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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Gentics decay question
#22168735 - 08/31/15 11:39 AM (8 years, 4 months ago) |
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So i never made master slants or anything of my lc. All I have are Petri dishes. Some from witch are from bulk fruiting bags off Amazon.
I was planning on making prints of the following but from what I've been reading it's almost impossible to get good fruits mushrooms from spores.
I was planning on making spore prints from the following
Lion's mane Blue oysters Shiitake King oysters Miitake
From what I understand like us mycluim ages and is only good for so many transfers. That being the case how do you guys keep fresh genetics?
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mcchieftan
Part man, part mushroom



Registered: 09/08/05
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Re: Gentics decay question [Re: vatman]
#22168989 - 08/31/15 12:44 PM (8 years, 4 months ago) |
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We buy/trade slants/petris of young cell lines of proven fruiting cultures. We keep these on refrigerated slants and make new petris and slants from these when required.
So for example: for the moment we grow only King Oysters. I bought a culture from Mycelia.be and immediately prepared 10 slants. I used one of these slants to generate petri for making spawn until I'd used it all up and when I made the last batch of petri I made another bunch of slants from that petri. I put these in storage and then started using the second of my original slants... etc etc etc. Does that make sense?
Oh and as far as my research goes, you will be lucky to get a fruiting strain from an oyster spore print as the majority of viable osyter mycelium will not form fruitbodies... This may be the case for the rest but I am less certain..
Edited by mcchieftan (08/31/15 12:45 PM)
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Re: Gentics decay question [Re: mcchieftan]
#22169252 - 08/31/15 01:47 PM (8 years, 4 months ago) |
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><
Servers me right. I've done all my reading from the psychedelic sections of the forums as they have the most detailed guidance. Looks like ill head to the trading forums and try to get some masters
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drake89
Mushroom Magnate



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Re: Gentics decay question [Re: vatman]
#22169300 - 08/31/15 02:00 PM (8 years, 4 months ago) |
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Quote:
vatman said: ><
Servers me right. I've done all my reading from the psychedelic sections of the forums as they have the most detailed guidance. Looks like ill head to the trading forums and try to get some masters
just clone your fruits. that will give you fresh cultures.
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Re: Gentics decay question [Re: drake89]
#22169709 - 08/31/15 03:30 PM (8 years, 4 months ago) |
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Thought that was considered apart of the old genetics thus would be considered older tissue.
You are referring to stem tissue correct?
Also for something like lion's mane would you just take internal tissue?
Edit found some info on the forums that says to not do that. It is almost 5 years old though http://www.shroomery.org/forums/showflat.php/Number/14629222
Edited by vatman (08/31/15 03:50 PM)
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mikesethnobotany
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Re: Gentics decay question [Re: vatman] 1
#22170073 - 08/31/15 05:08 PM (8 years, 4 months ago) |
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Taking spore prints and generating good cultures would be a lot of work for edibles. You would be better off purchasing LC's from a place like SporeWorks or Ralphsters, plating them on petri dishes, then inoculating slants.
Anything grown out on grain has probably been expanded many times and would not be worth your money or time to make slants with.
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Gr0wer
always improving


Registered: 09/16/03
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Re: Gentics decay question [Re: mcchieftan]
#22170207 - 08/31/15 05:34 PM (8 years, 4 months ago) |
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Quote:
mcchieftan said:
Oh and as far as my research goes, you will be lucky to get a fruiting strain from an oyster spore print as the majority of viable osyter mycelium will not form fruitbodies... This may be the case for the rest but I am less certain..
Not completely true. I've fruited multispore culture of blues with good results. I think the main issue is the cultures are less predictable and calculated than isolates. With isolates you learn exactly what conditions and time the fruiting take place in.
The laundry basket in this pic is MS Blue oysters. Which later went into my buddies outdoor patch seen below. This was when i was still dialing in my chambers FAE so the stems are a bit long.

Edited by Gr0wer (08/31/15 05:39 PM)
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drake89
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Re: Gentics decay question [Re: vatman]
#22170414 - 08/31/15 06:13 PM (8 years, 4 months ago) |
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Quote:
vatman said: Thought that was considered apart of the old genetics thus would be considered older tissue.
You are referring to stem tissue correct?
Also for something like lion's mane would you just take internal tissue?
Edit found some info on the forums that says to not do that. It is almost 5 years old though http://www.shroomery.org/forums/showflat.php/Number/14629222
it may not be any better than the culture you started with. but it can't hurt to try.
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Re: Gentics decay question [Re: drake89]
#22170478 - 08/31/15 06:27 PM (8 years, 4 months ago) |
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@ Gr0wer Overall would you say ypu would try it again? General consensus seems to say stay away from that route.
@ drake89
From what I read the cells at the fruit would have devided up to that point. So cells at the fruit could be around after lets say 1 million devisions. When you stated that batch lets say you were at 250k devisions. From what I understand you would be cloning at the 1m mark thus genetics that are further down the road and are closer to failure.
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Gr0wer
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Re: Gentics decay question [Re: vatman]
#22171056 - 08/31/15 08:17 PM (8 years, 4 months ago) |
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I've considered it. I would probably do MS then clone a good fruit and make an isolate off that. Im sure the results will vary with different species but my blues did well with MS.
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Re: Gentics decay question [Re: Gr0wer]
#22171232 - 08/31/15 08:55 PM (8 years, 4 months ago) |
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That's what I was planning on doing but the nonfruiting / extremely low germ rate that people are claiming has me worried.
Also you don't know how many transfers your lc has gone through before you buy it.
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mcchieftan
Part man, part mushroom



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Re: Gentics decay question [Re: vatman]
#22172100 - 09/01/15 12:02 AM (8 years, 4 months ago) |
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@Grower: Do you have any yield info for "good results"? From my research I'd understood that a multistrain mycelium will yield less as some of the strains present may be non-fruiting...
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hamloaf
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Re: Gentics decay question [Re: vatman]
#22172465 - 09/01/15 03:54 AM (8 years, 4 months ago) |
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Quote:
vatman said: That's what I was planning on doing but the nonfruiting / extremely low germ rate that people are claiming has me worried.
Also you don't know how many transfers your lc has gone through before you buy it.
Pour 40 plates and inoculate all 40 plates with spores of a single specie if a low germination rate is your concern.
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Re: Gentics decay question [Re: hamloaf]
#22173325 - 09/01/15 10:03 AM (8 years, 4 months ago) |
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Quote:
hamloaf said:
Quote:
vatman said: That's what I was planning on doing but the nonfruiting / extremely low germ rate that people are claiming has me worried.
Also you don't know how many transfers your lc has gone through before you buy it.
Pour 40 plates and inoculate all 40 plates with spores of a single specie if a low germination rate is your concern.
From what I'm understanding is you have a low germination rate and also a low chance of that being able to fruit on top of that.
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Gr0wer
always improving


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Re: Gentics decay question [Re: vatman]
#22173772 - 09/01/15 12:11 PM (8 years, 4 months ago) |
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The basket I made was cardboard i would say the yield was average but I don't have any substrate and yeield weights. Also as I stated the grow chamber wasn't dialed in so yield wasn't optimal. If my buddy gets more fruits ill clone some flesh and see how it yields.
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CyclicAMP
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Re: Gentics decay question [Re: Gr0wer]
#22178804 - 09/02/15 03:59 PM (8 years, 4 months ago) |
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I make a master of anything new if i think i will want to keep it around. I use a bit different nutrient mix in the media for masters which includes nutritional yeast and some brown rice flour or rye flower. Make triplicates and fridge them. I have some that are still good after 5 years.
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