|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Greenganja
Friendly Stranger


Registered: 08/30/15
Posts: 20
Loc: Ontario Canada
Last seen: 7 years, 8 months
|
|
The seals on the plastic ones broke so ended up making 4 of these glass 150ml-ish ones. silicone for injection port tyveklike material for air exchange
-------------------- And then one day you find ten years have got behind you No one told you when to run, you missed the starting gun
Edited by Greenganja (09/01/15 10:53 PM)
|
Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
|
|
Is that kite-making tyvek, I've used them before, they work pretty well. But, SFD's are at the top of the list when it comes to best GE filter.
And instead of using SHIP's, why not just crack the lid in a SAB. Your gonna have to get used to sterile techniques, especially if you want to start working with agar
|
Greenganja
Friendly Stranger


Registered: 08/30/15
Posts: 20
Loc: Ontario Canada
Last seen: 7 years, 8 months
|
|
Like I said before a needle just seems more sterile after having to pc why open before i need to and have any risk. And no it's this Bennett coveralls I found at lowes I couldn't find tyvek anywhere but this says does 1 micron filtering and everything I've ready says 3 and up is the danger zone if I'm correct?
-------------------- And then one day you find ten years have got behind you No one told you when to run, you missed the starting gun
|
Greenganja
Friendly Stranger


Registered: 08/30/15
Posts: 20
Loc: Ontario Canada
Last seen: 7 years, 8 months
|
|
Here's my ghetto SAB

With q plastic drop sheet taped to front, and wet soapy towel underneath. wash everything , spray with 70% iPa wait a few mins , put on tyvek sleeves and gloves and face mask spray myself down. Then spray with distilled water inside so surface is wet and give a little Lysol spray inside and wait another minute.
Sound acceptable am I missing anything or doing something in wrong step?Do that to the jars and everything too while inside already of course
-------------------- And then one day you find ten years have got behind you No one told you when to run, you missed the starting gun
Edited by Greenganja (09/02/15 03:52 PM)
|
Greenganja
Friendly Stranger


Registered: 08/30/15
Posts: 20
Loc: Ontario Canada
Last seen: 7 years, 8 months
|
|
Ok gonna start a new post guesse abandoning this bag one probably
-------------------- And then one day you find ten years have got behind you No one told you when to run, you missed the starting gun
|
Munchauzen


Registered: 06/22/11
Posts: 14,342
|
|
heres a video of me preparing my sab. you are going pretty far with the sterile tek, but from what I read, nothing bad. I personally don't go to such lengths. shit, I don't even tape off my gloves to long sleeves anymore.
also be careful not post back to back. you can edit your posts if you want to add more info. double posting is against the forum rules because it bumps your thread above all the other threads. Make sure you read the forum rules or risk the wrath of the mods.
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 1,138 topic views. 34 members, 200 guests and 43 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|