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Offlinesnooteroid
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white specks. yeast contamination?
    #22173457 - 09/01/15 10:43 AM (8 years, 4 months ago)

Hey everyone I believe this is my first post on shroomery
I've got to say thanks to everybody because Ive learned so much.

Anyway these containers are meant to go into fruiting today but they have these strange white specks.
Are they contaminated?
I know that if they are I should throw them out but it would kill me to toss so much mycelium, is there anyway to salvage these lil cuties?


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Edited by snooteroid (06/16/16 01:26 PM)


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Offlineconfuzzed
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Re: white specks. yeast contamination? [Re: snooteroid]
    #22173780 - 09/01/15 12:13 PM (8 years, 4 months ago)

http://http://www.shroomery.org/5276/What-are-common-contaminants-of-the-mushroom-culture

Are these Cubes?
I have seen oysters make small mycelial dots, but not cubes... and this being on the sides of the container probably means its colonizing the sugars in the water... It does look like a contaminant to me.
Yeast will usually bring a fowl odor when not brewed up in a liquid... and even in a liquid culture it gets very stinky quite often...

since you were going to fruit them today, try taking a picture of it being outside of the container.  As you were planning on doing this anyway i dont see how it could hurt.

If it is yeast or a budding microbe similar to yeast you will not be able to save it. Keeping it around will only make matters worse.

Try taking a non-container pic maybe that will help.
As of now i cant tell u what it is. Nor does it look like something i would want to fool with.


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Offlinesnooteroid
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Re: white specks. yeast contamination? [Re: confuzzed]
    #22173801 - 09/01/15 12:21 PM (8 years, 4 months ago)

Thanks for the reply
Yes they are cubes

With the tek I'm using I only remove the lid and put them in the greenhouse, so I imagine it would not help visually as the spots seem to only be on the inside, and of course I would be worried to put them next to my healthy containers.  I suppose I'll just see of anyone else replies and if not I'll throw em out


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InvisibleMad Season
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Re: white specks. yeast contamination? [Re: snooteroid]
    #22177353 - 09/02/15 08:27 AM (8 years, 4 months ago)

I've seen cubes do that a lot. It's called knotting :lol: it's healthy. Doesn't look at all yeasty.


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Offlineconfuzzed
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Re: white specks. yeast contamination? [Re: Mad Season]
    #22177891 - 09/02/15 11:50 AM (8 years, 4 months ago)

Quote:

Mad Season said:
I've seen cubes do that a lot. It's called knotting :lol: it's healthy. Doesn't look at all yeasty.




Knotting like that before full colonization? I can still see some un colonized seed in there.
It looks like beginning of white pin head-like mold I get if I keep my bird seed container open when it rains :mypleasure:

It usually congregates most where the water sits. 
I haven't seen knotting on the container like that but I could be wrong.

Edit: i do agree it's not yeast!


Edited by confuzzed (09/02/15 11:51 AM)


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InvisibleMad Season
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Re: white specks. yeast contamination? [Re: confuzzed]
    #22177894 - 09/02/15 11:51 AM (8 years, 4 months ago)

It's a bit wet, but it's fully colonized. It looks really good actually imo


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Edited by Mad Season (09/02/15 12:33 PM)


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Offlinesnooteroid
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Re: white specks. yeast contamination? [Re: Mad Season]
    #22178626 - 09/02/15 03:22 PM (8 years, 4 months ago)

I opened it and it smells fine.  I put it in the greenhouse but should I take it back out?


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Offlineconfuzzed
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Re: white specks. yeast contamination? [Re: snooteroid]
    #22178746 - 09/02/15 03:48 PM (8 years, 4 months ago)

Quote:

snooteroid said:
I opened it and it smells fine.  I put it in the greenhouse but should I take it back out?




If it smells it has to be pretty far gone.

Mad Season seems to think they're alright.
I'd go with his advice.


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Offlinesnooteroid
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Re: white specks. yeast contamination? [Re: confuzzed]
    #22192406 - 09/05/15 12:18 PM (8 years, 4 months ago)

Update: it's very very bad!
It has become an epidemic, I've lost 7 containers, over half, it contaminated all the ones in the greenhouse due to my insolence and it has appeared on unopened containers that have been colonizing quite healthily for a month, the fully colonized substrate seems to have no defense against its infectious power
The mission is fubar


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Offlineconfuzzed
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Re: white specks. yeast contamination? [Re: snooteroid]
    #22192665 - 09/05/15 01:20 PM (8 years, 4 months ago)

That's not good man :frown:!!!

This is why I/we don't like to take chances.
When you have so much going it's not worth it to foul up all your efforts, by trying to squeeze by something that's unsure...

A lot of people do what they want anyway and then smash people on here for being "contamination nazis" when they get fruits, but in this case our advice didn't save you from this mess...

Sorry man. Can I ask how your keeping your culture around?
You definitely need to separate everything from your culture so your culture itself doesn't get contaminated! If it's not already.. It may be the source... If you post something of what it looks like someone may be able to help you pin point the source!

Is it sporulating? Like a pin-head like mold?
Has it changed colors? Smell?

You definitely have a large decontam that is needed.
Determining the source may be your best bet to keeping it from happening in the future.

Keep us posted!


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Offlinesnooteroid
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Re: white specks. yeast contamination? [Re: confuzzed]
    #22198230 - 09/06/15 04:54 PM (8 years, 4 months ago)

Hey thanks for the reply!

They were ms syringe inoculated
I have no cultures yet.

I think it has a smell now, it was vaguely unpleasant like feet but it was so subtle it was hard to notice. 

I've got some pictures to share

This is the bigger container that was originally shown
It doesn't look too much worse but it is clearly diseased beyond redemption

This one seemed fine although it seemed to have less dense mycelium I now realize it was surely infected the whole time, however the spots only showed up within the last week


Here it is next to healthy mycelium, you can clearly see the difference

Well technically it isn't healthy mycelium
It was sadly contaminated by that container due to being with it in the greenhouse
I left it outside to see if it could still fruit but overnight it has become covered with hundreds of spots,when I realized it was contaminated the day before it had only a few
The thick white mycelium is fading

This picture is a penis envy inoculated by syringe the same night the square container was inoculated by grain to grain
The contaminate grew out of control before the spores could germinate
You can see it is crusty and flaky looking
Very nasty


Since these pictures were taken I've sterilized all the contaminated containers and dumped them outside
I also meticulously cleaned the greenhouse with Lysol and air disinfectant as well

Anything else I can do to prevent further spread?

I've still got a few containers holding on


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Edited by snooteroid (06/16/16 01:28 PM)


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InvisibleMad Season
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Re: white specks. yeast contamination? [Re: snooteroid]
    #22198291 - 09/06/15 05:13 PM (8 years, 4 months ago)

:facepalm: mycelium isn't always pure white. Sometimes it's darker. That still is mycelium. Last pic is bacterial (smell it. Smells disgusting) but I bet if you smelled the others, they'd be mushroomy.

This is cobweb:




See how it's fuzzy with no visible rizmorphs? It's extremely cottony and fluffy. Also darker grey.

Next time take pics of the surface of the substrates. It doesn't matter if you do it in open air. Colonized substrates are able to fight against contams.

Is there any other reasons besides being a different colour that'd make you call it diseased?


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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Edited by Mad Season (09/06/15 05:14 PM)


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Offlinesnooteroid
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Re: white specks. yeast contamination? [Re: Mad Season]
    #22198425 - 09/06/15 05:46 PM (8 years, 4 months ago)

It's not the color it's the white dots all over it, they're most visible on the square container but they are present on the others as well.  I know it isn't mycelium because the exact same white dots appeared in the Container in the last picture before growing into the flaky crusty stuff you see


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InvisibleMad Season
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Re: white specks. yeast contamination? [Re: snooteroid]
    #22198549 - 09/06/15 06:12 PM (8 years, 4 months ago)

It's bacterial. The white stuff is actually the mycelium. It just isn't growing due to the bacteria.

Those white "spots" are still mycelium/mushrooms. If it smells like shit, then maybe I'd say it's contamination, but I'd bet that is still mushroomy smelling and still healthy. I've seen it do it a lot. Like literally every other grow


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleToadstool5
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Re: white specks. yeast contamination? [Re: Mad Season]
    #22205059 - 09/07/15 11:01 PM (8 years, 4 months ago)

They do look like knots to me but i have yet to see a yeast infection.

I was taught that with yeast the dots will stay about the size of a grain of sand and spread VERY rapidly from the initial colony.

With knots they will get larger and start all over the substrates even on the bottom and sides rather than from a localized point. I have had so many pop up in a small area and so dense i initially thought it was mold but alas it was a good cluster. :lol:

:goodluck: next time you get some, keep it and wait a few days to see if they get larger and start to form primordia.


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