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Greenganja
Friendly Stranger


Registered: 08/30/15
Posts: 20
Loc: Ontario Canada
Last seen: 7 years, 8 months
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Agar start and first grow
#22179403 - 09/02/15 06:16 PM (8 years, 4 months ago) |
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Hey their trying to get my first grow underway. Have some bags going now but my pc was messed up so don't think they are going to work so am going to make some agar plate when my new syringe gets here in about a week. Here's my 4 agar containers. 150ml-ish jars with silicone injection port and painter suit for air exchange.

Here's my ghetto SAB

With a plastic drop sheet taped to front, and wet soapy towel underneath. wash everything , spray with 70% iPa wait a few mins , put on tyvek sleeves and gloves and face mask spray myself down. Then spray with distilled water inside so surface is wet and give a little Lysol spray inside and wait another minute.
Sound acceptable am I missing anything or doing something in wrong step?Do that to the jars and everything too while inside already of course
-------------------- And then one day you find ten years have got behind you No one told you when to run, you missed the starting gun
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 11 days, 3 hours
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Re: Agar start and first grow [Re: Greenganja]
#22179520 - 09/02/15 06:36 PM (8 years, 4 months ago) |
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Quote:
Greenganja said: Hey their trying to get my first grow underway. Have some bags going now but my pc was messed up so don't think they are going to work so am going to make some agar plate when my new syringe gets here in about a week. Here's my 4 agar containers. 150ml-ish jars with silicone injection port and painter suit for air exchange.

Here's my ghetto SAB

With a plastic drop sheet taped to front, and wet soapy towel underneath. wash everything , spray with 70% iPa wait a few mins , put on tyvek sleeves and gloves and face mask spray myself down. Then spray with distilled water inside so surface is wet and give a little Lysol spray inside and wait another minute.
Sound acceptable am I missing anything or doing something in wrong step?Do that to the jars and everything too while inside already of course
If your pressure cooker is down, how do you plan on doing agar? Maybe I misread something. Since you're going MS syringe > agar with a SHIP, you're sterile procedure is fine. A little more than necessary with SHIPs, but never hurts to get as clean as you can get. Doesn't get much cleaner than a flaming red needle being inserted into a SHIP as far as no SAB (True SAB) is concerned. A lot of people will say ditch the ships, and just build a SAB. Not saying you dont have one, its just not quite a proper "still air" box. I'm a fan of both SHIPS and working with scalpel. I occasionally use ships, but prefer popping lids open to drop spores. If you're not using a SAB and wanting to do agar, I would highly suggest it, otherwise you'll be stuck to using ships, or will have very high contam rates. And even then when you go from agar to master, you want to do this in a SAB, it's a lot harder to get myc back into a syringe from an agar with a SHIP.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
Edited by iSmkGrnBud (09/02/15 06:41 PM)
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
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Re: Agar start and first grow [Re: iSmkGrnBud]
#22179555 - 09/02/15 06:41 PM (8 years, 4 months ago) |
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Yeah that's a pretty bad SAB. You're not stilling any air in there brotha. Buy a tote, heat up a knife and cut. You get through plastic like butter.
Or buy a hole saw set for like 20 bucks.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Greenganja
Friendly Stranger


Registered: 08/30/15
Posts: 20
Loc: Ontario Canada
Last seen: 7 years, 8 months
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Re: Agar start and first grow [Re: iSmkGrnBud]
#22179562 - 09/02/15 06:43 PM (8 years, 4 months ago) |
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Sorry my pressure cooker was down for the first bags I think are going to be contained but now I have a working one. And I'm planning to innco those 4 agar with my spore syringe and then use my hopefully good dish I may have for bag/jars/agar transfers in my rigged SAB if you think I could get away with what I made
-------------------- And then one day you find ten years have got behind you No one told you when to run, you missed the starting gun
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Greenganja
Friendly Stranger


Registered: 08/30/15
Posts: 20
Loc: Ontario Canada
Last seen: 7 years, 8 months
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Re: Agar start and first grow [Re: Greenganja]
#22179574 - 09/02/15 06:44 PM (8 years, 4 months ago) |
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And what is so much different than the one I made? Do you think the hand wholes need to be smaller. I will be putting a planting sheet to cover more when working too
-------------------- And then one day you find ten years have got behind you No one told you when to run, you missed the starting gun
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Agar start and first grow [Re: Greenganja]
#22179580 - 09/02/15 06:46 PM (8 years, 4 months ago) |
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Your hand hole is a big ass gap in the wall, that's a pretty big difference from an SAB that has two small holes in it. Air can literally move in and out of that with 0 problems. I think if you're going to modify that, buy a real, dedicated SAB tote
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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iSmkGrnBud
Psychonaut



Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 11 days, 3 hours
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Re: Agar start and first grow [Re: Greenganja]
#22179602 - 09/02/15 06:50 PM (8 years, 4 months ago) |
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Quote:
Greenganja said: Sorry my pressure cooker was down for the first bags I think are going to be contained but now I have a working one. And I'm planning to innco those 4 agar with my spore syringe and then use my hopefully good dish I may have for bag/jars/agar transfers in my rigged SAB if you think I could get away with what I made
I dont think that SAB is proper for agar. The worst you could do is try though. Set that SAB on a clean towel so it seals the open bottom of the SAB.
http://www.shroomery.org/forums/showflat.php/Number/20048771#20048771
Here's a great SAB tek. Doesnt need to look as fancy, but should follow the basic principles. I would recommend a way larger SAB, you want a lot of room to work with.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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Greenganja
Friendly Stranger


Registered: 08/30/15
Posts: 20
Loc: Ontario Canada
Last seen: 7 years, 8 months
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Re: Agar start and first grow [Re: iSmkGrnBud]
#22179637 - 09/02/15 06:57 PM (8 years, 4 months ago) |
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I guess had a bunch of these I can use from work so thought I'd try couldn't really put handholes in and still be able to move around food. I'll look for a bigger one. I was already trying to kind of follow that tek
-------------------- And then one day you find ten years have got behind you No one told you when to run, you missed the starting gun
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Agar start and first grow [Re: Greenganja]
#22179658 - 09/02/15 07:02 PM (8 years, 4 months ago) |
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i mean…its not the worst. but if u just starting, not the safest unless u know wha ur doing movement wise (slow, steady, confident and deliberate movements) kizzle uses a similar design with a rectangle cut out instead of arm holes. ur agar dishes will tell u the whole story though, don't worry.
going agar>bag…not sure that is best way, although people do it. jars are really awesome in an SAB like that. really can't beat the ease, safety and speed. bags have their place, but they have their risks too.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
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Re: Agar start and first grow [Re: taGyo]
#22179681 - 09/02/15 07:07 PM (8 years, 4 months ago) |
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Quote:
taGyo said: Your hand hole is a big ass gap in the wall, that's a pretty big difference from an SAB that has two small holes in it. Air can literally move in and out of that with 0 problems. I think if you're going to modify that, buy a real, dedicated SAB tote 
I think it can work just fine, depends on how much you move your arms when you work.
Quote:
Kizzle said: I found the most significant disturbances were caused by vibrations from arms moving against the arm holes. So I removed the arm holes altogether and just have one side half open. Of course the room I use my SAB has very still air to begin with so protecting it from external currents isn't particularly important. I'm mainly just protecting it from me hovering over it while I work.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Agar start and first grow [Re: GreenRabbit]
#22179693 - 09/02/15 07:10 PM (8 years, 4 months ago) |
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as long as it's in a fairly still room, that box should be ace. Better than small arm holes actually.
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Greenganja
Friendly Stranger


Registered: 08/30/15
Posts: 20
Loc: Ontario Canada
Last seen: 7 years, 8 months
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Re: Agar start and first grow [Re: Mad Season]
#22179746 - 09/02/15 07:21 PM (8 years, 4 months ago) |
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Ok that's what I was thinking kinda smaller holes would make suction or hitting or something. And yea tried bags the first for some reason gonna make some jars to go next probably bags seemed too risky
-------------------- And then one day you find ten years have got behind you No one told you when to run, you missed the starting gun
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
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Re: Agar start and first grow [Re: Greenganja]
#22179851 - 09/02/15 07:47 PM (8 years, 4 months ago) |
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Just pretend there are holes when you move your arms, and have the air in the room as still as possible as well.
By that, I mean move your arms in and out; not in a swinging motion.
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