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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Quote:
tetherface said: would it be acceptable to clone a 1st flush fruit from this clone? I would imagine some degradation of a culture from repeated cloning, not in this situation as im only 2 generations from spore right?
why not just go back to the plate?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Re: 100% BE????? [Re: cronicr]
#22164984 - 08/30/15 02:15 PM (8 years, 4 months ago) |
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I could just wasn't sure which is better 1. to clone another first flush fruit or 2. go back to the original plate which is horribly overgrown, I also have a plate that I've been trying to isolate as a mono culture so I could use it to inoculate some grain jars at my next transfer but its from a different clone. I just wasn't sure what the community considers a "good" culture and was just wondering how BE weighs in on your guys decision making if it does at all. thanks for the responses guys
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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I wouldnt clone a clone, take care of your plates because if it is a winner you want it in good shape
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Quote:
tetherface said: I always wondered why we didn't factor in grain to BE
what? of course we do! if not then every tub with 1 brick coir 2qt verm would only have to give 3-4oz for 100% BE. (650g coir + a cpl 100 grams for the verm.
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Quote:
spacechildo said:
Quote:
tetherface said: I always wondered why we didn't factor in grain to BE
what? of course we do! if not then every tub with 1 brick coir 2qt verm would only have to give 3-4oz for 100% BE. (650g coir + a cpl 100 grams for the verm.
really? see that makes sense to me but its always been explained as dry weight of sub compared to wet weight of first flush so am I wrong cause I think that's what I read in TMC??/?
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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the substrate means the block you're fruiting from. you add the weight of the brf and verm in pf-cakes BE calculations. more spawn gives you more mushrooms but spawn also weighs quite a bit even dry, 150-200g pr cup which = ~1 qt jar prepped spawn.
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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that makes perfect sense to me but like I said I've never heard anyone else explain it like that but anyways in response to CRON I just pulled that overgrown plate out of the tote I keep my spawn in and its pinning on agar well actually off the glass but does that indicate good or bad? the plate was clean (I think) before it grew out should I clone 1 of the pins? I just made up a bunch of pasty plates for the "communal grow" tomorrow so I have plates on hand or should I just start transferring myc and isolating?  what do you guys think? I've never let a plate do this before so i'm not sure what my best options are?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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It's not wet weight of first flush that calculates BE 
See now mach just got you confused.
DRY SUB -> WET HARVEST
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Re: 100% BE????? [Re: Inocuole]
#22165207 - 08/30/15 03:27 PM (8 years, 4 months ago) |
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oh man i'm getting facepalmed...lol I thought it was wet weight first flush in relation to dry substrate weight (not including spawn) but space said it does include spawn and he knows his shit so who is right do you include dry weight of grain spawn? is this culture a super fail because I didn't include spawn in my original BE figures? and whats your guys take on where to go from here should I clone a pin off the original overgrown plate pictured above?thanks guys I appreciate it
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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it's your total harvest not just your first flush and yes grain spawn, casing payers...everything should be added that's a part of the substrate
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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tether, consider this.
if I have a qt jar with colonized grains, and it has a bit of bacteria in it so I dont wanna spawn it. I put ~5-10gram verm on top and fruit it like a mudabottle.
I get 100grams fresh mushrooms. what's my BE? 100g mush/10g verm x 100% = 1000%Did I just knock it out of the ball park?
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Quote:
tetherface said: and whats your guys take on where to go from here should I clone a pin off the original overgrown plate pictured above?thanks guys I appreciate it
the myc on the plate has spent far less energy than the finished mushroom has. I'd still use the plate. remember to put it in the fridge next time!
oh and cron just nailed it!
Edited by spacechildo (08/30/15 03:31 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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as for your plate you could chuck one of those pins on a new plate, next time don't let it grow out like that
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Re: 100% BE????? [Re: cronicr]
#22165271 - 08/30/15 03:46 PM (8 years, 4 months ago) |
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thanks guys for taking the time to explain it thouroughly so ya im a failure lol ill keep on searchin for a good culture and im gonna take the pin to a plate and maybe do a few transfers from the overgrown plate (I don't have a clue on where to take myc from since its so overgrown)and ya fridge FTW from now on that plate kinda just got left behind in my box when I made a couple LC's with another one that ive been using as inoculate all month so I kinda just forgot about it on another note i'm working on a monoculture that produced that fatty in my sig so at least I have options but the way cron implied its not good practice to clone a clone on the 1st flush?
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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you want to store as young a culture as possible, thats why you put the plate in the fridge once its clean and then just have it sit cold while the grains are colonizing etc. after first harvest you decide if its a keeper (make a slant of it) or not.
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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right on bro you always give me good info my mutha fuckin man!!!!!
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: 100% BE????? [Re: cronicr]
#22217386 - 09/10/15 03:33 PM (8 years, 4 months ago) |
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When weighing your spawn, do you weigh it unprepped or prepped (soaked or simmered)? colonized or uncolonized?
Oh and I assume you don't weigh the casing material?
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ShroominMe
Stranger

Registered: 05/03/15
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Re: 100% BE????? [Re: FriedEgg]
#22219292 - 09/10/15 10:59 PM (8 years, 4 months ago) |
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Sorry, worst post ever
Edited by ShroominMe (09/11/15 03:48 PM)
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