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trippinfloriduh
regnarts


Registered: 02/22/12
Posts: 87
Loc: Floriduh
Last seen: 1 year, 10 months
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LC Lid help Please
#22175067 - 09/01/15 06:16 PM (8 years, 4 months ago) |
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I'm getting ready to take the last of a MS syringe and make some LC. I have read countless teks and would like to get some opinions. I do not wish to go "hi-tech" with the lids yet. I just want to keep it real simple. Here is what I plan to do after the reading I have done.
I am going to drill two 1/4 inch holes...one in the center and one to the outer edge.
Silicone a piece of tyvek from an envelope to the top of the lid. (Should I silicone a piece to the bottom side as well?)
Silicone the outer hole bottom and top side fairly thick on both sides for my injection site.
Any input would be greatly appreciated!!!!
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"Whether drugs lead to illumination or degradation depends on the spirit in which one takes them."
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2shoes
The anti-agar



Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB
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MS > LC is risky at best. I'd say poly fill the hole in the lid. Tyvek isn't that good.
I would use a sfd and a true ship vs silicone but that's just me.
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: LC Lid help Please [Re: 2shoes]
#22175136 - 09/01/15 06:36 PM (8 years, 4 months ago) |
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Whatever you do don't noc up LC with spores.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Bro, this whole idea is botched. Spores into an LC is a very bad method and will more than likely result in contamination. Tyvek, when wet, will act more as a window than a filter. So, to shake your LC, which you will need to do, you are going to get your filter soaking wet.
There is no "simple mode" for LCs. You either do them correctly or fail.
You will need whatman filters. These are special style filters that won't get wet when you shake your jar. You will also need to learn agar so you can inoculate your LC with a clean wedge, not some dirty spores.
I'm sure you can find a better usage of your materials than this crapshoot of a plan.
This is coming a from a guy who spent a year making LCs from homemade lids. You do not want to go down this path!
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2shoes
The anti-agar



Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB
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I would recommend "thinning" your spores with sterile water before going LC. How many CC's do you have?
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trippinfloriduh
regnarts


Registered: 02/22/12
Posts: 87
Loc: Floriduh
Last seen: 1 year, 10 months
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Re: LC Lid help Please [Re: 2shoes]
#22175242 - 09/01/15 07:01 PM (8 years, 4 months ago) |
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I have about 3-4 cc's left.....I do have some birdseed 95% colonized. Could I scrape some myc from one of the jars in a still air box?
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"Whether drugs lead to illumination or degradation depends on the spirit in which one takes them."
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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...Are you implying my LC needs to breath?
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trippinfloriduh
regnarts


Registered: 02/22/12
Posts: 87
Loc: Floriduh
Last seen: 1 year, 10 months
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Re: LC Lid help Please [Re: YaMoonSun]
#22175276 - 09/01/15 07:08 PM (8 years, 4 months ago) |
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Why would you ask? It seems every tek I have read says to have a filter port.
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"Whether drugs lead to illumination or degradation depends on the spirit in which one takes them."
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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trippinfloriduh
regnarts


Registered: 02/22/12
Posts: 87
Loc: Floriduh
Last seen: 1 year, 10 months
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Re: LC Lid help Please [Re: YaMoonSun]
#22177023 - 09/02/15 04:45 AM (8 years, 4 months ago) |
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Thanks.....giving her a whirl this way...using a homemade filter syringe.
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"Whether drugs lead to illumination or degradation depends on the spirit in which one takes them."
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