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Ukgrade
Stranger
Registered: 08/30/15
Posts: 6
Last seen: 8 years, 4 months
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A few noob questions before I attempt my 2nd grow!
#22174029 - 09/01/15 01:27 PM (8 years, 4 months ago) |
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I've just threw out my last grow due to contamination& I'm hoping this next grow is going to be contam free! I'm going to flame sterilise the needle before i inoculate this time but how long after heating should i wait before inoculating? & should i flame before each 4 inoculation points or just the once? i'm going to put the pot inside another plastic container cleaned with lysol or something similar and fruiting is meant to be done in this transparent bag provided (according to the grow kit) I'm wondering if any contams could possibly get to the substrate from the bag or should i be good? thanks!
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Re: A few noob questions before I attempt my 2nd grow! [Re: Ukgrade]
#22174040 - 09/01/15 01:30 PM (8 years, 4 months ago) |
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Personally, I flame sterilize the needle, and cool the needle with a bit of spore solution right before inoculating four times. I only flame sterilize in between jars, not each inoculation. If you want to you probably can, but I don't want to waste more spore than I have to. Only takes 1/8 of a ml to cool the needle.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: A few noob questions before I attempt my 2nd grow! [Re: Ukgrade]
#22174045 - 09/01/15 01:31 PM (8 years, 4 months ago) |
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what kind of grow kit is this? fruiting in bags is generally a bad idea. www.mushroomvideos.com shows you how to do it properly.
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: A few noob questions before I attempt my 2nd grow! [Re: spacechildo]
#22174068 - 09/01/15 01:39 PM (8 years, 4 months ago) |
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Just flame sterilize, wait a few seconds, and squirt the solution into the jars. 1/4cc in each hole, all 4 holes. 1cc total per jar. The first couple drops leaving the syringe will cool the needle enough.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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TheShroomanizer
Stranger-Danger


Registered: 06/12/09
Posts: 1,571
Loc: The Swamp
Last seen: 9 months, 1 day
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Re: A few noob questions before I attempt my 2nd grow! [Re: Buck513]
#22174088 - 09/01/15 01:45 PM (8 years, 4 months ago) |
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Yes I also flame sterilize red hot before each different jar. And right after I flame the needle I shake the syringe...you can hear a little popping...which is the solution entering the syringe and cooling it down. Then I inject. Doesn't take long for it to cool down
-------------------- Trading Prints -Nature gave us one tongue and two ears so we could listen twice as much as we speak-
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Ukgrade
Stranger
Registered: 08/30/15
Posts: 6
Last seen: 8 years, 4 months
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Re: A few noob questions before I attempt my 2nd grow! [Re: TheShroomanizer]
#22176819 - 09/02/15 02:10 AM (8 years, 4 months ago) |
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Sweet thanks for the replies as for the grow kit this is what I have http://postimg.org/image/anhh26su3/ And the fruiting was meant to be done in this bag http://postimg.org/image/ez2m1eu2v/ Do you think that would be okay to fruit in or should I fruit in something else, not sure if the bag being exposed to air could possibly cause contams if so what do you recommend to use? Thanks!
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: A few noob questions before I attempt my 2nd grow! [Re: Ukgrade]
#22176822 - 09/02/15 02:14 AM (8 years, 4 months ago) |
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I would sterilize between jars, at most. Between holes is just silly and wasteful. I would do every third jar now if I did the pf tek
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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