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OfflineJohnDoeKENTUCKY
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Best way for me to preserve my mushroom strains
    #22163646 - 08/30/15 07:40 AM (8 years, 4 months ago)

My first grow, about a month ago was a grea success. I spawned rye to coir, and did 5 trays getting pretty good yields. I inoculated the jars with a few spore syringes I bought. Now it is time for my second grow but I only have 3/4 of a syringe left.

What should I do to preserve my strain so I don't have to buy more syringes? I tried LC 5 days ago using Karo and water, injected 1cc in to 3 different pint jars with a polyfil lid. There is no growth in these jars still.  I have done G2G before in the past but I would rather not do it because I do not have a flow hood or SAB.    I was thinking about squirting water from a syringe (say 60ml) in to a colonized jar (I have a few colonized right now) then sucking the water back up threw a few more syringes, thus making an LC syringe ?

Also, I could not find this anywhere on the web, does horse manure make mushrooms more potent then say coir/verm?

Thanks,
JD


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OfflineMachiavelliavore
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Re: Best way for me to preserve my mushroom strains [Re: JohnDoeKENTUCKY]
    #22163682 - 08/30/15 07:58 AM (8 years, 4 months ago)

Quote:

JohnDoeKENTUCKY said:
My first grow, about a month ago was a grea success. I spawned rye to coir, and did 5 trays getting pretty good yields. I inoculated the jars with a few spore syringes I bought. Now it is time for my second grow but I only have 3/4 of a syringe left.

What should I do to preserve my strain so I don't have to buy more syringes? I tried LC 5 days ago using Karo and water, injected 1cc in to 3 different pint jars with a polyfil lid. There is no growth in these jars still.  I have done G2G before in the past but I would rather not do it because I do not have a flow hood or SAB.    I was thinking about squirting water from a syringe (say 60ml) in to a colonized jar (I have a few colonized right now) then sucking the water back up threw a few more syringes, thus making an LC syringe ?

Also, I could not find this anywhere on the web, does horse manure make mushrooms more potent then say coir/verm?

Thanks,
JD




General concensus is that poo does not make mushrooms more potent, but there are definitely some dedicated poo growers who will say otherwise.  I have no personal experience with poo.

I think your best option without an airbox would be to make a nice short container with a polyport (say out of a 1" pp5, make some BRF agar substitute or agar in there, put a drop from your syringe in, let it colonize, scrape mycellium with the needle (hence the short container,) then suck it out.

Otherwise I would say just sporeprint in a PC sterilized container, and try to work in still air conditions with it open as little as possible.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinetetherface
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Re: Best way for me to preserve my mushroom strains [Re: JohnDoeKENTUCKY]
    #22163685 - 08/30/15 08:00 AM (8 years, 4 months ago)

people claim it does but there is no "silver bullet" in regards to potency I've sampled fruits from several different subs and really haven't felt much difference I still feel that my CVG tubs are just as potent as my straw/poo/verm/g tubs:shrug: I really think it all depends on culture and in the case of cubes I eat a quail at least everytime I trip so I go deep either way:nyan:as far as saving your cultures without a SAB you cant do much of anything i'd build one if I were you it's an absolute necessity in this hobby and you can build a great one out of any5-10$ clear tote id recommend heating whatever you decide to use to make your holes though to avoid cracking your tub IME it makes life so much easier. other than that if you have clean spawn  colonized already you could refrigerate them and use them as masters and G2G them out as needed(requires SAB) but i'd recommend taking a clone from your current grow and making masters outta that otherwise youd just be going thru a hassle to preserve a MS culture when you could just re inoculate to get the same results:shrug:you cant do LC without a SAB anyways but what did you use for inoculate?if it was the MS syringe save yourself the hassel and toss em MS to LC is a waste of spores and rarely works out I love 9er tek cloning for inoculate then I put that to pf cakes to refrigerate and slurry out as needed pf cakes hold up well in the fridge and take up little space in the cooler in the fridge so I can have 4 PF 1/2 pints and slurry them out as needed I usually slurry 1 pf cake to 20 3/4 full qt jars for a 4-5 tub run its possible but you really need a SAB bro you cant do anything reliably without it


--------------------


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OfflinePussyFart
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Re: Best way for me to preserve my mushroom strains [Re: JohnDoeKENTUCKY]
    #22164598 - 08/30/15 12:24 PM (8 years, 4 months ago)

Quote:

JohnDoeKENTUCKY said:
What should I do to preserve my strain so I don't have to buy more syringes?



AGAR

CLONING

ISOLATION

SLANTS


Edited by PussyFart (08/30/15 12:25 PM)


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OfflineDeTwizzle
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Re: Best way for me to preserve my mushroom strains [Re: PussyFart]
    #22164639 - 08/30/15 12:35 PM (8 years, 4 months ago)

:whathesaid:

:adventuretime:


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OfflineJohnDoeKENTUCKY
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Re: Best way for me to preserve my mushroom strains [Re: tetherface]
    #22165404 - 08/30/15 04:22 PM (8 years, 4 months ago)

I am using the MS syringe to inoculate. Btw, do you think that polyfil lids are the way to go or should I use micropore?


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OfflinecronicrFacebook
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Re: Best way for me to preserve my mushroom strains [Re: JohnDoeKENTUCKY]
    #22165409 - 08/30/15 04:23 PM (8 years, 4 months ago)

poly works great for grain spawn


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineJohnDoeKENTUCKY
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Re: Best way for me to preserve my mushroom strains [Re: Machiavelliavore]
    #22165591 - 08/30/15 05:21 PM (8 years, 4 months ago)

What all do I need to do this? I am making an SAB right now and I have 3 empty syringes, can I use honey or Karo as the LC? Should I use Petri dishes?


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OfflineJohnDoeKENTUCKY
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Re: Best way for me to preserve my mushroom strains [Re: cronicr]
    #22165600 - 08/30/15 05:23 PM (8 years, 4 months ago)

Is poly a good thing to use for LC master jars as well? I bought some SFD's and cut them up into smaller circles then used hi temp caulk to hold them down. What's best for LC? What's best way to innoc LC besides using piece of fruit?


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OfflinecronicrFacebook
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Re: Best way for me to preserve my mushroom strains [Re: JohnDoeKENTUCKY]
    #22165618 - 08/30/15 05:30 PM (8 years, 4 months ago)

whatmans or syringe filters are best suited for lc's, when typical grain style filters like tyvek/sfds/poly get wet they can let things like bacteria in your lc, lc is always best inoculated with agar but some get by with spores or clones, not something i'm willing to do though


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinePussyFart
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Re: Best way for me to preserve my mushroom strains [Re: JohnDoeKENTUCKY]
    #22165699 - 08/30/15 05:47 PM (8 years, 4 months ago)

Quote:

JohnDoeKENTUCKY said:
What's best for LC? What's best way to innoc LC besides using piece of fruit?



That isn't even a good way to make an LC.....using a clean, colonized agar wedge is the best way....


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