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Invisiblemicro
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Sterile room with antichamber simpler than a glovebox
    #22163365 - 08/30/15 03:10 AM (8 years, 4 months ago)

When I was growing in an apartment, we had a room dedicated to shrooms that were colonizing. Of course we cleaned it, but this was around 9/11 when the crackpot Congressman told people to cover their houses with plastic sheeting and duct tape. This gave me an idea.

We bought plastic sheeting (cheap, so we got heavy-duty stuff) and duct taped and staple-gunned it floor to ceiling and walls. We had an "anti-chamber" with sterile gowns and it was sealed with Velcro so when you in the room  there's minimal contmination... or none.

There has to be a heater in there around 85F which creates a positive pressure environment anyway. Nothing is getting in. I think we had a HEPA filter in there too (cheap floor model but new, so clean). The inner room was our work area.

Hardly any problems; I think I was doing plates in the air (mind you I was suited up -- 2 pairs of gloves, sleeves, mask, hair net, pant legs... I didn't go with the BL4 stuff though -- that's way overboard (and no air supply).

I also cheated -- we sprayed it with 2% paracetic acid an hour before work, You can't get the stuff... You can make it but don't try. It will kill you very easily, esp. in high concentrations which is the only way it stores in amber bottles (I think 33% IRC).

Just an idea


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Re: Sterile room with antichamber simpler than a glovebox [Re: micro]
    #22170864 - 08/31/15 07:40 PM (8 years, 4 months ago)

You can get peroxyacetic acid. It's sanitizer for the brewing and wine making. It comes 26% you dilute it 1oz 5 gal. At full strength it turns your skin white in seconds and then burns until you run to a sink. The fumes are so strong at full strength you would never harm yourself unless you spill it on yourself.

A heater is not positive pressure unless it's got an external intake  otherwise its just a fan in a room.

Even still call up all the labs cus you just found a way to save them 1000s of dollars doing the same thing..


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Invisiblemicro
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Re: Sterile room with antichamber simpler than a glovebox [Re: bodhisatta]
    #22171193 - 08/31/15 08:47 PM (8 years, 4 months ago)

Uh. Actuallly, when I was working at Tufts in molecular biology that is how we did it. I'll admit a small HEPA filter is cheesy but better than nothing and if its new, it should filter pretty well. The hot air from the heater creates a positive pressure environment -- this is obvious since the plastic bulges out.

Or am I missing some point here? Seems like Devil's Advocate, but it works :p

And yeah, surgery rooms have tons of expensive shit built in. I was going for simplicity.

A glovebox isn't sterile anyway, even if you add UV light,it's  just a very clean space.

And you can BUY paracetic (peroxyacetic) acid OTC? Source? This would surprise me.

Relatively easy to make though. EDIT: assuming you can get GAA and strong H2O2, or know how to make the letter.

2% solution (based on the concentrated stuff) will kill EVERYTHING nd break down into vinegar and water in an hour. Another useful win for clandestine mycology (if someone can make the stuff).

And I was told it' deadly but OSHA also calls a hazmat team if you break a merury thermometer :rolleyes:


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Edited by micro (08/31/15 08:53 PM)


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Offlinedrake89
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Re: Sterile room with antichamber simpler than a glovebox [Re: micro]
    #22175173 - 09/01/15 06:44 PM (8 years, 4 months ago)

i saw doc holliday loaned a farmer friend a unit that fogged with that stuff for full grow room decontamination.  i don't think it's impossible to come by.  I see where you're coming from.  As my lab that I inoculate 100 bags in everyday rarely gets thoroughly cleaned.  I just keep moldy shit out.  And keep my work table sanitized.  There's spores floating everywhere until I turn on my flowhood.  I don't shower or change clothes before inoculating.  I do make sure to be clean when I unload the sterilizers in the morning, though.


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Re: Sterile room with antichamber simpler than a glovebox [Re: drake89]
    #22175882 - 09/01/15 09:17 PM (8 years, 4 months ago)

We get 55g drums from a place down the road literally. They would sell you less no problem. Maybe not some kid but no problem if you said you made wine etc...


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Invisiblemicro
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Re: Sterile room with antichamber simpler than a glovebox [Re: bodhisatta]
    #22176725 - 09/02/15 01:09 AM (8 years, 4 months ago)

Oh, wow. I always thought you needed a license for that.

Though I think strong peroxide and acetic acid might do the trick.

It really *is* useful stuff, but then the room smells like vinegar.


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Invisiblemicro
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Re: Sterile room with antichamber simpler than a glovebox [Re: bodhisatta]
    #22176727 - 09/02/15 01:10 AM (8 years, 4 months ago)

Oh, wow. I always thought you needed a license for that.

Though I think strong peroxide and acetic acid might do the trick.

It really *is* useful stuff, but then the room smells like vinegar.

Quote:

bodhisatta said:A heater is not positive pressure unless it's got an external intake  otherwise its just a fan in a room.




Did I forget to address this one?

Hot air expands.


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Re: Sterile room with antichamber simpler than a glovebox [Re: micro] * 1
    #22182615 - 09/03/15 11:30 AM (8 years, 4 months ago)

Quote:

micro said:
When I was growing in an apartment, we had a room dedicated to shrooms that were colonizing. Of course we cleaned it, but this was around 9/11 when the crackpot Congressman told people to cover their houses with plastic sheeting and duct tape. This gave me an idea.

We bought plastic sheeting (cheap, so we got heavy-duty stuff) and duct taped and staple-gunned it floor to ceiling and walls. We had an "anti-chamber" with sterile gowns and it was sealed with Velcro so when you in the room  there's minimal contmination... or none.

There has to be a heater in there around 85F which creates a positive pressure environment anyway. Nothing is getting in. I think we had a HEPA filter in there too (cheap floor model but new, so clean). The inner room was our work area.

Hardly any problems; I think I was doing plates in the air (mind you I was suited up -- 2 pairs of gloves, sleeves, mask, hair net, pant legs... I didn't go with the BL4 stuff though -- that's way overboard (and no air supply).

I also cheated -- we sprayed it with 2% paracetic acid an hour before work, You can't get the stuff... You can make it but don't try. It will kill you very easily, esp. in high concentrations which is the only way it stores in amber bottles (I think 33% IRC).

Just an idea




I completely fail to see how this is simpler than a glovebox or, in my case, a SAB. On second thought...I still fail to see it.


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Edited by champinhom (09/03/15 11:30 AM)


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Re: Sterile room with antichamber simpler than a glovebox [Re: champinhom] * 2
    #22183208 - 09/03/15 01:51 PM (8 years, 4 months ago)

Quote:

champinhom said:
I completely fail to see how this is simpler than a glovebox or, in my case, a SAB.



That's because it's not....


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OfflineChk
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Re: Sterile room with antichamber simpler than a glovebox [Re: micro]
    #22186520 - 09/04/15 06:10 AM (8 years, 4 months ago)

Quote:

Did I forget to address this one?

Hot air expands.




Yes, and once it's all expanded, it's not positive pressure anymore, unless you got an external intake


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InvisibleToadstool5
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Re: Sterile room with antichamber simpler than a glovebox [Re: Chk] * 1
    #22189170 - 09/04/15 05:40 PM (8 years, 4 months ago)

Quote:

Yes, and once it's all expanded, it's not positive pressure anymore, unless you got an external intake.




I also dont think simple convection would work to keep out MCU's like flies and gnats. The air currents from a fan alone could cause back-draft into the room.

With a SAB i just wipe my counter with 3% bleach, run a small HEPA 24/7, turn off the HEPA, allow the air to settle for an hour, and work. It's so easy i feel guilty sometimes.

A SAB is also portable and is easily stored away when not being used. :shrug:


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Re: Sterile room with antichamber simpler than a glovebox [Re: Toadstool5]
    #22211923 - 09/09/15 11:58 AM (8 years, 4 months ago)

What happens when the air in the room has expanded as much as its going to? It's no longer positive pressure and you better hope you never drop a degree or two in there and the air contracts sucking everything from everywhere it can.

This sounds like the oven tek for a whole room honestly.
For around the same price go buy a small inline fan, intake from another room or window and output through a hepa and you will have pos pressure if you indeed have a sealed room


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Re: Sterile room with antichamber simpler than a glovebox [Re: maddchef]
    #22212026 - 09/09/15 12:21 PM (8 years, 4 months ago)

OP trolling us all real hard.  Just like the whole "you don't need a lab" thread.  Why is everyone so fucking stupid?


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Re: Sterile room with antichamber simpler than a glovebox [Re: drake89]
    #22212030 - 09/09/15 12:22 PM (8 years, 4 months ago)

We are all shouting into a vacuum in here.  You can't tell a mushroom head shit, so I think that should be the title of this thread.


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Re: Sterile room with antichamber simpler than a glovebox [Re: drake89]
    #22212058 - 09/09/15 12:30 PM (8 years, 4 months ago)

Also just struck me his shroom room is 85°....


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Re: Sterile room with antichamber simpler than a glovebox [Re: maddchef]
    #22244803 - 09/16/15 08:27 AM (8 years, 4 months ago)

Quote:

maddchef said:
What happens when the air in the room has expanded as much as its going to? It's no longer positive pressure and you better hope you never drop a degree or two in there and the air contracts sucking everything from everywhere it can.




As long as there is airflow into another cooler room (e.g. through windows or doors), the heater will maintain positive pressure so long as the temperature doesn't fluctuate readily.

I also think the OP probably calls it simpler than a glove box because working in a confined glovebox can get really frigging annoying especially with grain jars larger than quart size (I have yet to find a clear storage bin deep enough in my area to work with half gallon to gallon size jars).


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Invisiblemicro
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Re: Sterile room with antichamber simpler than a glovebox [Re: mackrel]
    #22247576 - 09/16/15 07:29 PM (8 years, 4 months ago)

p much

it wasn't very hard to duct tape plastic sheeting to the ceiling :V

(or we may have used a staple gun, I forget)

but yeah, just wanted positive pressure and a room to work in

"antichamber" door was another sheet with Velcro

we also had sterile gowns I stole from the lab I worked in, these could obviously be substituted

also, re: convections? where, under the plastic sheet and back in? :V

use your imagination; it's circling around inside the room

make sure the heater is new/clean


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Edited by micro (09/16/15 07:35 PM)


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