use polyfill though, so I'd just do that.

3. Inoculation

– Butane lighter flame sterilize syringe. Wipe with alcohol swab.  Nope, just flame it, can't get cleaner than that.
– Stick needle into he injection port and inject 1/4 ML in 4 different spots (could 1/2 work faster or is that silly?)  Since you're going to shake to inoculate the whole jar with the colonized grain from where the spores have germinated, there is no need to distribute excessively at the start.
– Shake every day to jangle spawn around and increase rate.  Each shake has a recovery time of 1-3 days.  Shake once at 15-30% colonization.

4. Bulk Substrate Prep

– Brick of Coir in 5 gallon bucket. Add one gallon (I've read it's better to do just under a gallon?) of boiling water and cover with lid for a couple hours. To capacity. Just coir, nothing else.  Buckettek is fine for coir, no other treatment required.  3.5-3.75qt water would be more appropriate for just a brick of coir with no verm IMO.
– Sterilize/pasteurize (I'll inquire later)

5. Colonization/Spawning to Bulk Substrate

*** I was being shifty and bought 4Qt tubs instead of 6Qt, but if ratios are still relevant and on point, it should work to a similar degree, no? I'm doing 1Qt Spawn to 2Qt Substrate, two reasons: One, It's 4Qt tub, I can't fill it but so much, and secondly the 1:2 ratio for faster colonization speed***  Calculate the total volume you want, then mix spawn and sub and 1:2 and distribute into the trays.  Mixing tons of spawn and sub in those will get to be a pain.  Fill appropriately.

– Line tubs with tin foil so I can block light from sides and also so, if this is successful, I can easily take out and remove to dunk for second and third flush.  Light on the sides isn't thought to trigger sidepinning anymore, as for foil liners, it could work, might get munched.  The real deal with a liner is that it sticks to and shrinks with the sub, preventing the nice sidepinning microclimate.  Not sure if foil will do that.
– Mix spawn and substrate thoroughly
– Cover top with tin foil, poke 4 holes with pin and cover with micro pore tape  no need for micropore, spawning is not remotely sterile.
– Wait 7-10 days, I'm thinking 10 to be sure, but I'm thinking the 1:2 ratio may not require as much time.
1:2 is a good ratio, and it will be fast
6. Fruiting

**Let me explain this weird fruiting chamber I'm thinking of (if it doesn't work, I'll just do normal dubtubs). The two 4Qt trays, I place them both in an even bigger plastic container (there's two of these), they both fit with some space. I do the FAE Poly fill holes in the bottom big tub and the top big tub, tight on bottom looser on top. I guess it's two tubs in a giant dub tub visually. I don't know why I want to do this but it could work, couldn't it? I'm thinking to also mist growths LIGHTLY and perhaps mist lightly the inside of the top thing to ensure humidity, and perhaps fan anyways to ensure fresh air, I don't know, forgive me if this is dumb**
So you want to put to trays in the bottom of your monotub.  Aight, as long as most of the surface area is filled, since monos rely on the sub for humidity.  Not sure why you don't just make the mono, but w.e.

– Wax paper casing, crumble up, lay on top, mist like normal, change regularly until pins come in, I may do bubble wrap instead, haven't decided.  Haven't used it, can't comment.  Nobody seems to do it currently.
– 12/12 5,000K Daylight a foot or two above the fruiting chamber.
Should be fine.  Gotten some good yields from stuff that never got any direct light.

7. Harvest

– I decided I will harvest as they mature/break away from the veil, only pick ones that are ready and let he rest grow. I have all the time to make sure I'm sanitary each time I interact, may even shower and be naked each time.  NOT A STERILE PROCEDURE, you can poke the sub, you jizz on the sub (brb, testing) and it won't matter.
– Dry in food dehydrator yes
– And of course dunk and fruit again if it all works out.  you shouldn't need to dunk at 1:2



A Few Questions:

1. On coir substrate, is it best to sterilize or pasteurize? I hear for sterilizing, you have to make sure it's in a completely sterile environment when you use it, otherwise it's prone to airborne contamination. Does pasteurized coir work better in this sense?
Read up on this lately.  The conclusion I came to is that coir doesn't contam unless you put shit spawn in it.  Heat treatments of coir are mostly to cook it and make it more edible.  Sterilizing would be the most cooking and probably the best, but buckettek is fine.



There it is. Sorry for fence-sittings and constant uncertainty, but I'm a total N00B. Honestly, this is just to try it out. I have all the time in the world to do it, and I spent so long researching to see what I could throw together with the least amount of money and highest probable yield (not that I expect a ton). If it works, then it'll show that I get the gyst of it and I'll try a real monotub later. But for now, as people say, this is "just for lols". I always wanted to try mushies anyways, and I figure even if it comes out shitty, at least I'll have enough for one time.

Thanks for taking time to read this if you did, and again sorry for any naivety. If none of this will work, eh, I'll just improve and try again in the future, no big deal. Thanks again!

One final note, nothing you do after you have fully colonized spawn is sterile, and it can all be done with little care.





--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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