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Offlinedonkeycong
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3 Monotub contam in a row!!!
    #22179476 - 09/02/15 06:29 PM (8 years, 4 months ago)

All of my tubs do perfect and colonize 100% in 4-7 days, but right when i replace the tape with polyfill, mushrooms start to grow, along with contams of all sorts. I was under the impression that substrates colonized 100% were pretty much contam resistant. What do you guys think!?





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InvisiblePirateSwazey
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Re: 3 Monotub contam in a row!!! [Re: donkeycong]
    #22179482 - 09/02/15 06:30 PM (8 years, 4 months ago)

I think you got dirty spawn problems


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Offlineshellzenone
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Re: 3 Monotub contam in a row!!! [Re: donkeycong]
    #22179490 - 09/02/15 06:31 PM (8 years, 4 months ago)

There might be a bacterial contamination in your spawn. This usually weakens your mycellium and it becomes prone to contamination.


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OfflineBig Worm
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Re: 3 Monotub contam in a row!!! [Re: donkeycong]
    #22179495 - 09/02/15 06:32 PM (8 years, 4 months ago)

There looks like a lot of moisture in those tubs IMO.  Which would cause contams to thrive.


I'm no expert though, so I would definitely look for some other responses as well.


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InvisiblePirateSwazey
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Re: 3 Monotub contam in a row!!! [Re: Big Worm]
    #22179505 - 09/02/15 06:33 PM (8 years, 4 months ago)

If your coir sub contams pre first flush 99% of the time it is bad spawn.

Want to fix the problem? Inoculate w agar and not spores or LC.


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Offlinedonkeycong
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Re: 3 Monotub contam in a row!!! [Re: PirateSwazey]
    #22179531 - 09/02/15 06:38 PM (8 years, 4 months ago)

Could be, doubt it though, my spawn jars always look and smell on point. I was thinking a dusty fan I oscillate on the tubs might be blowing contams through the polyfill, but i had a very successful grow with the same fan right before i started having contamination problems.


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OfflineMoxyOx
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Re: 3 Monotub contam in a row!!! [Re: donkeycong]
    #22179549 - 09/02/15 06:41 PM (8 years, 4 months ago)

You sure those polyfill holes can breathe?

They look extremely tight. You might just have so much stale air building up, combined with the moisture, that it's choking your mycelium. Thus, the trich.


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OfflinePsilosopherr
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Re: 3 Monotub contam in a row!!! [Re: MoxyOx]
    #22179579 - 09/02/15 06:45 PM (8 years, 4 months ago)

And your plastic liner is not see through. So the bottom might be uncolonized :shrug:


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Offlinedonkeycong
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Re: 3 Monotub contam in a row!!! [Re: MoxyOx]
    #22179599 - 09/02/15 06:48 PM (8 years, 4 months ago)

A possibility, I dialed in using franks method but maybe made the bottom ones too tight. The stale air theory is intriguing as i always turn the fan off at night so I can sleep(its in my room and loud af) and sometimes forget to turn it back on before i leave for work. The mycelium might not be getting enough air to stay strong and fend off contamination


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InvisibleMad Season
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Re: 3 Monotub contam in a row!!! [Re: donkeycong]
    #22179675 - 09/02/15 07:06 PM (8 years, 4 months ago)

Quote:

donkeycong said:
Could be, doubt it though, my spawn jars always look and smell on point. I was thinking a dusty fan I oscillate on the tubs might be blowing contams through the polyfill, but i had a very successful grow with the same fan right before i started having contamination problems.



I don't get why people are always so against dirty spawn. It's the fuckin hardest part of the hobby. It took me 5 years to figure out how to have clean spawn. You can make a tub of pasteurized coir (No spawn). Leave it in the tub with stuffed poly to any level of tightness, and it won't contaminate for months. Add spawn, and suddenly it contaminates..

There's hidden contams.. Almost all of those would look clean on grains, and smell good in grains. That's why you need to do agar.

There's also  bacteria. (More bacteria), all of which also smelled mushroomy when spawning.


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Invisibletahoe
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Re: 3 Monotub contam in a row!!! [Re: donkeycong]
    #22179689 - 09/02/15 07:09 PM (8 years, 4 months ago)

It's either dirty spore syringe, dirty culter, improper grain sterilization, bad inoculation technique,  bad coir, or improper coir pasteurization.
I would start with the spore syringe if being used. I would bet you are introducing it to the coir. 4-7 days is about the time it takes to flourish.


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Invisibletahoe
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Re: 3 Monotub contam in a row!!! [Re: tahoe]
    #22179699 - 09/02/15 07:11 PM (8 years, 4 months ago)

Hey mad. I have never had good luck with rye. It always contaminates on me. I am currently trying rye again. I did 5 hour 22psi cook times on it. We shall see


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Offlineshellzenone
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Re: 3 Monotub contam in a row!!! [Re: Mad Season]
    #22179717 - 09/02/15 07:14 PM (8 years, 4 months ago)

Quote:

There's hidden contams.. Almost all of those would look clean on grains, and smell good in grains. That's why you need to do agar.

There's also  bacteria. (More bacteria), all of which also smelled mushroomy when spawning.





This


Why do you make a post then deny the advice you get? Do you want us to tell you it's something else?


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InvisibleMad Season
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Re: 3 Monotub contam in a row!!! [Re: tahoe]
    #22179718 - 09/02/15 07:15 PM (8 years, 4 months ago)

Rye around this time of year is bottom of the barrel endospores attack. :frown: I bet that pc time and pressure really cut down their numbers. Realistically, the endospores should have died, so it should be clean :lol:. :goodluck:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinedonkeycong
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Re: 3 Monotub contam in a row!!! [Re: shellzenone]
    #22179756 - 09/02/15 07:24 PM (8 years, 4 months ago)

Dude I didn't deny the advice, i said it was a possibility. I just didn't think that was the case b/c my spawn grew vigorously and looked extremely healthy. You would think that contams would hinder growth or h ave some sort of effect, even if hidden. I don't know much about hidden contamination, and regarding Mad's statement on why people are so againt dirty spawn, I think people just don't believe/consider things they cant detect with their senses. This isn't right or wrong, its just the way the brain works.


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Offlineshellzenone
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Re: 3 Monotub contam in a row!!! [Re: donkeycong]
    #22179777 - 09/02/15 07:29 PM (8 years, 4 months ago)

You're right ok, didn't mean to sound harsh. It's just that I keep  seeing a lot of these threads and everyone swears it's not their spawn. Even I had the same problem and didn't believe it was my spawn. I had to throw out my master cultures and start over from spores, but it was the best desicion I made.


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Offlinedonkeycong
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Re: 3 Monotub contam in a row!!! [Re: shellzenone]
    #22179808 - 09/02/15 07:36 PM (8 years, 4 months ago)

Its all good bro were cool! :cool: Im gonna start a new batch of jars from spores and do a little agar work on the side. Wish me luck guys!


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InvisibleMad Season
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Re: 3 Monotub contam in a row!!! [Re: donkeycong]
    #22179849 - 09/02/15 07:45 PM (8 years, 4 months ago)

Word. Honestly contams may grow at the same rate, and look exactly the same. I can think of 5 different contam GENUS'S (that's like hundreds of species of molds) off the top of my head that'd look exactly like cubes in jars, and grow at the same rate. And as you've experienced, they end up showing themselves when you spawn to bulk. 1 bad jar can ruin a tub. It's shitty but it's true.

All spores came from a mushroom cap that fruited and opened up in open air. It's almost impossible to make a clean print (and in turn any spore syringe, vendor syringe included). It's unfortunate but agar is really the only way to get around the bullshit. Fuckin 200,000 mold spores per inch in the air (on average) and 500,000 per mm on your skin. That's the shit we're fighting against :lol:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (09/02/15 07:46 PM)


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InvisiblePirateSwazey
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Re: 3 Monotub contam in a row!!! [Re: Mad Season]
    #22179864 - 09/02/15 07:50 PM (8 years, 4 months ago)

Remember just because it's white don't mean it's right...

Green mold has white myc too & it loves to tangle up with the good shit.


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BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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